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In vitro blood compatibility of polyethylene terephthalate with covalently bounded hirudin on surface

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Abstract

Polyethylene terephthalate (PET, Dacron) was modified by surface immobilization of hirudin with glutaraldehyde(GA) as coupling reagent to improve the blood compatibility. Hirudin-immobilized PETs were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The blood compatibility of the PETs was evaluated by platelet adhesion evaluation and fibrinogen conformational change measurements in vitro. The results showed the decrease of platelet adhesion and activation on hirudin-immobilized PET with increasing of glutaraldehyde concentration. Fibrinogen experiment showed that fibrinogen adherence and conformational changes of PET-HRD were less than those of untreated PET, which made the materials difficult to form thrombus. The proper reason of blood compatibility improvement was low interface tension between hirudin-immobilized PETs and blood, as well as blood proteins, and low ratio of dispersive/polar component of the surface energy(γ sd/γ sp) and high hydrophilicity.

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Authors and Affiliations

  1. School of Petroleum Engineering, Southwest Petroleum University, Chengdu, 610500, China

    Fang Li  (李方)

  2. Lab of Surface Modification of Artificial Organs of Sichuan, Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu, 610031, China

    Fang Li  (李方), Jin Wang & Nan Huang

Authors
  1. Fang Li  (李方)
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  2. Jin Wang
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  3. Nan Huang
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Correspondence to Fang Li  (李方).

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Funded by the National Natural Science Foundation of China (No.50203011)

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Li, F., Wang, J. & Huang, N. In vitro blood compatibility of polyethylene terephthalate with covalently bounded hirudin on surface. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 26, 950–954 (2011). https://doi.org/10.1007/s11595-011-0343-9

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  • DOI: https://doi.org/10.1007/s11595-011-0343-9

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