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Von Willebrand factor antigen and ADAMTS13 activity assay in pregnant women and severe preeclamptic patients

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Summary

The present study examined von Willebrand factor (vWF) levels and ADAMTS13 activity in pregnant and severe preeclamptic women in order to shed light on the prothrombotic state in severe preeclampsia. Thirty healthy women of childbearing age, 22 second trimester pregnant women, 30 third trimester pregnant women and 10 severe preeclamptic patients were recruited in this study. ADAMTS13 activity was determined by the FRETS-vWF73 assay and vWF antigen (vWF:Ag) levels by an enzyme-linked immunosorbent assay. The results showed that there were statistically significant differences in plasma vWF antigen levels between the severe preeclamptic and third trimester pregnant women, between third and second trimester pregnant women (P<0.05). The third trimester pregnant women had significantly lower plasma ADAMTS13 activity than second trimester pregnant women (P<0.05). Nevertheless, no significant differences in plasma ADAMTS13 activity were found between severe preeclamptic patients and the third trimester pregnant women (P>0.05). In conclusion, plasma ADAMTS13 activity is normal in severe preeclampsia despite the increased vWF:Ag levels. Prothrombotic state is involved in the pathogenesis of severe preeclampsia, as a result of endothelial injury.

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References

  1. Sadler JE, Moake JL, Miyata T, et al. Recent advances in thrombotic thrombocytopenic purpura. Hematologyo (Am Soc Hematol Educ Program), 2004, 2004(1):407–423

    Google Scholar 

  2. Fujikawa K, Suzuki H, McMullen B, et al. Purification of human von willebrand factor-cleaving protease and its identification as a new member of the metalloproteinase family. Blood, 2001,98(6):1662–1666

    Article  CAS  PubMed  Google Scholar 

  3. Molvarec A, Rigo J, Boze T, et al. Increased plasma von Willebrand factor antigen levels but normal von Wille brand factor cleaving protease (ADAMTS13) activity in preeclampsia. Thromb Haemost, 2009,101(2): 305–311

    CAS  PubMed  Google Scholar 

  4. Mannucci PM, Canciani MT, Forza I, et al. Changes in health and disease of the metalloprotease that cleaves von Willebrand factor. Blood, 2001,98(9):2730–2735

    Article  CAS  PubMed  Google Scholar 

  5. Furlan M, Lammle B. Assays of von Willebrand factor-cleaving protease: a test for diagnosis of familial and acquired thrombotic thrombocytopenic purpura. Semin Thromb Haemost, 2002,28(2):167–172

    Article  CAS  Google Scholar 

  6. Rick ME, Moll S, Taylor MA, et al. Clinical use of a rapid collagen binding assay for von Willebrand factor cleaving protease in patients with thrombotic thrombocytopenic purpura. Thromb Haemost, 2002,88(4): 598–604

    CAS  PubMed  Google Scholar 

  7. Kokame K, Matsumoto M, Fujimura Y, et al. vWF73, a region from D1596 to R1668 of von Willebrand factor, provides a minimal substrate for ADAMTS-13. Blood, 2004,103(2):607–612

    Article  CAS  PubMed  Google Scholar 

  8. Kokame K, Nobe Y, Kokubo Y, et al. FRETS-vWF73, a first fluorogenic substrate for ADAMTS13 assay. Br J Haematol, 2005,129(1):93–100

    Article  CAS  PubMed  Google Scholar 

  9. Raife TJ, Cao W, Atkinson BS, et al. Leukocyte proteases cleave von Willebrand factor at or near the ADAMTS13 cleavage site. Blood, 2009,114(8):1666–1674

    Article  CAS  PubMed  Google Scholar 

  10. Shankaran H, Alexandridis P, Neelamegham S. Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-assocaition of von Willebrand factor in suspension. Blood, 2003,101(7):2637–2645

    Article  CAS  PubMed  Google Scholar 

  11. Andrews RK, Berndt MC. Platelet physiology and thrombosis. Thromb Res, 2004,114(5–6):447–453

    Article  CAS  PubMed  Google Scholar 

  12. Donadelli R, Orje JN, Capoferri C, et al. Size regulation of von Willebrand factor-mediated platelet thrombi by ADAMTS13 in flowing blood. Blood, 2006,107(5):1943–1950

    Article  CAS  PubMed  Google Scholar 

  13. Oleksowicz L, Bhagwati N, DeLeon-Fernandez M. Deficient activity of von Willebrand’s factor-cleaving protease in patients with disseminated malignancies. Cancer Res, 1999, 59(9):2244–2250

