Abstract
The effects of five chito-oligomers, from dimer to hexamer (chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose) separated from chitosan oligosaccharides, on nuclear factor -kappaB (NF-κB) signaling pathway were investigated by using luciferase assay and laser scanning microscopy. The expression of NF-κB downstream genes (cyclin D1, TNFα and IL-6) were tested by real time PCR. We found that all five chitosan oligosaccharides increased NF-κB-dependent luciferase gene expression and NF-κB downstream genes transcription, and the most significant were chitotetraose and chitohexaose. In addition, laser scanning microscopy experiments showed that chitotetraose and chitohexaose also activated the p65 subunite of NF-κB translocating from cytoplasm to nucleus, which suggested that they were the most potent activators of NF-κB signaling pathway.
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Funded by the State High-Technology R&D Project of China (863 Program) (2007AA091603)
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Li, X., Zhou, C., Chen, X. et al. Effects of five chitosan oligosaccharides on nuclear factor-kappa B signaling pathway. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 27, 276–279 (2012). https://doi.org/10.1007/s11595-012-0452-0
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DOI: https://doi.org/10.1007/s11595-012-0452-0