Skip to main content
SpringerLink
Log in

In vitro biomineralization of glutaraldehyde crosslinked chitosan/glutamic acid films

  • Published:
Journal of Wuhan University of Technology-Mater. Sci. Ed. Aims and scope Submit manuscript

Abstract

In vitro biomineralization of glutaraldehyde crosslinked chitosan/glutamic acid films were studied. IR and ESCA (electron spectroscopy for chemical analysis) determinations confirm that chitosan and glutamic acid are successfully crosslinked by glutaraldehyde to form chitosan-glutamic acid surfaces. Composite films were soaked in saturated Ca(OH)2 solution for 8 d and then immersed in simulated body fluid (SBF) for more than 20 d. Morphological characterizations and structure of calcium phosphate coatings deposited on the films were studied by SEM, XRD, and EDAX (energy dispersive X-ray analysis). Initially, the treatment in SBF results in the formation of single-layer calcium phosphate particles over the film surface. As immersion time increases, further nucleation and growth produce the simulated calcium-carbonate hydroxyapatite coating. ICP results show Ca/P ratio of calcium phosphate coating is a function of SBF immersion time. The inducing of glutamic acid improves the biomineralization property of chitosan films.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M M Beppu, C G Aimoli. Influence of Acetylation on in Vitro Chitosan Membrane Biomineralization[J]. Key Eng. Mater., 2004, 254-2: 311–314

    Article  Google Scholar 

  2. A C A Wan, E Khor, G W Hastings. Preparation of a Chitin-apatite Composite by in Situ Precipitation onto Porous Chitin Scaffolds[J]. J. Biomed. Mater. Res., 1998, 41: 541–548

    Article  PubMed  CAS  Google Scholar 

  3. A C A Wan, E Khor, J M Wong, et al. Promotion of Calcification on Carboxymethylchitin Discs[J]. Biomaterials, 1996, 17: 1 529–1 534

    Article  CAS  Google Scholar 

  4. Y Yokogawa, J PazReyes, M R Mucalo, et al. Growth of Calcium Phosphate on Phosphorylated Chitin Fibers[J]. J. Mater. Sci. Mater. Med., 1997, 8: 407–412

    Article  PubMed  CAS  Google Scholar 

  5. H K Varma, Y Yokogawa, F F Espinosa, et al. Porous Calcium Phosphate Coating over Phosphorylated Chitosan Film by a Biomimetic Method[J]. Biomaterials, 1999, 20: 879–884

    Article  PubMed  CAS  Google Scholar 

  6. M M Beppu, C C Santana. In Vitro Biomineralization of Chitosan[J]. Key Eng. Mater., 2001, 192-1: 31–34

    Article  Google Scholar 

  7. T Kato, A Sugawara, N Hosoda. Calcium Carbonate-organic Hybride Materials[J]. Advanced Materials, 2002, 14(12): 869–877

    Article  CAS  Google Scholar 

  8. C Muzzarelli, R A A Muzzarelli. Natural and Artificial Chitosan-inorganic Composites[J]. J. Inorg. Biochem., 2002, 92: 89–94

    Article  PubMed  CAS  Google Scholar 

  9. G Falini, S Albeck, S Weiner, et al. Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules[J]. Science, 1996, 271: 67–69

    Article  ADS  Google Scholar 

  10. A C Oyrton, J Monteiro, A Claudio. Some Studies of Crosslinking Chitosan-glutaraldehyde Interaction in a Homogeneous System[J]. Int. J. Biol. Macromol., 1999, 26: 119–125

    Article  Google Scholar 

  11. Y Zhang, M Zhang. Microstructurural and Mechanical Characterization of Chitosan Scaffolds Reinforced by Calcium Phosphates[J]. J. Non-crystal. Solids, 2001, 282: 159–164

    Article  ADS  CAS  Google Scholar 

  12. T W Chung, Y F Lu, S S Wang, et al. Growth of Human Endothelial Cells on Photochemically Grafted Gly-arg-gly-asp(GRGD) Chitosans[J]. Biomaterials, 2002, 23: 4 803–4 809

    CAS  Google Scholar 

  13. P Calvert. Biomimetic Mineralization: Processes and Prospects[J]. Mater. Sci. and Eng., 1994, 1: 69–74

    Article  Google Scholar 

  14. O N Tretinnikov, K Kato, Y Ikada. In Vitro Hydroxyapatite Deposition onto a Film Surface-grafted with Organophosphate Polymer[J]. J. Biomed Mater. Res., 1994, 28: 1 365–1 373

    Article  CAS  Google Scholar 

  15. Y Abe, T Kokubo, T Yamamura. Apatite Coating on Ceramics, Metal and Polymers Utilizing a Biological Process[J]. J. Mater. Sci. Mater. Med., 1990, 1: 233–238

    Article  CAS  Google Scholar 

  16. E Dalas, J K Kallitis, P G Koutsoukos. Crystallization of Hydroxyapatite on Polymers[J]. Langmuir, 1991, 7: 1 822–1 826

    Article  CAS  Google Scholar 

  17. S Viala, M Freche, J L Lacout. Effect of Chitosan on Octacalcium Phosphate Crystal Growth[J]. Carbohydr. Polym., 1996, 29: 197–201

    Article  CAS  Google Scholar 

  18. T Akazawa, M Kobayashi, T Kanno. Characterization of Albumin- and Lysozyme-adsorption Evaluated on Two Differently Prepared Apatite[J]. J. Mater. Sci., 1998, 33: 1 927–1 931

    Article  CAS  Google Scholar 

  19. F Feng, Y Liu, B Y Zhao, et al. In Vitro Biomineralization of Glutaraldehyde Crosslinked Chitosan Films[J]. J. Wuhan Univ. of Technol., 2005, 20(2): 20–23

    Article  CAS  Google Scholar 

  20. C A W Andrew, E Khor, G W Hastings. The Influence of Anionic Chitin Derivatives on Calcium Phosphate Crystallization[J]. Biomaterials, 1998, 19: 1 309–1 316

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Metal Matrix Composites, Shanghai Jiaotong University, Shanghai, 200030, China

    Fang Feng  (冯芳), Yu Liu, Binyuan Zhao & Keao Hu

  2. Department of Chemistry and Chemical Engineering, Suzhou Science and Technology University, Suzhou, 215011, China

    Fang Feng  (冯芳)

Authors
  1. Fang Feng  (冯芳)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Binyuan Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Keao Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fang Feng  (冯芳).

Additional information

Funded by the Natural Science Foundation of Shanghai (No. 04ZR14087)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feng, F., Liu, Y., Zhao, B. et al. In vitro biomineralization of glutaraldehyde crosslinked chitosan/glutamic acid films. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 24, 9–14 (2009). https://doi.org/10.1007/s11595-009-1009-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11595-009-1009-8

Key words

Search

Navigation