    CAS  PubMed  Google Scholar 

  14. George JN. The association of pregnancy with thrombotic thrombocytopenic purpura—hemolytic uremic syndrome. Curr Opin Hematol, 2003,10(5):339–344

    Article  PubMed  Google Scholar 

  15. Mariarosaria I, Patrizia S, Antonio DA, et al. A post-partum hemolytic-uremic-like-syndrome in a patient with pre-eclampsia: Description of a clinical case. Transfus Apher Sci, 2006,34(1):11–14

    Article  Google Scholar 

  16. McMinn JR, George JN. Evaluation of women with clinically suspected thrombotic thrombocytopenic purpura—hemolytic uremic syndrome during pregnancy. J Clin Apheresis, 2001,16(4):202–209

    Article  CAS  PubMed  Google Scholar 

  17. Hulstein JJ, Van Runnard Heimei PJ, Franx A, et al. Acute activation of the endothelium results in increased levels of active von Willebrand factor in hemolysis, elevated liver enzymes and low platelets (HELLP) syndrome. Thromb Haemost, 2006,4(12):2569–2575

    Article  CAS  Google Scholar 

  18. Furlan M, Robles R, Galbusera M, et al. von Willebrand factor-cleaving protease in thrombotic thrombocytopenic purpura and the hemolytic-uremic syndrome. N Engl J Med, 1998,339(22):1578–l584

    Article  CAS  PubMed  Google Scholar 

  19. Tsai HM, Lian EC. Antibodies to von Willebrand factor- cleaving protease in acute thrombotic thrombocytopcnic purpura. N Engl J Med, 1998,339(22):1585–1594

    Article  CAS  PubMed  Google Scholar 

  20. Mahdian R, Rayes J, Girma JP, et al. Comparison of FRETS-vWF73 to full-length vWF as a substrate for ADAMTS13 activity measurement in human plasma samples. Thromb Haemost,2006,95(6):1049–1051

    CAS  Google Scholar 

  21. Nishio K, Anderson PJ, Zheng XL, et al. Binding of platelet glycoprotein Iba to von Willebrand factor domain A1 stimulates the cleavage of the adjacent domain A2 by ADAMTS13. Proc Natl Acad Sci USA, 2004,101(29), 10578-10583

    Google Scholar 

  22. Zhuang X, Ha T, Kim HD, et al. Fluorescence quenching: A tool for single—molecule protein-folding study. Proc Natl Acad Sci USA, 2000,97(26):14241–14244

    Article  CAS  PubMed  Google Scholar 

  23. Zhang L, Lawson H, Harish V, et al. Creation of a recombinant peptide substrate for fluorescence resonance energy transfer-based protease assays. Anal Biochem, 2006,358(2): 298–300

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Dandan Zhang  (张丹丹), Juan Xiao  (肖 娟), Juanjuan Chen  (陈娟娟), Tao Liu  (刘 涛), Zongzhi Yin  (尹宗智), Danping Gao  (高单萍), Qiong Liu  (刘 琼), Jihui Ai  (艾继辉) & Suhua Chen  (陈素华)

  2. Department of Obstetrics and Gynecology, the First Hospital of Wuhan, Wuhan, 430022, China

    Haoliang Huang  (黄浩梁)

Authors
  1. Dandan Zhang  (张丹丹)
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  2. Juan Xiao  (肖 娟)
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  3. Haoliang Huang  (黄浩梁)
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  4. Juanjuan Chen  (陈娟娟)
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  5. Tao Liu  (刘 涛)
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  6. Zongzhi Yin  (尹宗智)
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  7. Danping Gao  (高单萍)
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  8. Qiong Liu  (刘 琼)
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  9. Jihui Ai  (艾继辉)
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  10. Suhua Chen  (陈素华)
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Corresponding author

Correspondence to Suhua Chen  (陈素华).

Additional information

This project was supported by grants from National Natural Sciences Foundation of China (No.30672243, No.30671093) and Natural Sciences Foundation of Hubei Province (No. ZR-Y1271).

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Zhang, D., Xiao, J., Huang, H. et al. Von Willebrand factor antigen and ADAMTS13 activity assay in pregnant women and severe preeclamptic patients. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 777–780 (2010). https://doi.org/10.1007/s11596-010-0657-4

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  • DOI: https://doi.org/10.1007/s11596-010-0657-4

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