Skip to main content
SpringerLink
Log in

The preparation and cytocompatibility of injectable thermosensitive chitosan/poly(vinyl alcohol) hydrogel

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

In order to investigate the strength, structure and cell cytocompatibility of injectable thermosensitive chitosan (CS)/poly(vinyl alcohol) (PVA) composite hydrogel, chitosan hydrochloride solution was transferred to a neutral pH and mixed with different proportions of PVA, then the gelation time and strength of these different hydrogels were tested and spatial structures were observed under a scanning electron microscopy (SEM) after freeze-drying. The cytocompatibility of the hydrogels was evaluated through cytotoxicity test and three-dimensional culture with bone marrow mesenchymal stem cells. The results showed that the CS/PVA solution kept in liquid state at low temperature (0–4°C) and turned into transparent elastomer about 15–20 min at 37°C. Gelation time was prolonged, the strength increased and porous structure became dense with the PVA content increased in the mixed hydrogel. The cytotoxicity grades of these gels were from 0 to 1. Rabbit bone marrow mesenchymal stem cells could survive and proliferate in the gel within 3 weeks, and the gel had good cytocompatibility. It was concluded that thermosensitive CS/PVA composite hydrogel not only has interpenetrating network structure and better mechanical strength, but also has good cytocompatibility, and may be used as an injectable scaffold for tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kim IY, Seo SJ, Moon HS, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnol Adv, 2008,26(1):1–21

    Article  CAS  PubMed  Google Scholar 

  2. Kumar MN, Muzzarelli RA, Muzzarelli C, et al. Chitosan chemistry and pharmaceutical perspectives. Chem Rev, 2004,104(12):6017–6084

    Article  PubMed  Google Scholar 

  3. Khor E, Lim LY. Implantable applications of chitin and chitosan. Biomaterials, 2003,24(13):2339–2349

    Article  CAS  PubMed  Google Scholar 

  4. Sezer AD, Cevher E, Hatipoǧlu F, et al. Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits. Biol Pharm Bull, 2008,31(12):2326–2333

    Article  CAS  PubMed  Google Scholar 

  5. Gutowska A, Jeong B, Jasionowski M. Injectable gels for tissue engineering. Anat Rec, 2001,263(4):342–349

    Article  CAS  PubMed  Google Scholar 

  6. Shu XZ, Liu YC, Palumbo FS, et al. In situ crosslinkable hyaluronan hydrogels for tissue engineering. Biomaterials, 2004,25(7–8):1339–1348

    CAS  Google Scholar 

  7. Jeong B, Kim SW, Baeb YH. Thermosensitive sol-gel reversible hydrogels. Adv Drug Deliv Rev, 2002,54(1):37–51

    Article  CAS  PubMed  Google Scholar 

  8. Park J, Kim D. Release behavior of amoxicillin from glycol chitosan superporous hydrogels. J Biomater Sci Polym Ed, 2009,20(5–6):853–862

    Article  CAS  PubMed  Google Scholar 

  9. de Souza Costa-Júnior E, Pereira MM, Mansur HS. Properties and biocompatibility of chitosan films modified by blending with PVA and chemically crosslinked. J Mater Sci Mater Med, 2009,20(2):553–561

    Article  PubMed  Google Scholar 

  10. Mori Y, Tokura H, Yoshikawa M. Properties of hydrogels synthesized by freezing and thawing aqueous polyvinyl alcohol solutions and their applications. J Materials Sci, 1997,32(2):491–496

    Article  CAS  Google Scholar 

  11. Kurkuri MD, Aminabhavi TM. Poly(vinyl alcohol) and poly(acrylic acid) sequential interpenetrating network pH-sensitive microspheres for the delivery of diclofenac sodium to the intestine. J Control Release, 2004,96(1):9–20

    Article  CAS  PubMed  Google Scholar 

  12. Park H, Kim D. Swelling and mechanical properties of glycolchitosan/poly(vinyl alcohol) IPN-type superporous hydrogels. J Biomed Mater Res A, 2006,78(4):662–667

