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The effects of HBx gene on the expression of DNA repair enzymes hOGG1 and hMYHα mRNA in HepG2 cells

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Summary

To observe the alteration in the expression of DNA repair enzymes hOGG1 and hMYHα and the change in 8-OHdG levels in the HBx gene-transfected cells HepG2/HBx and to explore the mechanisms of the HBV-associated hepatocellular carcinoma, the gene-transfected cells HepG2/HBx which stably expressed HBx was established, and the effect of HBx on the cell cycle and proliferation of HepG2 was examined. By using the β-actin as the interior control, real-time polymerase chain reaction (Real-time qPCR) was employed to quantitatively detect the expression of DNA repair enzymes hOGG1 and hMYHα in the HepG2/HBx, the control cells HepG2 and HepG2 transfected with pcDNA3.1 vector (HepG2/pDNA3.1). The 8-OHdG levels were determined by HPLC/ECD in the established gene-transfected cells HepG2/HBx and the control cells HepG2 and HepG2/pcDNA3.1. Our results showed that the expression of DNA repair enzyme hMYHα in the HepG2/HBx (0.021±0.007) was significantly lower than that of HepG2 (0.099±0.041) (P<0.05) and HepG2/pDNA3.1 (0.121±0.005) (P<0.05). However, the no significant differences existed in the expression of DNA repair enzyme hOGG1 among the three cell strains (P>0.05). The 8-OHdG level in the HepG2/HBx was significantly higher than that in HepG2 and HepG2/pcDNA3.1 (P<0.05). It is concluded that HBx gene may inhibit the expression of DNA repair enzyme hMYHα mRNA to impair the ability to repair the intracellular DNA oxidative damage, to increase the oxidative DNA-adduct 8-OHdG and to affect the nucleotide excision repair function, thus participate in the occurrence and development of hepatocellular carcinoma.

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Bin Cheng  (程 斌), Xiaorong Guo  (郭晓榕), Yaochu Zheng  (郑要初), Ying Wang  (王 颖), Chunyan Liu  (刘春艳) & Peiyuan Li  (黎培员)

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  1. Bin Cheng  (程 斌)
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  2. Xiaorong Guo  (郭晓榕)
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  3. Yaochu Zheng  (郑要初)
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  4. Ying Wang  (王 颖)
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  5. Chunyan Liu  (刘春艳)
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  6. Peiyuan Li  (黎培员)
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Correspondence to Bin Cheng  (程 斌).

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This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30570821).

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Cheng, B., Guo, X., Zheng, Y. et al. The effects of HBx gene on the expression of DNA repair enzymes hOGG1 and hMYHα mRNA in HepG2 cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 187–192 (2009). https://doi.org/10.1007/s11596-009-0210-5

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  • DOI: https://doi.org/10.1007/s11596-009-0210-5

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