Hyaluronic acid (HA) was chemically modified by polyethylene glycol. Meanwhile, the dynamic mechanics properties of HA derivative and its viscoelastic changes were measured on 3ARES3 Rheometer (Japan) at 25 °C. Dried cross-linked films of 10×10 mm2 were immersed in phosphate buffered saline(PBS: pH 7.4) at 37 °C with different time periods to measure its water content and in vitro degradation. Moreover, cell cultured solutions, which were in the different cultivation vesse with 1 mg/mL Solution of HA derivative as doing experimental sample for 2 d, 4 d and 7 d, were observed, respectively, by an inverted discrepancy microscope. The cell relative growth rate was analyzed with the SPSS10.0 mathematic statistic software. Based on the above experiments, structure-modified HA derivative can meet the requirements of biomaterials in view of rheological and degradation in vitro and cytotoxicity charactereistics from clinical medical aspect under this experiment conditions.
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Funded by the 863 Project (No. 2002AA205091)
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Chen, J., Chen, J. & Xu, Z. Rheological and biological characteristics of hyaluronic acid derivative modified by polyethylene glycol. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 23, 617–621 (2008). https://doi.org/10.1007/s11595-007-5617-x