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Relationship between the expression of telomerase and human papillomavirus infection in invasive uterine cervical carcinoma

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Summary

Telomerase activity was examined in invasive cervical carcinoma to assess whether it is activated during cervical malignant transformation and to look for its possible association with human papillomavirus (HPV) infection. Histologically confirmed invasive cervical carcinomas and benign cervices were assayed for telomerase activity by using a modified telomere repeat amplification protocol (TRAP). The same cases were subjected to polymerase chain reaction (PCR) detection of HPV by using consensus primers and type-specific (HPV types 16 and 18) primers. Telomerase activity was detected in 40 of 45 (88.9%) invasive cervical carcinomas and 2 (all chronic cervicitis) of 50 (4%) benign cervical lesions. HPV was detected in 36 (24 HPV-16 and 4 HPV-18 cases) of 45 (80%) invasive cervical carcinomas and 20 (11 HPV-16 and 1 HPV-18 cases) of 50 (40%) benign cervical changes. There was a significant correlation between the expression of telomerase with histological grade (ω=0.44, P<0.005), but no correlation was found between telomerase expression and HPV-18 (P>0.05). Although larger sample studies are needed, there seems to be a clear association between telomerase upregulation and HPV status, mainly HPV-16 infection.

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References

  1. McMurray H R, Nguyen D, Westbrook T F et al. Biology of human papillomaviruses. Int J Exp Pathol, 2001, 82(1): 15–33

    Article  PubMed  CAS  Google Scholar 

  2. Munoz N, Bosch F X, de Sanjose S et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med, 2003,348(6):518–527

    Article  PubMed  Google Scholar 

  3. zur Hausen H. Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer, 2002,2(5):342–350

    Article  PubMed  Google Scholar 

  4. Bisoffi M, Heaphy C M, Griffith J K. Telomeres: prognostic markers for solid tumors. Int J Cancer, 2006,119(10):2255–2260

    Article  PubMed  CAS  Google Scholar 

  5. Liu J P. Studies of the molecular mechanisms in the regulation of telomerase activity. FASEB J, 1999; 13(15):2091

    PubMed  CAS  Google Scholar 

  6. Bosserhoff A K, Glabl A, Stolz W et al. Detection of telomerase activity in skin, melanocytic nevi and melanoma by telomerase PCR ELISA. Biochemica, 1997,3:16–18

    Google Scholar 

  7. Zheng P S, Iwasaka T, Yokoyama M et al. Telomerase activation in in vitro and in vivo cervical carcinogenesis. Gynecol Oncol, 1997, 66(2): 222–226

    Article  PubMed  CAS  Google Scholar 

  8. Hiyama E, Gollahon L, Kataoka T et al. Telomerase activity in human breast tumors. J Natl Cancer Inst, 1996, 88(2):116–122

    Article  PubMed  CAS  Google Scholar 

  9. Meisels A, Fortin R. Condylomatous lesions of the cervix and vagina. I. Cytologic patterns. Acta Cytol, 1976, 20(6):505–509

    PubMed  CAS  Google Scholar 

  10. Munoz N, Bosch F X. Current views on the epidemiology of HPV and cervical cancer. In: Lacey C, ed. Papillomavirus reviews: current research on papillomaviruses. Leeds: Leeds University Press, 1996.227–237

    Google Scholar 

  11. Tsezou A, Oikonomou P, Kollia P et al. The Role of Human Telomerase Catalytic Subunit mRNA Expression in Cervical Dysplasias. Exp Biol Med, 2005, 230(4):263–270

    CAS  Google Scholar 

  12. Nair P, Jayaprakash P G, Nair M K et al. Telomerase, p53 and human papillomavirus infection in the uterine cervix. Acta Oncol, 2000, 39(1):65–70

    Article  PubMed  CAS  Google Scholar 

  13. Hiroyuki F, and Minoru I. Human Papilloma Virus (HPV) and cervical cancer. J Med Invest, 2002, 49(3–4):24–133

    Google Scholar 

  14. Wisman G B, Hollema H, de Jong S et al. Telomerase activity as a biomarker for (pre) neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear. J Clin Oncol, 1998,16(6):2238

    PubMed  CAS  Google Scholar 

  15. Yashima K, Ashfaq R, Nowak J et al. Telomerase activity and expression of its RNA component in cervical lesions. Cancer, 1998, 82(7):1319–1327

    Article  PubMed  CAS  Google Scholar 

  16. Sudip S, Vijay G R, Randeep G et al. Telomerase—a potential molecular marker of lung and cervical cancer. Clin Chem Lab Med, 2002, 40(10): 994–1001

    Article  Google Scholar 

  17. Satoru M, Cecilia A L, Salvador L V et al. The Role of Human Papilloma Virus in the Molecular Biology of Cervical Carcinogenesis. Kobe J Med Sci, 2004, 50(1):9–19

    Google Scholar 

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Authors and Affiliations

  1. Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Sima Ni  (司马妮), Wang Wei  (王薇) & Wang Shixuan  (王世宣)

  2. Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Sima Ni  (司马妮)

  3. Department of Gynecology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Cai Liping  (蔡丽萍) & Zhu Yuanfang  (朱元方)

Authors
  1. Sima Ni  (司马妮)
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  2. Cai Liping  (蔡丽萍)
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  3. Zhu Yuanfang  (朱元方)
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  4. Wang Wei  (王薇)
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  5. Wang Shixuan  (王世宣)
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  6. Ma Ding  (马丁)
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Corresponding author

Correspondence to Sima Ni  (司马妮).

Additional information

SIMA Ni, female, born in 1979, Doctor in Charge

This project was supported by grants from the National Natural Science Foundation of China (No. 30672227, No. 30170976) and the Program 973 of China (No. 2002CB513100).

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Sima, N., Cai, L., Zhu, Y. et al. Relationship between the expression of telomerase and human papillomavirus infection in invasive uterine cervical carcinoma. J. Huazhong Univ. Sc. Technol. 27, 451–453 (2007). https://doi.org/10.1007/s11596-007-0426-1

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  • DOI: https://doi.org/10.1007/s11596-007-0426-1

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