Summary
This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication. In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively. The results showed that, at certain time interval, insulin could down-regulate expression of type I VLDLR and up-regulate the expression of type II VLDLR in SGC7901 cells, at both protein and RNA level. We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.
Similar content being viewed by others
References
Bujo H, Hermann M, Kaderli MO, et al. Chicken oocyte growth is mediated by an eight ligand binding repeat member of the LDL receptor family. EMBO J, 1994,13(21):5165–5175
Takahashi S, Sakai J, Fujino T, et al. The very low density lipoprotein (VLDL) receptor — A peripheral lipoprotein receptor for remnant lipoproteins into fatty acid active tissues. Mol Cell Biochem, 2003,248(1–2):121–127
Eck MV, Oost J, Goudriaan JR, et al. Role of the macrophage very-low-density lipoprotein receptor in atherosclerotic lesion development. Atherosclerosis, 2005,183(2):230–237
Kamagate A, Qu S, Perdomo G, et al. FoxO1 mediates insulin-dependent regulation of hepatic VLDL production in mice. J Clin Invest, 2008,118(6):2347–2364
Grefhorst A, Hoekstra J, Derks TG, et al. Acute hepatic steatosis in mice by blocking beta-oxidation does not reduce insulin sensitivity of very low density lipoprotein production. Am J Physiol Gastrointest Liver Physiol, 2005,289(3):G592–G598
Wittmaack FM, Gåfvels ME, Bronner M, et al. Localization and regulation of the human very low density lipoprotein/apolipoprotein-E receptor trophoblast expression predicts a role for the receptor in placental lipid transport. Endocrinology, 1995,136(1):340–348
Takahashi S, Kawarabayashi Y, Nakai T, et al. Rabbit very low-density lipoprotein receptor: a low-density lipoprotein receptor like protein with distinct ligand specificity. Proc Natl Acad Sci USA, 1992,89(19):9252–9256
Austin MA. Plasma triglyceride and coronary heart disease. Arterioscler Thromb, 1991,11(1):2–14
Albalat A, Gutierrez J, Navarro I. Regulation of lipolysis in isolated adipocytes of rainbow trout (Oncorhynchus mykiss): the role of insulin and glucagons. Comp Biochem Physiol A Mol Integr Physiol, 2005,142(3):347–354
Albalat A, Saera-Vila A, Capilla E, et al. Insulin regulation of lipoprotein lipase (LPL) activity and expression in gilthead sea bream (Sparus aurata). Comp Biochem Physiol A Mol Integr Physiol, 2007,148(2): 151–159
Jokinen EV, Landschulz KT, Wyne KL, et al. Regulation of the very low-density lipoprotein receptor by thyroid hormone in rat skeletal muscle. J Biol Chem, 1994, 269(42):26411–26418
Li YH, Liu ZG, Chen T, et al. Difference of lipoprotein binding and internalization between two isoforms of very low density lipoprotein receptor. J Huazhong Univ Sci Technol [Med Sci], 2005,35(2):141–145
Yang L, Tian J, Liu ZG, et al. Effect of statins on the expression of two VLDL receptor subtypes. J Huazhong Univ Sci Technolog [Med Sci], 2007,36(4):437–440
Kosaka S, Takahashi S, Masamura K, et al. Evidence of macrophage foam cell formation by very low-density lipoprotein receptor: interferon-gamma inhibition of very low-density lipoprotein receptor expression and foam cell formation in macrophages. Circulation, 2001,103(8): 1142–1147
Stollenwerk MM, Schiopu A, Fredrikson GN, et al. Very low density lipoprotein potentiates tumor necrosis factor-alpha expression in macrophages. Atherosclerosis, 2005,179(2):247–254
Takahashi M, Takahashi S, Suzuki C, et al. Interleukin-1β attenuates β-very-low-density lipoprotein uptake and its receptor expression in vascular smooth muscle cells. J Mol Cell Cardiol, 2005,38(4):637–646
Pollak M. Targeting insulin and insulin-like growth factor signalling in Oncology. Curr Opin Pharmacol, 2008,8(4):384–392
Frasca F, Pandini G, Malaguarnera R, et al. Role of c-Abl in directing metabolic versus mitogenic effects in insulin receptor signaling. J Biol Chem, 2007,282(36): 26077–26088
Yang P, Liu Z, Wang H, et al. Enhanced activity of very low density lipoprotein receptor II promotes SGC7901 cell proliferation and migration. Life Sciences, 2009, 84(13–14):402–408
Adiels M, Westerbacka J, Soro-Paavonen A, et al. Acute suppression of VLDL1 secretion rate by insulin is associated with hepatic fat content and insulin resistance. Diabetologia, 2007,50(11):2356–2365
Author information
Authors and Affiliations
Corresponding author
Additional information
The two authors contributed equally to this work.
This project was supported by grants from National Natural Sciences Foundation of China (No. 39970307) and Hubei Provincial Natural Sciences Foundation of China (No. 2005ABA092).
Rights and permissions
About this article
Cite this article
Cai, Z., Li, F., Peng, C. et al. Effect of insulin on the differential expression of VLDL receptor isoforms of SGC7901 cell and its biological implication. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 551–555 (2010). https://doi.org/10.1007/s11596-010-0541-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-010-0541-2