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Effects of Ad-p27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice

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Summary

In this study, the mechanism by which Ad-p27mt inhibits the growth, invasion and metastasis of transplanted liver tumor was studied by examining the effects of Ad-27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice. The model of transplanted hepatic tumor was established in nude mice. The mice were then divided into three groups, which were injected with PBS, Ad-LacZ and Ad-p27mt and the growth of the transplanted liver tumor was observed. The expressions of P27, Bax and Bcl-2 proteins were detected by Western blotting and the expressions of VEGF and MMP-9 were immunohistochemically determined. Our result showed that the tumor size, expressions of Bax, Bcl-2 proteins, VEGF and MMP-9 were all lower than those in PBS and Ad-LacZ groups and the differences were statistically significant (P<0.05). Our study suggested that Ad-p27mt could inhibit the growth, invasion and metastasis of hepatic cancer by lowering the expressions of VEGF and MMP-9.

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References

  1. Turrell SJ, Whitehouse A. Gene therapy against hepatocellular carcinoma: Development of a herpesvirus saimiri-based vector. Human Gene Ther, 2009,20(4):396–422

    Article  Google Scholar 

  2. Spangenberg HC, Thimme R, Blum HE. Advances in prevention and diagnosis of hepatocellular carcinoma. Expert Rev Gastroenterol Hepatol, 2008,2(3):425–433

    Article  CAS  PubMed  Google Scholar 

  3. Plyk K, Lee MH, Erdjument-bromage H, et al. Cloning of p27kip1,a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic. Cell, 1994,78(1):59–66

    Article  Google Scholar 

  4. Deli G, Jin CH, Mu R, et al. Immunohistochemical assessment of angiogenesis in hepatocellular arcinoma and surrounding cirrhotic liver tissues. World J Gastroenterol, 2005,11(7):960–963

    PubMed  Google Scholar 

  5. Nemec KN, Khaled AR. Therapeutic modulation of apoptosis: targeting the BCL-2 family at the interface of the mitochondrial membrane. Yonsei Med J, 2008, 49(5):689–697

    Article  CAS  PubMed  Google Scholar 

  6. Ellis FH, Xu X, Kulke MH, et al. Malignant transformation of the esophageal mucosa is enhanced in p27 knockout mice. J Thorac Cardiovasc Surg, 2001,122(4):809–814

    Article  PubMed  Google Scholar 

  7. Yang HY, Shao R, Hung MC, et al. p27 Kip1 inhibits HER2/neu-mediated cell growth and tumorigenesis. Oncogene, 2001,20(28):3695–3702

    Article  CAS  PubMed  Google Scholar 

  8. Jean A, Hurteau MD, Susan A, et al. Overexpression of a stabilized mutant form of the cyclin-dependent kinase inhibitor p27kip1 inhibits cell growth. Gynecologic Oncology, 2002,86(1):19–23

    Article  Google Scholar 

  9. Liang J, Shao SH, Xu ZX, et al. The energy sensing LKB1-AMPK pathway regulates p27(kip1) phospho-rylation mediating the decision to enter autophagy or apoptosis. Nat Cell Biol, 2007,9(2):218–224

    Article  CAS  PubMed  Google Scholar 

  10. Naruse I, Hoshino H, Dobashi K, et al. Over-expression of p27kip1 induces growth arrest and apoptosis mediated by changes of p27 expression in lung cancer cell lines. Int J Cancer, 2000,88(3):377–383

    Article  CAS  PubMed  Google Scholar 

  11. Chiu CT, Yeh TS, Hsu JC, et al. Expression of Bcl-2 family modulated through p53-dependent pathway in human hepatocellular carcinoma. Dig Dis Sci, 2003,48(4): 670–676

    Article  CAS  PubMed  Google Scholar 

  12. Ravazoula P, Tsamandndas AC, Kardamakis D, et al. The potential role of bcl-2 mRNA and protein expression in hepatocellular carcinoma. Anticancer Res, 2002,22(3): 1799–1805

    CAS  PubMed  Google Scholar 

  13. Wyllie AH. Apoptosis. Death gets a brake. Nature, 1994,369(6478):272–273

    Article  CAS  PubMed  Google Scholar 

  14. Yao Y, Huang C, Li ZF, et al. Exogenous phosphatidyl-ethanolamine induces apoptosis of human hepatoma HepG2 cells via the bcl-2/Bax pathway. World J Gastroenterol, 2009,15(14):1751–1758

    Article  CAS  PubMed  Google Scholar 

  15. Li XL, Xian ZY, Shi RS, et al. The DSA characteristics and the expression of VEGF of primary hepatocellular carcinoma: A correlative study. J Interventional Radiol, 2010,19(5):377–381

    Google Scholar 

  16. Yang P, Yuan W, He J, et al. Overexpression of EphA2, MMP-9, and MVD-CD34 in hepatocellular carcinoma: Implications for tumor progression and prognosis. Hepatol Res, 2009,39(12):1169–1177

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital, Wuhan University, Wuhan, 430060, China

    Xianxiang Chen  (陈先祥) & Weixing Wang  (王卫星)

  2. Department of Hepatobiliary and Pancreatic Surgery, Renmin Hospital, Hubei Medical University, Shiyan, 442000, China

    Xianxiang Chen  (陈先祥), Linfei Zhang  (张林菲), Zhijun He  (何志军), Qinghe Cai  (蔡庆和), Xiaobo Liu  (刘小波), Caitao Cheng  (程彩涛) & Liming Wu  (吴黎明)

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  1. Xianxiang Chen  (陈先祥)
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  2. Weixing Wang  (王卫星)
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  3. Linfei Zhang  (张林菲)
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  4. Zhijun He  (何志军)
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  5. Qinghe Cai  (蔡庆和)
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  6. Xiaobo Liu  (刘小波)
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  7. Caitao Cheng  (程彩涛)
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  8. Liming Wu  (吴黎明)
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Corresponding author

Correspondence to Weixing Wang  (王卫星).

Additional information

This project was supported by a grant form the Program of Scientific Research of Bureau of Science and Technology of Hubei Province, China (No. 2005ABA082).

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Chen, X., Wang, W., Zhang, L. et al. Effects of Ad-p27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 611–614 (2010). https://doi.org/10.1007/s11596-010-0551-0

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  • DOI: https://doi.org/10.1007/s11596-010-0551-0

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