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Role of connective tissue growth factor in extracellular matrix degradation in renal tubular epithelial cells

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Summary

In order to investigate the effects of connective tissue growth factor (CTGF) antisense oligodeoxynucleotide (ODN) on plasminogen activator inhibitor-1 (PAI-1) expression in renal tubular cells induced by transforming growth factor β1 (TGF-β1) and to explore the role of CTGF in the degradation of renal extracellular matrix (ECM), a human proximal tubular epithelial cell line (HKC) was cultured in vitro. Cationic lipid-mediated CTGF antisense ODN was transfected into HKC. After HKC were stimulated with TGF-β1 (5 μg/L), the mRNA level of PAI-1 was detected by RT-PCR. Intracellular PAI-1 protein synthesis was assessed by flow cytometry. The secreted PAI-1 in the media was determined by Western blot. The results showed that TGF-β1 could induce tubular CTGF and PAI-1 mRNA expression. The PAI-1 mRNA expression induced by TGF-β1 was significantly inhibited by CTGF antisense ODN. CTGF antisense ODN also inhibited intracellular PAI-1 protein synthesis and lowered the levels of PAI-1 protein secreted into the media. It was concluded that CTGF might play a crucial role in the degradation of excessive ECM during tubulointerstitial fibrosis, and blocking the biological effect of CTGF may be a novel way in preventing renal fibrosis.

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Authors and Affiliations

  1. Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Zhang Chun  (张春), Zhu Zhonghua  (朱忠华), Liu Jianshe  (刘建社), Yang Xiao  (杨晓), Fu Ling  (付玲) & Deng Anguo  (邓安国)

Authors
  1. Zhang Chun  (张春)
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  2. Zhu Zhonghua  (朱忠华)
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  3. Liu Jianshe  (刘建社)
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  4. Yang Xiao  (杨晓)
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  5. Fu Ling  (付玲)
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  6. Deng Anguo  (邓安国)
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Corresponding author

Correspondence to Zhu Zhonghua  (朱忠华).

Additional information

ZHANG Chun, male, born in 1977, Associate Professor, M.D., Ph.D.

This project was supported by a grant from the Science & Technology Foundation of Hubei Province (No. 2003 AA 301C14).

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Zhang, C., Zhu, Z., Liu, J. et al. Role of connective tissue growth factor in extracellular matrix degradation in renal tubular epithelial cells. J. Huazhong Univ. Sc. Technol. 27, 44–47 (2007). https://doi.org/10.1007/s11596-007-0113-2

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  • DOI: https://doi.org/10.1007/s11596-007-0113-2

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