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Assessment of the effect of cardiomyocyte transplantation on left ventricular remodeling and function in post-infarction Wister rats by using high-frequency ultrasound

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Summary

The effects of cardiomyocyte grafting on left ventricular (LV) remodeling and function in rats with chronic myocardial infarction were evaluated using high-frequency ultrasound. Chronic myocardial infarction was induced in 50 Wister rats by ligating the left anterior descending artery. They were randomized into two groups: a trial group that received neonatal rat cardiomyocyte transplantation (n=25) and a control group which were given intramyocardial injection of culture medium (n=25). The left ventricular (LV) geometry and function were evaluated by high-frequency ultrasound before and 4 weeks after the cell transplantation. After the final evaluation, all rats were sacrificed for histological study. The results showed that 4 weeks after the cell transplantation, as compared with the control group, the LV end-systolic dimension, end-diastolic dimension, end-systolic volume and end-diastolic volume were significantly decreased and the LV anterior wall end-diastolic thickness, LV ejection fraction and fractional shortening were significantly increased in the trial group (P<0.01). Histological study showed that transplanted neonatal rat cardiomyocytes were found in all host hearts and identified by Brdu staining. It was suggested that transplantation of neonatal rat cardiomyocytes can reverse cardiac remodeling and improve heart function in chronic myocardial infarction rats. High-frequency ultrasound can be used as a reliable technique for the non-invasive evaluation of the effect of cardiomyocyte transplantation.

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References

  1. Zhu Z Y, Yan Z Y. Study on therapy of myocardial infarction by cardiomyocyte transplantation. Chin J Thorac Cardiovasc Surg, 2002,18:62–63

    Google Scholar 

  2. Chen J C, Jiang W P, Wu B M. Cell transplantation improves left ventricular function after myocardial infarction. Jiangsu Med, J 2002,10:767–769

    Google Scholar 

  3. Reinecke H, Zhang M, Bartosek T et al. Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts. Circulation, 1999,100:193–202

    CAS  PubMed  Google Scholar 

  4. Roell W, Lu Z J, Bloch W et al. Cellular cardiomyoplasty improves survival after myocardial injury. Circulation, 2002,105:2435–2441

    Article  PubMed  Google Scholar 

  5. Li R K, Jia Z Q, Weisel R D et al. Cardiomyocyte transplantation improves heart function. Ann Thorac Surg, 1996,62:654–661

    Article  CAS  PubMed  Google Scholar 

  6. Li R K, Mickle D A G, Weisel RD et al. In vivo survival and function of transplanted rat cardiomyocytes. Circ Res, 1996,78:283–288

    CAS  PubMed  Google Scholar 

  7. Li R K, Mickle D A G, Weisel R D et al. Natural history of fetal rat cardiomyocytes transplanted into adult rat myocardial scar tissue. Circulation, 1997,96(Suppl II):II 179–II 187

    Google Scholar 

  8. Sakakibara Y, Tambara K, Lu F et al. Cardiomyocyte transplantation does not reverse cardiac remodeling in rats with chronic myocardial infarction. Ann Thorac Surg, 2002,74:25–30

    Article  PubMed  Google Scholar 

  9. Litwin S E, Katz S E, Morgan J P et al. Serial echocardiographic assessment of left ventricular geometry and function after large myocardial infarction in the rat. Circulation, 1994,89:345–354

    CAS  PubMed  Google Scholar 

  10. Reinlib L, Field L. Cell transplantation as future therapy for cardiovascular disease: A workshop of the National Heart, Lung, and Blood Institute. Circulation, 2000,101:182–187

    Google Scholar 

  11. Scorsin M, Hagage A, Vilquin J T et al. Comparison of the effects of fetal cardiomyocyte and skeletal myoblast transplantation on postinfarction left ventricular function. J Thorac Cardiovasc Surg, 2000,119:1169–1175

    Article  CAS  PubMed  Google Scholar 

  12. Muller-Ehmsen J, Peterson K L, Kedes L et al. Rebuilding a damaged heart: long-term survival of transplanted neonatal rat cardiomyocytes after myocardial infarction and effect on cardiac function. Circulation, 2002, 105:1720–1726

    Article  PubMed  Google Scholar 

  13. Huang G Q, Liu H, Shu X H et al. Echocardiography evaluation of effect of autologous bone marrow stem cells mobilization with granulocyte-colony stimulating factor on left ventricular remodeling and function in post-infarction rats. Chin J Ultrasonogr, 2004,13:848–852

    Google Scholar 

  14. Schwarz E R, Pollick C, Dow J et al. A small animal model of non-ischemic cardiomyopathy and its evaluation by transthoracic echocardiography. Cardiovasc Res, 1998,39:216–223

    Article  CAS  PubMed  Google Scholar 

  15. Kanno S, Lerner D L, Schuessler R B et al. Echocardiographic evaluation of ventricular remodeling in a mouse model of myocardial infarction. J Am Soc Echocardiogr, 2002,15:601–609

    Article  PubMed  Google Scholar 

  16. Scherrer-Crosbie M, Steudel W, Hunziker P R et al. Three-dimensional echocardiographic assessment of left ventricular wall motion abnormalities in mouse myocardial infarction. J Am Soc Echocardiogr, 1999,12:834–840

    Article  CAS  PubMed  Google Scholar 

  17. Zhang G X, Shen X D, PU S Y et al. Serial high frequency ultrasound assessment of the progressive change of the ventricular remodeling and function after myocardial infarction in rat. Chin J Ultrasound Med, 1997, 13:7–10

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Ultrasound, Union Hospital, Tongji Medical college, Huazhong University of Science and Technology, Wuhan, 430022, China

    Zhang Jing  (张 静), Xie Mingxing  (谢明星), Wang Xinfang  (王新房), Lü Qing  (吕 清), Lang Mingjian  (朗明建) & Deng Binhua  (邓斌华)

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  1. Zhang Jing  (张 静)
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  2. Xie Mingxing  (谢明星)
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  3. Wang Xinfang  (王新房)
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  4. Lü Qing  (吕 清)
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  5. Lang Mingjian  (朗明建)
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  6. Deng Binhua  (邓斌华)
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Zhang, J., Xie, M., Wang, X. et al. Assessment of the effect of cardiomyocyte transplantation on left ventricular remodeling and function in post-infarction Wister rats by using high-frequency ultrasound. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 696–699 (2007). https://doi.org/10.1007/s11596-007-0620-1

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  • DOI: https://doi.org/10.1007/s11596-007-0620-1

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