Summary
The effects of lutein on the growth and migration of bovine lens epithelial cells (BLECs) in vitro were observed in an attempt to find a drug that can prevent after-cataract. BLECs were cultured in vitro and different concentrations of lutein were added to the BLECs cultures of the second and third generations. The effects of lutein on the proliferation of BLECs in vitro were examined by the MTT method, and the migration of BLECs was evaluated by a scratch wound assay. The results showed that: (1) Lutein at concentrations of 1 to 16 μmol/L could inhibit the proliferation of BLECs in a dose-and time-dependent manner (P<0.01): (2) The migration of BLECs was evaluated by wound healing rate. As compared with the control group, the wound healing rate in the experimental groups was decreased from 0.672±0.164 to −0.234±0.144 and −0.597±0.063 (P<0.01) at 1 and 2 μmol/L lutein, respectively. It was concluded that lutein at concentration of ≥1 μmol/L inhibited the proliferation and migration of BLECs in vitro. Lutein may become an effective drug to prevent after-cataract.
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References
McDevitt T M, Tchao R, Harrison E H et al. Carotenoids normally present in serum inhibit proliferation and induce differentiation of a human monocyte/macrophage cell line (U937). Nutr, 2005,135(2):160–164
Zhang M, Zhang X F, Gao X D et al. Drug effects on lens epithlial cell proliferation using the MTT colorimetric assay. Chin Ophthal Res, 1997,15(4)233–235
Liu T, Jin Y, Wang X W et al. Effect of available components in culture supernatants of fibroblasts on fibroblasts. Chin J Conserv Dent, 2006,16(3):129
Itagaki S, Ogura W, Sato Y et al. Characterization of the disposition of lutein after i.v. administration to rats. Boil Pharm Bull, 2006,29(10):2123–2125
Santosa S, Jones P J. Oxidative stress in ocular disease: Does lutein play a protective role? CMAJ, 2005,11; 173(8):861–862
Wang E, Zhao M, Forrester J V et al. Electric Fields and MAP kinase signaling can regulate early wound healing in lens epithelium. Invest Ophthalmol Vis Sci, 2003,44(1): 244–249
Chew B P, Brown C M, Park J S et al. Dietary lutein inhibits mouse mammary tumor growth by regulating angiogenesis and apoptosis. Anticancer Res, 2003,23(4):3333–3339
Gross M D, Bishop T D, Belcher J D et al. Induction of HL-60 cell differentiation by carotenoids. Nutr Cancer, 1997,27(2):169–173
Lee D K, Grantham R N, Mannion J D et al. Carotenoids enhance phosphorylation of Akt and suppress tissue factor activity in human endothelial cells. Nutr Biochem, 2006,17(11):780–786
Selvaraj R K, Klasing K C. Lutein and eicosapentaenoic acid interact to modify iNOS mRNA levels through the PPARgamma/RXR pathway in chickens and HD11 cell lines. Nutr, 2006,136(6):1610–1616
Jin X H, Ohgami K, Shiratori K et al. Inhibitory effects of lutein on endotoxin-induced uveitis in Lewis rats. Invest Ophthalmol Vis Sci, 2006,47(6):2562–2568
Chew B P, Wong M W, Wong T S. Effects of lutein from marigold extract on immunity and growth of mammary tumors in mice. Anticancer Res, 1996,16(6B):3689–3694
Chang S, Erdman J W Jr, Clinton S K et al. Relationship between plasma carotenoids and prostate cancer. Nutr Cancer, 2005,53(2):127–134
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Hu, Y., Xu, Z. Effects of lutein on the growth and migration of bovine lens epithelial cells in vitro . J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 360–363 (2008). https://doi.org/10.1007/s11596-008-0331-2
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DOI: https://doi.org/10.1007/s11596-008-0331-2