Summary
The effects of salvia miltiorrhiza Bge (SMB) on left ventricular hypertrophy (LVH) and the expression of tumor necrosis factor-α (TNF-α) in the left ventricle of spontaneously hypertensive rats and the action mechanism were investigated. Normal Wistar-kyoto (WKY) rats were used as negative control, and spontaneously hypertensive rats (SHR) were randomly assigned to receive placebo or SMB. SMB (1 g/kg·d) was injected intraperitoneally for 12 weeks. Systolic blood pressure (SBP) and left ventricular mass index (LVMI) were measured. HE, VG and immunohistochemical staining combined with computed morphometry were employed to evaluate the cardiomyocyte size, diameter, the collagen volume fraction (CVF), perivascular circumferential area (PVCA), and tumor necrosis factor-α (TNF-α) expression in the left ventricular tissue. The results showed, as compared with WKY rats, the SBP, LVMI, cardiomyocyte size, diameter, CVF, PCVA, and TNF-α expression were increased markedly in the 20-week-old spontaneously hypertensive rats. SMB decreased LVMI (P<0.01), size of cardiomyocytes (P<0.01), collagen volume fraction (P<0.01), perivascular circumferential area (P<0.01), and TNF-α expression (P<0.01), but had no effect on SBP (P>0.05). It was suggested that chronic administration of SMB could inhibit and reverse the development of LVH in spontaneously hypertensive rats independent of BP. TNF-α may be involved in the reversal mechanism of LVH by SMB.
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References
Sadoshima J, Montagne O, Wang Q et al. The MEKK1 JNK pathway plays a protectiverole in pressure overload but does not mediate cardiac hypertrophy. J Clin Invest, 2002,110(2):271–279
Nagueh S F, Stetson S J, Lakkis N M. Decreased expression of tumor necrosis factor-α and regression of hypertrophy after nonsurgical septal reduction therapy for patients with hypertrophic obstructive cardiomyopathy. Circulation, 2001,103:1844–1850
Bergman M R, Kao R H, McCune S A et al. Myocardial tumor necrosis factor-α secretion in hypertensive and heart failure-prone rats. Am J Physiol, 1999,277(2):H543–H550
Stamm C, Friehs I, Douglas B. Inhibition of tumor necrosis factor-α improves post-ischemic recovery of hypertrophied hearts. Circulation, 2001 (12 suppl 1),104:1350–1355
Takahashi K, Ouyang X, Komatsu K et al. Sodium tanshinone IIA sulfonate derived from Danshen (Salvia miltiorrhiza) attenuates hypertrophy induced by angiotensin II in cultured neonatal rat cardial cells. Biochem Pharmacol, 2002,64(4):745–749
Ouyang X, Takahashi K, Komatsu K et al. Protective effect of Salvia miltiorrhiza on angiotensin II-induced hypertrophic responses in neonatal rat cardiol cells. Jpn J Pharmacol, 2001,87(4):289–296
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SUN Lianping, male, born in 1966, Associate Professor
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Sun, L., Zheng, Z. Effect of salvia miltiorrhiza Bge on left ventricular hypertrophy and the expression of tumor necrosis factor-α in spontaneously hypertensive rats. J. Huazhong Univ. Sc. Technol. 27, 245–247 (2007). https://doi.org/10.1007/s11596-007-0307-7
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DOI: https://doi.org/10.1007/s11596-007-0307-7