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Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead

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Summary

To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial cells from Wistar rat brain were primarily cultured in medium with acetate Pb. The processes were terminated at different time points. The immunoprecipitation (IP) and Western blotting were used for GRP78 purification and expression and the Pb concentration was determined by employing atomic absorption spectrophotometry (AAS). The localization change of GRP78 was observed with colloid gold immunoelectron microscopy. The results showed that the expression of GRP78 was increased significantly in the cells treated with 1.0 μmol/L acetate Pb for 24 h and peaked at 96–192 h (P<0.01), and at the 12th day, the expression of GRP78 began to decrease but was still higher than normal (P<0.05). Pb content started to increase when cells were treated by acetate Pb for 24 h, and the peak appeared at 8 day (P<0.01), and then Pb content decreased gradually, but was still higher than normal (P<0.05). GRP78 protein expression began to remarkably increase when it transferred from ER to the cytosol around the nuclei 24 h after treatment with Pb. It is concluded that GRP78 in astroglia could strongly chelate with Pb ions and it might be a target protein of Pb.

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Authors and Affiliations

  1. Institute of Neurology, China Medical University, Shenyang, 110001, China

    Ying Zhang  (张 莹)

  2. Department of Pathophysiology, Liaoning Medical University, Jinzhou, 121000, China

    Liping Ye  (叶丽平)

  3. Department of Biochemistry and Molecule Biology, China Medical University, Shenyang, 110001, China

    Biao Wang  (王 彪), Yan Li  (李 妍) & Liguang Sun  (孙黎光)

Authors
  1. Ying Zhang  (张 莹)
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  2. Liping Ye  (叶丽平)
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  3. Biao Wang  (王 彪)
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  4. Yan Li  (李 妍)
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  5. Liguang Sun  (孙黎光)
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Additional information

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 39970651).

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Zhang, Y., Ye, L., Wang, B. et al. Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 492–497 (2009). https://doi.org/10.1007/s11596-009-0420-x

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  • DOI: https://doi.org/10.1007/s11596-009-0420-x

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