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Quantitative analysis of cell tracing by in vivo imaging system

Journal of Huazhong University of Science and Technology [Medical Sciences] volume 30, pages 541–545 (2010)Cite this article

Summary

In vivo imaging system (IVIS) is a new and rapidly expanding technology, which has a wide range of applications in life science such as cell tracing. By counting the number of photons emitted from a specimen, IVIS can quantify biological events such as tumor growth. We used B16F10-luc-G5 tumor cells and 20 Babl/C mice injected subcutaneously with B16F10-luc-G5 tumor cells (1×106 in 100 μL) to develop a method to quantitatively analyze cells traced by IVIS in vitro and in vivo, respectively. The results showed a strong correlation between the number of tumor cells and the intensity of bioluminescence signal (R2=0.99) under different exposure conditions in in vitro assay. The results derived from the in vivo experiments showed that tumor luminescence was observed in all mice by IVIS at all days, and there was significant difference (P<0.01) between every two days from day 3 to day 14. Moreover, tumor dynamic morphology could be monitored by IVIS when it was invisible. There was a strong correlation between tumor volume and bioluminescence signal (R2=0.97) by IVIS. In summary, we demonstrated a way to accurately carry out the quantitative analysis of cells using IVIS both in vitro and in vivo. The data indicate that IVIS can be used as an effective and quantitative method for cell tracing both in vitro and in vivo.

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Affiliations

  1. Instiute of Organ Transplantation, Key Lab of Ministry of Education/Ministry of Health of China, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Junmeng Zheng, Lijun Xu, Hongmin Zhou, Weina Zhang & Zhonghua Chen

  2. Department of Cardiothoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Lijun Xu & Hongmin Zhou

Authors
  1. Junmeng Zheng
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  2. Lijun Xu
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  3. Hongmin Zhou
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  4. Weina Zhang
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  5. Zhonghua Chen
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Corresponding author

Correspondence to Zhonghua Chen.

Additional information

These authors contributed equally to this work.

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30901364), and grants from the National Basic Research Program of China (No. 2003CB515505, 2009CB522407).

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Zheng, J., Xu, L., Zhou, H. et al. Quantitative analysis of cell tracing by in vivo imaging system. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 541–545 (2010). https://doi.org/10.1007/s11596-010-0465-x

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  • DOI: https://doi.org/10.1007/s11596-010-0465-x

Key words

  • in vivo imaging system
  • cell tracing
  • quantitative analysis