Skip to main content
SpringerLink
Log in

Serum vascular endothelial growth factor levels in patients with non-small cell lung cancer and its relations to the micrometastasis in peripheral blood

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

To examine the relationship between the levels of the serum vascular endothelial growth factor (VEGF) and the micrometastasis of peripheral blood in patients with non-small cell lung cancer (NSCLC), 108 NSCLC patients, including 40 patients with benign lung diseases and 30 healthy controls, were investigated. The serum VEGF levels were detected by ELISA and CK19 mRNA in peripheral blood by reverse transcriptase-polymerase chain reaction (RT-PCR). In NSCLC group, the serum VEGF levels and the positive rate of CK19 mRNA in peripheral blood were 479.8±268.5 pg/mL and 66.7%, which were significantly higher than those of the other two groups respectively (P<0.01), and both of them were increased significantly with the progression of clinical stage of the tumors (P<0.01). Serum VEGF levels as well as the positive rate of CK19 mRNA in different pathological types of lung cancer had no significant differences (P>0.05). Serum VEGF levels in the patients positive for CK19 mRNA was 561.7±325.6 pg/mL. It is significantly higher than that in the negative patients (P<0.01). There existed a significant correlation between serum VEGF levels and expression of CK19 mRNA in peripheral blood in NSCLC patients (P<0.001). The detection of serum VEGF levels and CK19 mRNA in peripheral blood is helpful in judging the condition and the prognosis of NSCLC patients, and serum VEGF levels and CK19 mRNA are independent of the pathological types of lung cancer. The micrometastasis in peripheral blood of NSCLC patients is significantly associated with serum VEGF levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Osaki T, Oyama T, Inoue M, et al. Molecular biological markers, an micrometastasis in resected non-small cell lung cancer. Prognostic implications. Jap J Thorac Cardiovasc Surg, 2001,49(9):545–541

    CAS  Google Scholar 

  2. Jiao X, Krasna MJ. Clinical significance of micrometastasis in lung and esophageal cancer: a new paradigm in thoracic oncology. Ann Thorac Surg, 2002,74(1):278–284

    Article  PubMed  Google Scholar 

  3. Le Pimpec-Barthes F, Danel C, Lacave R, et al. Association of CK19 mRNA detection of occult cancer cells in mediastinal lymph nodes in non-small cell lung carcinoma and high risk of early recurrence. Eur J Cancer, 2005, 41(2):306–312

    Article  PubMed  CAS  Google Scholar 

  4. Buccheri G, Ferrigno D. Cytokeratin-derived markers of lung cancer. Expert Rev Mol Diagn, 2001,1(3):315–322

    Article  PubMed  CAS  Google Scholar 

  5. Takahashi Y, Kitadai Y, Bucana CD, et al. Expression of vascular endothelial growth factor and its receptor, KDR, correlates with vascularity, metastasis and proliferation of human colon cancer. Cancer Res, 1995,55(18):3964–3968

    PubMed  CAS  Google Scholar 

  6. Dvorak HF, Nagy JA, Feng D, et al. Vascular permeability factor/vascular endothelial growth factor and the significance of microvascular hyperpermeability in angiogenesis. Curr Top Microbiol Immunol, 1999,237:97–132

    PubMed  CAS  Google Scholar 

  7. Folkman J. What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst, 1990,82(1):4–6

    Article  PubMed  CAS  Google Scholar 

  8. Qu Z, Van Ginkel S, Roy AM, et al. Vascular endothelial growth factor reduces tamoxifen efficacy and promotes metastatic colonization and desmoplasia in breast tumors. Cancer Res, 2008,68(15):6232–6240

    Article  PubMed  CAS  Google Scholar 

  9. Shibuya M. Vascular endothelial growth factor-dependent and -independent regulation of angiogenesis. BMB Rep, 2008,41(4):278–286

    PubMed  CAS  Google Scholar 

  10. Hosch SB, Scheunemann P, Izbicki JR. Minimal residual disease in non-small-cell lung cancer. Semin Surg Oncol, 2001,20(4):278–281

    Article  PubMed  CAS  Google Scholar 

  11. Saad AG, Yeap BY, Thunnissen FB, et al. Immunohistochemical markers associated with brain metastases in patients with nonsmall cell lung carcinoma. Cancer, 2008, 113(8):2129–2138

    Article  PubMed  Google Scholar 

  12. Achen MG, Stacker SA. Molecular control of lymphatic metastasis. Ann N Y Acad Sci, 2008,1131:225–234

    Article  PubMed  CAS  Google Scholar 

  13. Demirkazik A, Akbulut H, Ulger AF, et al. Serum vascular endothelial growth factor levels in patients with non-small cell lung cancer. Ann Saudi Med, 2004,24(6):473–475

