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Blockade of γc signals in combination with donor-specific transfusion induces cardiac allograft acceptance in murine models

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Summary

The γc cytokines play an important role in proliferation and survival of T cells. Blocking the γc signals can cause the activated donor-reactive T cells losing the ability to proliferate, and getting into apoptosis pathway, which contributes to induction of the peripheral tolerance. In this study, we induced the transplant tolerance through blocking the γc in combination with donor-specific transfusion (DST) in the cardiac transplantation. Following DST, on the day 2, 4 and 6, C57BL/6 recipients received anti-γc monoclonal antibodies (mAbs) injection, and those in control group were not given anti-γc mAbs. On the day 7, Balb/c cardiac allografts were transplanted. All recipients in experimental group accepted cardiac allografts over 30 days, and two of them accepted allografts without rejection until sacrifice on the 120 day. Animals only receiving DST rejected grafts within 5 days, and the mice receiving cardiac transplantation alone rejected grafts within 9 days. Our study showed that blockade of γc signaling combined with DST significantly prolonged allograft survival, which was probably associated with inhibition of antigen-specific T-cell proliferation and induction of apoptosis.

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Authors and Affiliations

  1. Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Sheng Chang  (昌 盛), Xingguang Lin  (林星光), Fuli Xiang  (向芙莉) & Zhonghua Chen  (陈忠华)

  2. Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Li Wang  (汪 理)

  3. Wenzhou Key Laboratory of Surgery, Zhejiang Provincial Top Key Discipline in Surgery, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China

    Bicheng Chen  (陈必成)

Authors
  1. Sheng Chang  (昌 盛)
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  2. Li Wang  (汪 理)
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  3. Xingguang Lin  (林星光)
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  4. Fuli Xiang  (向芙莉)
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  5. Bicheng Chen  (陈必成)
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  6. Zhonghua Chen  (陈忠华)
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Corresponding author

Correspondence to Li Wang  (汪 理).

Additional information

This project was supported by grants from the National Natural Sciences Foundation of China (No. 30500468 and 30700768).

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Chang, S., Wang, L., Lin, X. et al. Blockade of γc signals in combination with donor-specific transfusion induces cardiac allograft acceptance in murine models. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 421–424 (2010). https://doi.org/10.1007/s11596-010-0442-4

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  • DOI: https://doi.org/10.1007/s11596-010-0442-4

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