Summary
To investigate the expression of plasmacytoid dendritic cells (pDCs), interferon regulatory factor-7 (IRF-7) and interferon alpha (IFN-α) mRNA in skin lesions of patients with psoriasis vulgaris, the expressions of plasmacytoid dendritic cells, IRF-7, IFN-α mRNA in the lesional skin of psoriasis vulgaris were detected by immunohistochemical technique (SP) and RT-PCR. Normal skin of healthy volunteers, serving as control, was also tested. The immunohistochemical study showed that the expression of pDCs in the psoriatic lesions was significantly higher than that in the normal controls. RT-PCR showed that the mRNA expression of IRF-7 was much higher than that in normal controls, but no difference in the expression of IFN-α mRNA was found between two groups. Our findings indicate that up-regulated expression of pDCs, IRF-7mRNA might be involved in the pathogenesis of psoriasis.
Similar content being viewed by others
References
Olweus J, BitMansour A, Warnke R et al. Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc Natl Acad Sci USA, 1997,94:12551
Liu Y J. IPC: professional type I interferon-producing cells and plasmacytoid dendritic cell precursors. Annu Rev Immunol, 2005,23:275–306
Kawai T, Sato S, Ishii K J et al. Interferonalpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol, 2004,5(10):1061–8
Krug A, Towarowski A, Britsch S et al. Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur J Immunol, 2001,31:3026
Farkas L, Beiske K, Lund-Johansen F et al. Plasmacytoid dendritic cells (natural interferon-alpha/beta-producing cells) accumulate in cutaneous lupus erythematosus lesions. Am J Pathol, 2001,159:237–243
Van Krinks C H, Matyszak M K, Gaston J S. Characterization of plasmacytoid dendritic cells in inflammatory arthritis synovial fluid. Rheumatology (Oxford), 2004,43:453–460
Gottenberg J E, Cagnard N, Lucchesi C et al. Activation of IFN pathways and plasmacytoid dendritic cell recruitment in target organs of primary Sjogren’s syndrome. Proc Natl Acad Sci USA, 2006,103(8):2770–5
Boyman O, Hefti H P, Conrad C et al. Spontaneous development of psoriasis in a new animal model shows an essential role for resident T cells and tumor necrosis factor-alpha. J Exp Med, 2004,199:731–736
Theofilopoulos A N, Baccala R, Beutler B et al. Type I interferons in immunity and autoimmunity. Annu Rev Immunol, 2005, 23:307–335
Ladoyanni E, Nambi R. psoriasis exacerbated by interferon-alpha in a patient with chronic myeloid leukemia. J Drugs Dermatol, 2005,4(2): 221–2
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, J., Tan, Z., Liu, H. et al. Expression of plasmacytoid dendritic cells, IRF-7, IFN-α mRNA in the lesions of psoriasis vulgaris. J. Huazhong Univ. Sc. Technol. 26, 747–749 (2006). https://doi.org/10.1007/s11596-006-0634-0
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11596-006-0634-0