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Intrastriatal gene transfer of vascular endothelial growth factor rescues dopaminergic neurons in a rat Parkinson’s disease model

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Summary

To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson’s disease (PD), we constructed recombinant replication-deficent adenoviral vectors carrying the gene of VEGF165 (Ad-VEGF), and injected Ad-VEGF (or Ad-LacZ and PBS as controls) into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase (TH) immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals. It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.

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References

  1. Storkebaum E, Lambrechts D, Carmeliet P. VEGF: once regarded as a specific angiogenic factor, now implicated in neuroprotection. Bioessays, 2004,26:943–954

    Article  PubMed  CAS  Google Scholar 

  2. Storkebaum E, Carmeliet P. VEGF: a critical player in neurodegeneration. J Clin Invest, 2004,113:14–18

    Article  PubMed  CAS  Google Scholar 

  3. Wang C Q, Wang J N, Huang Y Z et al. Construction and characterization of replication-deficient adenvoiral vector encoding for human vascular endothelial growth factor 165. Chin J of Clin Lab Sci (Chin), 2002,20:156–158

    Google Scholar 

  4. Przedborski S, Levivier M, Jiang H et al. Dose-dependent lesions of the dopaminergic nigrostriatal pathway induced by intrastriatal injection of 6-hydroxydopamine. Neuroscience, 1995,67:631–647

    Article  PubMed  CAS  Google Scholar 

  5. Hudson J L, van Horne C G, Stromberg I et al. Correlation of apomorphine-and amphetamine-induced turning with nigrostriatal dopamine content in unilateral 6-hydroxy-dopamine lesioned rats. Brain Res, 1993,626: 167–174

    Article  PubMed  CAS  Google Scholar 

  6. Matsuzaki H, Tamatani M, Yamaguchi A et al. Vascular endothelial growth factor rescues hippocampal neurons from glutamate-induced toxicity: signal transduction cascades. FASEB J, 2001,15:1218–1220

    PubMed  CAS  Google Scholar 

  7. Svensson B, Peters M, Konig H G et al. Vascular endothelial growth factor protects cultured rat hippocampal neurons against hypoxic injury via an antiexcitotoxic, caspase-independent mechanism. J Cereb Blood Flow Metab, 2002,22:1170–1175

    Article  PubMed  CAS  Google Scholar 

  8. Yasuhara T, Shingo T, Kobayashi K et al. Neuroprotective effects of vascular endothelial growth factor (VEGF) upon dopaminergic neurons in a rat model of Parkinson’s disease. Eur J Neurosci. 2004,19:1494–1504

    Article  PubMed  Google Scholar 

  9. Jeffrey H K, Marina E E, Jocelyne B et al. Neurodegeneration Prevented by Lentiviral Vector Delivery of GDNF in Primate Models of Parkinson’s Disease. Science, 2000,290:767–773

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

    Tian Youyong M. D., Ph.D.  (田有勇) (male), Sun Shenggang  (孙圣刚), Chen Xiaowu  (陈小武) & Qiao Xian  (乔娴)

  2. Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Tang Cuiju  (汤翠菊)

  3. Institute of Clinical Medicine, the Affiliated People’s Hospital of Yunyang Medical College, Shiyan, 442000, China

    Wang Jianing  (王家宁)

Authors
  1. Tian Youyong M. D., Ph.D.  (田有勇)
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  2. Sun Shenggang  (孙圣刚)
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  3. Tang Cuiju  (汤翠菊)
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  4. Wang Jianing  (王家宁)
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  5. Chen Xiaowu  (陈小武)
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  6. Qiao Xian  (乔娴)
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Correspondence to Sun Shenggang  (孙圣刚).

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Tian, Y., Sun, S., Tang, C. et al. Intrastriatal gene transfer of vascular endothelial growth factor rescues dopaminergic neurons in a rat Parkinson’s disease model. J. Huazhong Univ. Sc. Technol. 26, 670–673 (2006). https://doi.org/10.1007/s11596-006-0611-7

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  • DOI: https://doi.org/10.1007/s11596-006-0611-7

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