    PubMed  Google Scholar 

  14. Futami R, Miyashita M, Nomura T, et al. Increased serum vascular endothelial growth factor following major surgical injury. J Nippon Med Sch, 2007,74(3):223–229

    Article  PubMed  CAS  Google Scholar 

  15. Aita M, Fasola G, Defferrari C, et al. Targeting the VEGF pathway: antiangiogenic strategies in the treatment of non-small cell lung cancer. Crit Rev Oncol Hematol, 2008,68(3):183–196

    Article  PubMed  Google Scholar 

  16. Katsabeki-Katsafli A, Kerenidi T, Kostikas K, et al. Serum vascular endothelial growth factor is related to systemic oxidative stress in patients with lung cancer. Lung Cancer, 2008,60(2):271–276

    Article  PubMed  CAS  Google Scholar 

  17. Holdenrieder S, Stieber P, von Pawel J, et al. Circulating nucleosomes predict the response to chemotherapy in patients with advanced non-small cell lung cancer. Clin Cancer Res, 2004,10(18 Pt 1):5981–5987

    Article  PubMed  CAS  Google Scholar 

  18. Cheng M, Chen Y, Yu X, et al. Diagnostic utility of LunX mRNA in peripheral blood and pleural fluid in patients with primary non-small cell lung cancer. BMC Cancer, 2008,8:156

    Article  PubMed  Google Scholar 

  19. Ge MJ, Wu QC, Wang M, et al. Detection of disseminated lung cancer cells in regional lymph nodes by assay of CK19 reverse transcriptase polymerase chain reaction and its clinical significance. J Cancer Res Clin Oncol, 2005, 131(10):662–668

    Article  PubMed  CAS  Google Scholar 

  20. Saintgny P, Coulon S, Kambouchner M, et al. Real-time RT-PCR detection of CK19, CK7 and MUC1 mRNA for diagnosis of lymph node micrometastases in non small cell lung carcinoma. Int J Cancer, 2005,115(5):777–782

    Article  Google Scholar 

  21. Kasimir-Bauer S, Schleucher N, Weber R, et al. Evaluation of different markers in non-small cell lung cancer: prognostic value of clinical staging, tumour cell detection and tumour marker analysis for tumour progression and overall survival. Oncol Rep, 2003,10(2):475–482

    PubMed  Google Scholar 

  22. Huang C, Liu D, Masuya D, et al. Clinical application of biological markers for treatments of resectable non-small-cell lung cancers. Br J Cancer, 2005,92(7): 1231–1239

    Article  PubMed  CAS  Google Scholar 

  23. Dong Q, Feng J, Huang J, et al. Vascular endothelial growth factor promotes hematogenous metastasis of cancer cells in patients with non-small cell lung cancer. Zhonghua Zhong Liu Za Zhi (Chinese), 2002,24(2): 142–146

    CAS  Google Scholar 

  24. Nishi M, Abe Y, Tomii Y, et al. Cell binding isoforms of vascular endothelial growth factor-A (VEGF189) contribute to blood flow-distant metastasis of pulmonary adenocarcinoma. Int J Oncol, 2005,26(6):1517–1524

    PubMed  CAS  Google Scholar 

  25. Brueckl WM, Schoeberl A, Wirtz RM, et al. Increased vascular-endothelial growth factor (VEGF) tumor expression and response to epidermal growth factor receptor (EGF-R) inhibitor erlotinib in non-small cell lung cancer (NSCLC). J Thorac Oncol, 2008,3(3):314–316

    Article  PubMed  Google Scholar 

  26. Keedy VL, Sandler AB. Inhibition of angiogenesis in the treatment of non-small cell lung cancer. Cancer Sci, 2007, 98(12):1825–1830

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pulmonary Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Yang Jin  (金 阳), Xianzhi Xiong  (熊先智), Yuan Su  (苏 远), Jianwu Hu  (胡建武) & Xiaonan Tao  (陶晓南)

Authors
  1. Yang Jin  (金 阳)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianzhi Xiong  (熊先智)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuan Su  (苏 远)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianwu Hu  (胡建武)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaonan Tao  (陶晓南)
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This project was supported by a grant from Scientific Research Foundation of Hubei Health Bureau of PR China (No. 2005JX2B18).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, Y., Xiong, X., Su, Y. et al. Serum vascular endothelial growth factor levels in patients with non-small cell lung cancer and its relations to the micrometastasis in peripheral blood. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 462–465 (2009). https://doi.org/10.1007/s11596-009-0414-8

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-009-0414-8

Key words

Search

Navigation