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Anti-cancer effects of deguelin on human leukemia K562 and K562/ADM cells In Vitro

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Summary

In order to investigate the anti-cancer effects of deguelin and on K562 and K562/ADM cells in vitro and the underlying molecular mechanism and compare the cytotoxicity of deguelin on K562, K562/ADM cells and human peripheral blood mononuclear cells (PBMCs). The effects of deguelin on cell proliferation were assessed by MTT assay. Apoptosis were detected by Annexin V/PI double-labeled cytometry. The effects of deguelin on the cell cycle were studied by a propidium iodide method. Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells. The ratio of IC50 value of deguelin of 24 h on K562/ADM cells to K562 cells was only 1.27, which was significantly lower than the ratio of IC50 value of ADM (higher than 20). Deguelin could induce apoptosis of K562 cells and K562/ADM cells. K562 cells were arrested at G2/M phase while K562/ADM cells were arrested at G0/G1 phase. Our results suggested that deguelin was a novel anti-leukemia agents with high efficacy and low toxicity and it is also a promising agent for reversing drug resistance.

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Authors and Affiliations

  1. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Wu Qiuling  (吴秋玲), Chen Yan  (陈燕), Liu Hongli  (刘红利) & He Jing  (何静)

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  1. Wu Qiuling  (吴秋玲)
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  2. Chen Yan  (陈燕)
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  3. Liu Hongli  (刘红利)
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  4. He Jing  (何静)
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Corresponding author

Correspondence to Chen Yan  (陈燕).

Additional information

WU Qiuling, female, born in 1975, Attending Physician

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30472267).

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Wu, Q., Chen, Y., Liu, H. et al. Anti-cancer effects of deguelin on human leukemia K562 and K562/ADM cells In Vitro . J. Huazhong Univ. Sc. Technol. 27, 149–152 (2007). https://doi.org/10.1007/s11596-007-0210-2

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  • DOI: https://doi.org/10.1007/s11596-007-0210-2

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