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Contribution of decreased expression of Ku70 to enhanced radiosensitivity by sodium butyrate in glioblastoma cell line (U251)

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Summary

The present study investigated the enhanced radiosensitivity of U-251 cells induced by sodium butyrate (NaB) and its possible mechanisms. Increased radiosensitivity of U251 cells was examined by clonogenic cell survival assays. The expression of Ku70 mRNA and protein was detected by using RT-PCR and Western blotting respectively. γ-H2AX foci were measured at different time points after ionizing irradiation alone or combined with NaB treatment. The results showed that cell survival rate was significantly reduced, both D0 and Dq values were decreased (D0: 1.43 Gy vs. 1.76 Gy; Dq: 1.22 Gy vs. 2.05 Gy) after the combined treatment as compared with irradiation alone, and sensitivity enhancing ratio (SER) reached 1.23. The average number of γ-H2AX foci per cell receiving the combined treatment was significantly increased at different time points, and the expression levels of Ku70 mRNA and protein were suppressed by NaB in a dose-dependent manner. It was concluded that enhanced radiosensitivity induced by NaB involves an inhibited expression of Ku70 and an increase in γ-H2AX foci, which suggests decreased ability in DSB repair.

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Authors and Affiliations

  1. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430023, China

    Yuhui Li  (李宇辉), Hongxia Zhou  (周红霞), Enming Xing  (邢恩明), Meera Dassarath, Jinghua Ren  (任精华), Xiaorong Dong  (董晓荣), Hongli Liu  (刘红利), Kunyu Yang  (杨坤禹) & Gang Wu  (伍 钢)

  2. Department of Oncology, Wuhan Central Hospital, Wuhan, 430014, China

    Yuhui Li  (李宇辉)

  3. Department of Oncology, Queen Victoria Hospital, Candos, Quatre-Bornes, Mauritius

    Meera Dassarath

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  1. Yuhui Li  (李宇辉)
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  2. Hongxia Zhou  (周红霞)
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  3. Enming Xing  (邢恩明)
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  5. Jinghua Ren  (任精华)
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  6. Xiaorong Dong  (董晓荣)
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  7. Hongli Liu  (刘红利)
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  8. Kunyu Yang  (杨坤禹)
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  9. Gang Wu  (伍 钢)
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Correspondence to Kunyu Yang  (杨坤禹).

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Both authors contributed equally to this work.

This project was supported by a grant from National Natural Sciences Foundation of China (No. 30870739).

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Li, Y., Zhou, H., Xing, E. et al. Contribution of decreased expression of Ku70 to enhanced radiosensitivity by sodium butyrate in glioblastoma cell line (U251). J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 359–364 (2011). https://doi.org/10.1007/s11596-011-0381-8

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  • DOI: https://doi.org/10.1007/s11596-011-0381-8

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