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In Vitro Angiogenic Behavior of HUVECs on Biomimetic SF/SA Composite Scaffolds

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Abstract

The 60Fc and 70Fc SF/SA blend scaffolds were prepared to mimic the functions of the native ECM for skin regeneration. Human Umbilical Vein Endothelial Cells (HUVECs) were used to examine the cell cytotoxicity, adhesion, growth factors secretion and the gene expression of associated angiogenic factors. Cell proliferation, adhesion and live-dead analyses showed that HUVECs could better attach, grow, and proliferate on the 70Fc scaffolds compared with 60Fc scaffolds and unmodified controls. Furthermore, the 70Fc scaffolds showed higher levels of specific angiogenic proteins and genes expression as well. This study suggests that the involvement of higher composition of SF (about 70%) than that of SA on the blended scaffolds could be advantageous as it is more suitable to promote angiogenesis, which is potential for vascularization during skin repair.

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Authors and Affiliations

  1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China

    Omar Ramadhan Kombo, Xinyu Wang  (王欣宇), Ying Shen, Jiawei Liu, Xianzhen Dong, Qi Shao, Yanpiao Long, Kuo Dong, Shahd Fateh El-Rahman Elkhider Bakhet & Binbin Li  (李宾斌)

  2. Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan, 430070, China

    Omar Ramadhan Kombo, Xinyu Wang  (王欣宇), Ying Shen, Jiawei Liu, Xianzhen Dong, Qi Shao, Yanpiao Long, Kuo Dong, Shahd Fateh El-Rahman Elkhider Bakhet & Binbin Li  (李宾斌)

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  1. Omar Ramadhan Kombo
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  2. Xinyu Wang  (王欣宇)
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  9. Shahd Fateh El-Rahman Elkhider Bakhet
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  10. Binbin Li  (李宾斌)
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Correspondence to Xinyu Wang  (王欣宇) or Binbin Li  (李宾斌).

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Supported by the Major Special Projects of Technological Innovation of Hubei Province, China (No. 2017ACA168) and the National Key R&D Program of China (No.2017YFC1103800)

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Kombo, O.R., Wang, X., Shen, Y. et al. In Vitro Angiogenic Behavior of HUVECs on Biomimetic SF/SA Composite Scaffolds. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 36, 456–464 (2021). https://doi.org/10.1007/s11595-021-2430-x

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