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Effect of interleukin-1β on IA and IK currents in cultured murine trigeminal ganglion neurons

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Summary

To investigate the effect of interleukin-1β (IL-1β) on IA and IK currents in cultured murine trigeminal ganglion (TG) neurons, whole-cell patch clamp technique was used to record the IA and IK currents before and after 20 ng/mL IL-1β perfusion. Our results showed that 20 ng/mL IL-1β inhibited IA currents (18.3 ± 10.7)% (n=6, P<0.05). IL-1β at 20 ng/mL had no effect on G-V curve of IA but moved the H-infinity curve V0.5 from −36.6±6.1 mV to −42.4±5.2 mV (n=5, P<0.01). However, 20 ng/mL IL-1β had effect on neither the amplitude nor the G-V curve of IK. IL-1β was found to selectively inhibit IA current in TG neurons and the effect may contribute to hyperalgesia under various inflammatory conditions.

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Authors and Affiliations

  1. Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Pan Jianping  (潘建萍), Liu Lieju  (刘烈炬), Yang Fei  (杨斐), Cao Xuehong  (曹雪红), Fu Hui  (付晖) & Ming Zhangxin  (明章银)

  2. Departments of Anesthesiology, Duke University Medical Center, Durham, NC, 27710, USA

    Liu Lieju  (刘烈炬)

Authors
  1. Pan Jianping  (潘建萍)
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  2. Liu Lieju  (刘烈炬)
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  3. Yang Fei  (杨斐)
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  4. Cao Xuehong  (曹雪红)
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  5. Fu Hui  (付晖)
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  6. Ming Zhangxin  (明章银)
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Additional information

PAN Jianping, female, born in 1979, Master in Pharmacology

The project was supported by a grant from the National Natural Sciences Foundation of China (No. 30271500).

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Pan, J., Liu, L., Yang, F. et al. Effect of interleukin-1β on IA and IK currents in cultured murine trigeminal ganglion neurons. J. Huazhong Univ. Sc. Technol. 27, 131–134 (2007). https://doi.org/10.1007/s11596-007-0205-z

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  • DOI: https://doi.org/10.1007/s11596-007-0205-z

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