    PubMed  Google Scholar 

  13. Tang Y, Du Y, Li Y, et al. A thermosensitive chitosan/ poly(vinyl alcohol) hydrogel containing hydroxyapatite for protein delivery. J Biomed Mater Res A, 2009,91(4):953–963

    PubMed  Google Scholar 

  14. Gong Z, Ding SS, Yin YJ, et al. Optimized design of hy drogels for tissue engineering. Hua Xue Gongye Yu Gong Cheng Jin Zhan (Chinese). 2008,27(11):1743–1748

    CAS  Google Scholar 

  15. Bureau of Technical Supervision. The People’s Republic of China National Standards: Biological evaluation of medical silicone materials test methods (GB/T 16175-1996)[S]. Beijing: China Standard Press, 1996:6,17–18

    Google Scholar 

  16. Kretlow JD, Klouda L, Mikos AG. Injectable matrices and scaffolds for drug delivery in tissue engineering. Adv Drug Deliv Rev, 2007,59(4–5):236–273

    Google Scholar 

  17. Balakrishnan B, Jayakrishnan A. Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds. Biomaterials, 2005,26(18):3941–3951

    Article  CAS  PubMed  Google Scholar 

  18. Chenite A, Chaput C, Wang D, et al. Novel injectable neutral solutions of chitosan form biodegradable gels in situ. Biomaterials, 2000,21(21):2155–2161

    Article  CAS  PubMed  Google Scholar 

  19. Cui YL, Qi AD, Liu WG, et al. Biomimetic surface modification of poly(L-lactic acid) with chitosan and its effects on articular chondrocytes in vitro. Biomaterials, 2003,24(21):3859–3868

    Article  CAS  PubMed  Google Scholar 

  20. Donati I, Stredanska S, Silvestrini G, et al. The aggregation of pig articular chondrocyte and synthesis of extracellular matrix by a lactose-modified chitosan. Biomaterials, 2005,26(9):987–998

    Article  CAS  PubMed  Google Scholar 

  21. Hong Y, Song H, Gong Y, et al. Covalentlycrosslinked chitosan hydrogel: properties of in vitro degradation and chondrocyte encapsulation. Acta Biomaterialia, 2007,3(1): 23–31

    Article  CAS  PubMed  Google Scholar 

  22. Bhattarai N, Ramay HR, Gunn J, et al. PEG-grafted chitosan as an injectable thermosensitive hydrogel for sustained protein release. J Control Release, 2005,103(3):609–624

    Article  CAS  PubMed  Google Scholar 

  23. Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials, 2003,24(24):4337–4351

    Article  CAS  PubMed  Google Scholar 

  24. Tan H, Gong Y, Lao L, et al. Gelatin/chitosan/hyaluronan ternary complex scaffold containing basic fibroblast growth factor for cartilage tissue engineering. J Mater Sci Mater Med, 2007,18(10):1961–1968

    Article  CAS  PubMed  Google Scholar 

  25. Crompton KE, Goud JD, Bellamkonda RV, et al. Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering. Biomaterials,2007,28(3):441–449

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microorthopaedics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Baiwen Qi  (漆白文), Aixi Yu  (喻爱喜), Shaobo Zhu  (祝少博) & Biao Chen  (陈 彪)

  2. College of Resource and Environmental Science of Wuhan University, Wuhan, 430079, China

    Yan Li  (李 艳)

Authors
  1. Baiwen Qi  (漆白文)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aixi Yu  (喻爱喜)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaobo Zhu  (祝少博)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Biao Chen  (陈 彪)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan Li  (李 艳)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aixi Yu  (喻爱喜).

Additional information

This project was supported by grants from National Natural Sciences Foundation of China (No. 30770574) and Research Fund Project of Health Department of Hubei Province (No. JX3B21).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qi, B., Yu, A., Zhu, S. et al. The preparation and cytocompatibility of injectable thermosensitive chitosan/poly(vinyl alcohol) hydrogel. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 89–93 (2010). https://doi.org/10.1007/s11596-010-0116-2

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-010-0116-2

Key words

Search

Navigation