Summary
The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent protein (GFP) driven by CEA promoter and human cytomegalovirus (CMV) promoter were relatively constructed and named plasmid pCEA-EGFP and pCMV-GFP respectively. The intensity of fluorescence was detected by fluorescence microscope and flow cytometry analysis after the pCEA-GFP and pSNAV-GFP plasmids were transfected into A549 cells through liposome respectively. The results showed (4.08±0.63) % of the A549 cells transfected with pCEA-AFP plasmid expressed, significantly lower than that of the A549 cells transfected with pCMV-GFP [(43.27±3.54) %]. It was suggested that ectogenic human CEA promoter in lung adenocarcinoma cells A549 was weakly expressed. The distinct specificity of CEA promoter in CEA high expression cells was regarded as a tool in selective gene therapy, but the transcription activity of ectogenic human CEA promoter was needed to increase in the future.
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This project was supported by a grant from Hubei Provincial Natural Sciences of China (No. 2003ABA144)
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Xiong, W., Fang, H., Xu, Y. et al. Transcription activity of ectogenic human carcinoembryonic antigen promoter in lung adenocarcinoma cells A549. J. Huazhong Univ. Sc. Technol. 26, 517–519 (2006). https://doi.org/10.1007/s11596-006-0507-6
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DOI: https://doi.org/10.1007/s11596-006-0507-6