Summary
In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution (SM) and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration (n=6 in each group). The transferase in liver perfusion solution and intercellular adhesion molecule-1 (ICAM-1) and nitric oxide (NO) in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group (P>0.05), but there was significant difference as compared with HC-A group (P<0.05). At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.
Similar content being viewed by others
References
Nishimura Y, Takei Y, Kawano S et al. The F (ab’) 2 fragment of an anti-ICAM monoclonal antibody attenuates liver injury after orthotopic liver transplantation. Transplantation, 1996, 61:99–104
Takei Y, Marzi I, Kauffman F C et al. Leukocyte adhesion and cell death following orthotopic liver transplantation in the rat. Transplantation, 1991,59:959
Zeini M, hortelano S, Traves P G et al. Assessment of a dual regulatory role for NO in liver regeneration after partial hepatectomy: protection against apoptosis and retardation of hepatocyte proliferation. FASEB J, 2005,19(8):995–997
Oikawa K, Ohkohchi N, Sato M et al. Kupffer cells play an important role in the cytokine production and activation of nuclear factors of liver grafts from non-heart-beating donors. Transplant Int, 2002,15(8):397–405
Author information
Authors and Affiliations
Corresponding author
Additional information
Shiming WANG, male, born in 1965, M.D., Ph.D.
Rights and permissions
About this article
Cite this article
Wang, S., Dai, P., Xu, J. et al. Comparative study on the effects of self-made liver preservation solution on secretion of ICAM-1 and NO in rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 77–79 (2008). https://doi.org/10.1007/s11596-008-0119-4
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11596-008-0119-4