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Synthesis and evaluation of novel chitosan derivatives for gene delivery

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Abstract

A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293T and HeLa cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors

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Authors and Affiliations

  1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China

    Fang Fu  (付芳), Xiong Liu, Bo Lü  (吕波), Hua Zheng, Zhijun Huang, Peihu Xu, Haixing Xu, Yihua Yin, Xia Liu & Xueqiong Zhang

  2. Luo Tian Wan Mizhai Hospital, Huanggang, 438600, China

    Guoyi Qu

Authors
  1. Fang Fu  (付芳)
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  2. Xiong Liu
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  3. Bo Lü  (吕波)
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  4. Guoyi Qu
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  5. Hua Zheng
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  6. Zhijun Huang
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  7. Peihu Xu
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  8. Haixing Xu
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  9. Yihua Yin
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  10. Xia Liu
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  11. Xueqiong Zhang
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Corresponding author

Correspondence to Bo Lü  (吕波).

Additional information

Funded by the National Natural Science Foundation of China (Nos.21204071, 51273156), the Natural Science Foundation of Hubei Province (2014CFB833), and the Innovation Research Fund of Wuhan university of Technology (20121049720006)

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Fu, F., Liu, X., Lü, B. et al. Synthesis and evaluation of novel chitosan derivatives for gene delivery. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 30, 852–858 (2015). https://doi.org/10.1007/s11595-015-1241-3

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  • DOI: https://doi.org/10.1007/s11595-015-1241-3

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