Skip to main content
SpringerLink
Log in

Murine cytomegalovirus IE3 protein interacts with Ankrd17

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

Murine cytomegalovirus (MCMV) IE3 protein is a multifunctional viral protein that interacts with several target proteins of both viral and host cellular origin. To investigate the biological function of IE3 in the pathogenesis of the brain disorders caused by CMV, a screening for host cellular proteins that could interact with IE3 was performed. By yeast two-hybrid screening, ankyrin repeats domain 17 (Ankrd17, also known as Gtar) was identified as a host factor that could interact with IE3. This interaction was verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion. Mapping analysis suggested that the 1–148 residues of IE3 were responsible for the interaction. These results suggested that the interaction between Ankrd17 and IE3 may play a key role in the pathogenesis of MCMV-associated disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Griffiths P. Cytomegalovirus infection of the central nervous system. Herpes, 2004,11(Suppl 2):95A–104A

    PubMed  Google Scholar 

  2. Hollier L M, Grissom H. Human herpes viruses in pregnancy: cytomegalovirus, Epstein-Barr virus, and varicella zoster virus. Clin Perinatol, 2005,32(3):671–696

    Article  PubMed  Google Scholar 

  3. Sinzer C, Jahn G. Human cytomegalovirus cell tropism and pathogenesis. Intervirology, 1996,39(5–6):302–319

    Google Scholar 

  4. Reddehase MJ, Simon CO, Seckert CK, et al. Murine model of cytomegalovirus latency and reactivation. Curr Top Microbiol Immunol, 2008,325:315–331

    Article  PubMed  CAS  Google Scholar 

  5. Tsutsui Y, Kosugi I, Kawasaki H. Neuropathogensis in cytomegalovirus infection: indication of the mechanisms using mouse models. Rev Med Virol, 2005,15(5):327–345

    Article  PubMed  CAS  Google Scholar 

  6. Reddehase MJ, Podlech J, Grzimek NK. Mouse models of cytomegalovirus latency: overview. J Clin Virol, 2002,25(Suppl 2):S23–36

    Article  PubMed  CAS  Google Scholar 

  7. Li RY, Baba S, Kosugi I, et al. Activation of murine cytomegalovirus immediate-early promoter in cerebral ventricular zone and glial progenitor cells in transgenic mice. Glia, 2001,35(1):41–52

    Article  PubMed  CAS  Google Scholar 

  8. Ghazal P, Messerle M, Osborn K, et al. An essential role of the enhancer for murine cytomegalovirus in vivo growth and pathogenesis. J Virol, 2003,77(5):3217–3228

    Article  PubMed  CAS  Google Scholar 

  9. Angulo A, Ghazal P, Messerle M. The major immediate-early gene ie3 of mouse cytomegalovirus is essential for viral growth. J Virol, 2000,74(23):11129–11136

    Article  PubMed  CAS  Google Scholar 

  10. Tang Q, Li L, Maul GG. Mouse cytomegalovirus early M112/113 proteins control the repressive effect of IE3 on the major immediate-early promoter. J Virol, 2005,79(1): 257–263

    Article  PubMed  CAS  Google Scholar 

  11. Benoit RM, Wilhelm RN, Scherer-Becker D, et al. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids. Protein Expr Purif, 2006,45(1):66–71

    Article  PubMed  CAS  Google Scholar 

  12. Messerle M, Buhler B, Keil GM. et al. Structural organization, expression, and functional characterization of the murine cytomegalovirus immediate-early gene 3. J Virol, 1992,66(1):27–36

    PubMed  CAS  Google Scholar 

  13. Maul GG, Negorev D. Differences between mouse and human cytomegalovirus interactions with their respective hosts at immediate early times of the replication cycle. Med Microbiol Immunol, 2008,197(2):241–249

    Article  PubMed  Google Scholar 

  14. Martínez FP, Cosme RS, Tang Q. Murine cytomegalovirus major immediate-early protein 3 interacts with cellular and viral proteins in viral DNA replication compartments and is important for early gene activation. J Gen Virol, 2010,91(Pt 11):2664–2676

    Article  PubMed  Google Scholar 

  15. Hou SC, Chan LW, Chou YC, et al. Ankrd17, an ubiquitously expressed ankyrin factor, is essential for the vascular integrity during embryogenesis. FEBS Lett, 2009, 583(17):2765–2771

    Article  PubMed  CAS  Google Scholar 

  16. Deng M, Li F, Ballif BA, Li S, et al. Identification and functional analysis of a novel cyclin e/cdk2 substrate ankrd17. J Biol Chem, 2009,284(12):7875–7888

    Article  PubMed  CAS  Google Scholar 

  17. Guan H, Kiss-Toth E. Advanced technologies for studies on protein interactomes. Adv Biochem Engin/Biotechnol, 2008,110:1–24

    Article  CAS  Google Scholar 

  18. Ratushny V, Golemis E. Resolving the network of cell signaling pathways using the evolving yeast two-hybrid system. Biotechniques, 2008,44(5):655–662

    Article  PubMed  CAS  Google Scholar 

  19. Luo D, Shu SN, Tian J, et al. Experimental study of the influence of murine cytomegalovirus infection on cell cycle progression and cyclins expression of neural stem cells in vitro. Chin J Neuroanatomy, 2010,26(1):1–8

    Google Scholar 

  20. Wiebusch L, Neuwirth A, Grabenhenrich L, et al. Cell cycle-independent expression of immediate-early gene 3 results in G1 and G2 arrest in murine cytomegalovirus-infected cells. J Virol, 2008,82(20):10188–10198

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Hui Wang  (王 慧), Xinglou Liu  (刘兴楼), Sainan Shu  (舒赛男), Ju Zhang  (张 菊), Yongjian Huang  (黄永建) & Feng Fang  (方 峰)

Authors
  1. Hui Wang  (王 慧)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xinglou Liu  (刘兴楼)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sainan Shu  (舒赛男)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ju Zhang  (张 菊)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yongjian Huang  (黄永建)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Feng Fang  (方 峰)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Feng Fang  (方 峰).

Additional information

This project was supported by a grant from National Natural Sciences Foundation of China (No. 30671859).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, H., Liu, X., Shu, S. et al. Murine cytomegalovirus IE3 protein interacts with Ankrd17. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 285–289 (2011). https://doi.org/10.1007/s11596-011-0368-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-011-0368-5

Key words

Search

Navigation