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Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins

  • Biomaterials
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Abstract

A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe3O4@TiO2 (Fe3O4@mTiO2) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe3O4@mTiO2-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe3O4@mTiO2-AIPA was successfully utilized to separate α-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and α-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe3O4@mTiO2-AIPA for application in phosphoprotein enrichment.

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Authors and Affiliations

  1. School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070, China

    Qiuhan Tang  (汤秋菡)

  2. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China

    Rui Zhao, Qi Lu  (陆琦) & Guangyan Qing  (卿光焱)

  3. Research & Development Center, Jushi Group Co., Ltd, Tongxiang, 314500, China

    Qi Lu  (陆琦)

Authors
  1. Qiuhan Tang  (汤秋菡)
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  2. Rui Zhao
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  3. Qi Lu  (陆琦)
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  4. Guangyan Qing  (卿光焱)
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Corresponding authors

Correspondence to Qi Lu  (陆琦) or Guangyan Qing  (卿光焱).

Additional information

Funded by the National Natural Science Foundation of China (Nos. 51473131, 21275114, 51533007 and 51521001), the Major State Basic Research Development Program of China (973 Program) (No. 2013CB933002), Hubei Provincial Department of Education for Financial Assistance Through the “Chutian Scholar” Program, and Hubei Provincial Natural Science Foundation of China (No. 2014CFA039)

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Tang, Q., Zhao, R., Lu, Q. et al. Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 34, 752–759 (2019). https://doi.org/10.1007/s11595-019-2113-z

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  • DOI: https://doi.org/10.1007/s11595-019-2113-z

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