Summary
To examine the changes in matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in the development and progression of endometriosis, real time quantitative polymerase chain reaction, enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21st day after the induction in mice with induced endometriosis (experimental group) and sham-operated animals (controls). The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group. MMP-9 had two or three peaks during the 21 days, taking place at day 1, 4 and 15. It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.
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This project was supported by grants from Xiamen Municipal Scientific Research Program (No. 3502 Z 20077047), fund for Medical Research Projects of Scientific and Technological Program (No. 3502 Z 20084006) and a medical innovation-supporting program of Fujian Province (No. 2009-CXB-49).
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Chen, Q., Qiu, N., Pu, D. et al. Change profiles in matrix metalloproteinase-2 and -9 in induced endometriosis in mice. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 188–192 (2010). https://doi.org/10.1007/s11596-010-0211-4
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DOI: https://doi.org/10.1007/s11596-010-0211-4