Summary
In this study, the expression of IL-24 at maternal-fetal interface and the roles in extravillous trophoblast (the TEV-1 cell line) invasion were examined. Immunohistochemistry was used to detect the expression of IL-24 in villi and decidual tissue. The proliferation of TEV-1 cells under the effect of IL-24 was measured by MTT assay. The invasiveness of TEV-1 cells under the effect of recombinant IL-24 (rhIL-24) was examined by transwell system. Immunohistochemical detection showed that IL-24 was expressed in the villi and decidual tissue, and distributed in villous column, trophoblasts, stroma and blood vessels. The proliferation of TEV-1 cells was not inhibited by rhIL-24 of various concentrations. The examination of invasion in vitro showed that rhIL-24 could inhibit the invasion of TEV-1 cells in a concentration-dependent manner. The results suggested IL-24 could inhibit the invasion of TEV-1 cells. Therefore, IL-24 produced by maternal-fetal interface in human first trimester pregnancy may influence the invasion of trophoblasts and is involved in normal pregnancy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nagamatsu T, Fujii T, Kusumi M et al. Cytotrophoblasts up-regulate soluble fms-like tyrosine kinase-1 expression under reduced oxygen: an implication for the placental vascular development and the pathophysiology of preeclampsia. Endocrinology, 2004,145(11):4838–4845
Jiang H, Lin J J, Su Z Z et al. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene, 1995,11: 2477–2486
Saeki T, Mhashikar A, Swanson X et al. Inhibition of human lung cancer growth following adenovirus-mediated mda-7 gene expression in vivo. Oncogene, 2002,21(29): 4558–4566
Sarkar D, Su Z Z, Lebedeva I V et al. Mda-7 (IL-24) mediates selective apoptosis in human melanoma cells by inducing the coordinated overexpression of the GADD family of genes by means of p38 MAPK. Proc Natl Acad Sci USA, 2002,99(15):10054–10059
Feng H C, Choy M Y, Deng W et al. Establishment and characterization of a human first-trimester extravillous trophoblast cell line (TVE-1). J SOC Gynecol Investing, 2005,12(4):e21–e32
Das C, Kumar V S, Gupta S et al. Network of cytokines, integrins and hormones in human trophoblast cells. J Reprod Immunol, 2002,53(1–2):257–268
Caudell E G, Mumm J B, Poindexter N et al. The protein product of the tumor suppressor gene, melanoma differentiation associated gene 7, exhibits immunostimulatory activity and is designated IL-24. J Immunol, 2002, 168(12):6041–6046
Mhashilkar, A M, Schrock R D, Hindi M et al. Melanoma differentiation associated gene-7 (mda-7): A novel anti-tumor gene for cancer gene therapy. Mol Med, 2001, 7(4):271–282
Lebedeva I V, Su Z Z, Chang Y et al. The cancer growth suppressing gene mda-7 induces apoptosis selectively in human melanoma cells. Oncogene, 2002,21(5):708–718
Lebedeva I V, Su Z Z, Sarkar D et al. Melanoma differentiation associated gene-7, mda-7/interleukin 24, induces apoptosis in prostate cancer cells by promoting mitochondrial dysfunction and inducing reactive oxygen species. Cancer Res, 2003,63(23):8138–8144
Pataer A, Vorburger Sa, Barber G N et al. Adenovir transfer of the melanoma differentiation associated gene-7 (mda-7) induces apoptosis of lung cancer cells via up-regulation of the double-stranded RNA-dependent protein kinase (PKR). Cancer Res, 2002,62(8):2239–2243
Su Z Z, Madireddi M T, Lin J J et al. The cancer growth suppressor gene mda-7 selectively induces apoptosis in human breast cancer cells and inhibits tumor growth in nude mice. Proc Natl Acad Sci USA, 1998,95(24): 14400–14409
Ramesh R, Mhashilkar A M, Tanaka F et al. Melanoma differentiation associated gene 7/Interleukin (IL)-24 is a novel ligand that regulates angiogenesis via the IL-22 receptor. Cancer Res, 2003,63(16):5105–5113
Ellerhorst J A, Prieto V G, Ekmekcioglu S et al. Loss of MDA-7 expression with progression of melanoma. J Clin Oncol, 2002,20(4):1069–1074
Ramesh R, Ito I, Gopalan B et al. Ectopic production of mda-7/IL-24 inhibits invasion and migration of human lung cancer cells. Mol Ther, 2004,9(4):510–518
Shi H, Wei L L, Yuan C F et al. Melanoma differentiation-associated gene-7/interleukin 24 inhibits invasion and migration of human cervical cancer cells in vitro. Saudi Med J, 2007,28(11):1671–1675
Kliman H J. Uteroplacental blood flow. The story of decidualization, menstruation and trophoblast invasion. Am J Pathol, 2000,157(6):1759–1768
Bilban M, Head S, Desoye G et al. DNA microarray: a novel approach to investigate genomics in trophoblast invasion—a review. Placenta, 2000,21:99–105
Goldman-Wohl D, Yagel S. Regulation of trophoblast invasion: from normal implantation to preeclampsia. Mol Cell Endocrinol, 2002,187(1–2):233–238
Cohen M, Bischof P. Factors regulating trophoblast invasion. Gynecol Obstet Invest, 2007,64(3):126–130
Bischof P, Meisser A, Campana A. Control of MMP-9 expression at the maternal-fetal interface. J Reprod Immunol, 2002,55(1–2):3–10
Herrler A, von Rango U, Beier H M. Embryo-maternal signalling: how the embryo starts talking to its mother to accomplish implantaion. Reprod Biomed Online, 2003, 6(2):244–256
Author information
Authors and Affiliations
Additional information
Hong CHENG, female, born in 1966, M.D., Ph.D.
This project was supported by a grant from the Natural Science Foundation of Hubei province (No. 2005ABA149).
Rights and permissions
About this article
Cite this article
Cheng, H., Zou, L. IL-24 expression at maternal-fetal interface and its roles in trophoblast invasion. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 456–459 (2008). https://doi.org/10.1007/s11596-008-0418-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-008-0418-9