Summary
This study examined the expressions of human serum tissue factor (TF) and tissue factor pathway inhibitor (TFPI) in patients with acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and their clinical significance. The serum TF and TFPI levels were detected by ELISA in 28 allo-HSCT recipients before and after the transplantation and the changes of TF and TFPI levels were dynamically monitored at different phases of the disease. No significant differences in the serum TF and TFPI levels were found in allo-HSCT recipients in the absence of aGVHD or with grade I aGVHD before and after the transplantation. The levels of serum TF and TFPI were substantially increased in the patients with gradeII aGVHD at the peak of aGVHD (P<0.05) and they were even higher in the patients with grade III–IV aGVHD (P<0.01). When the conditions became stable after treatment with immunosuppressive agents, the serum TFPI level was decreased to the baseline level (P>0.05) and the TF level was lowered but still higher than the baseline level (P<0.05). It was concluded that the levels of serum TF and TFPI were increased significantly in the patients with grade II–IV aGVHD after allo-HSCT and decreased markedly after the treatment. Monitoring the levels of serum TF and TFPI in the patients with allo-HSCT is important to predict the occurrence, outcome and prognosis of aGVHD.
Similar content being viewed by others
References
Hattori K, Hirano T, Yamakawa, et al. Differential effects of anti-Fas ligand and anti TNF-A antibodies on acute graft vs host disease pathologies. Blood, 1998, 91(11):4051–4055
Falanga A, Vignoli A, Marchetti M, et al. Defibrotide reduces procoagulant activity and increases fibronolytic properties of endothelial cells. Leukemia, 2003,17(8):1636–1642
Camerer E, Kolsto AB, Prydz H. Cell biology of tissue factor, the principal initiator of blood coagulation. Thromb Res, 1996,81(1):1–41
Pyo RT, Sato Y, Mackman N, et al. Mice deficient in tissue factor demonstrate attenuated intimal hyperplasia in response to vascular injury and decreased smooth muscle cell migration. Thromb Haemost, 2004,92(3):451–458
Carmeliet P, Mackman N, Moons L, et al. Role of tissue factor in embryonic blood vessel development. Nature, 1996, 383(6595):73–75
Rak J, Milsom C, May L, et al. Tissue factor in cancer and angiogenesis: the molecular link between genetic tumor progression, tumor neovascularization, and cancer coagulopathy. Semin Thromb Hemost, 2006,32(1):54–70
Janin A, Deschaumes C, Daneshpouy M, et al. CD95 engagement induces disseminated endothelial cell apoptosis in vivo: immunopathologic implications. Blood, 2002,99(8):2940–2947
Zhang ZL, Shen T. Blood disease diagnosis and efficacy standards (the third version). Beijing: Science Press, 1999,269–276
Thomas ED, Storb R, Clift RA, et al. Bone marrow transplantation. New Engl J Med, 1975,292(4):832–8434
Sureda A, Schmitz N. Allogeneic stem cell transplantation after reduced-intensity conditioning in lymphoid malignancies. Ann Hematol, 2003,82(1):1–13
Bleakley M, Riddell SR. Molecules and mechanisms of the graft-versus-leukaemia effect. Nat Rev Cancer, 2004, 4(5):371–380
Naji A, Deschaseaux F, Racadot E, et al. Induction of tissue factor expression on human umbilical vein endothelial cells by cell-specific HLA class I antibody. Transplant Proc, 2005,37(6):2892–2893
Ge X, Karrar A, Ericzon BG, et al. Antibodies to liver sinusoidal endothelial cells modulate immune responses in liver transplantation. Transplant Proc, 2005,37(8):3335–3337
Esmon CT, Fukudome K, Mather T, et al. Inflammation, sepsis, and coagulation. Haematologica, 1999,84(3):254–259
Esmon CT. The interactions between inflammation and coagulation. Br J Haematol, 2005,131(4):417–430
Shebuski RJ, Kilgore KS. Role of Inflammatory mediators in thrombogenesis. Pharmacol Exp Ther, 2002, 300(3):729–735
Shama L, Melis E, Hickey MJ, et al. The cytoplasmic domain of tissue factor contributes to leukocyte recruitment and death in endotoxemia. Am J Pathol, 2004, 165(1):331–403
Spek CA. Tissue factor from just one of the coagulation factor to a major player in physiology. Blood Coagul Fibrinolysis, 2004,15(Suppl 1):S3–10
Imamura T. Tissue factor expression at the site of inflammation: a cross-talk between inflammation and the blood coagulation system. Rinsho Byori, 2004,52(4):342–349
Schmid E, Muller TH, Budzinski RM, et al. Lymphocyte adhesion to human endothelial cells induces tissue factor expression via a juxtacrine pathway. Thromb Haemost, 1995,73(3):421–428
Friedl J, Puhlmann M, Bartlett DL, et al. Induction of permeability across endothelial cell monolayers by tumor necrosis factor (TNF) occurs via a tissue factor-dependent mechanism: relationship between the procoagulant and permeability effects of TNF. Blood, 2002,100(4):1334–1339
Levy MM, Fink MP, Marshall JC, et al. 2001 SCCM/ESICM/ACCP/ATS/SI international sepsis definitions conference. Crit Care Med, 2003,31(4):1250–1256
Gando S, Kameue T, Morimoto Y, et al. Tissue factor production not balanced by tissue factor pathway inhibitor in sepsis promotes poor prognosis. Crit Care Med, 2002, 30(8):1729–1734
Enkhbaatar P, Okajima K, Murakami K, et al. Recombinant tissue factor pathway inhibitor reduces lipopolysaccharide induced pulmonary vascular lung injury by inhibiting leukocyte activation. Am J Respir Crit Care Med, 2000,162(5):1752–1759
Hansen JB, Svensson B, Olsen R, et al. Heparin induces synthesis and secretion of tissue factor pathway inhibitor from endothelial cells in vitro. Thromb Haemost, 2000, 83(6):937–943
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hao, J., Li, W., Zou, P. et al. Expressions of tissue factor and tissue factor pathway inhibitor in patients with acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 697–700 (2009). https://doi.org/10.1007/s11596-009-0604-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-009-0604-4