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Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta in Brazil and the description of three new species

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Abstract

Manihot esculenta, commonly known as cassava, is an important staple food in developing countries in Africa, Asia and Latin America. Despite its relevance, few surveys of endophytic fungi have been carried out on this plant. Nigrospora spp. have a wide range of hosts and can be found as saprobes, endophytes, and pathogens. Nigrospora has already been isolated from cassava plants in previous studies. However, its identification was made only at the genus level. In this study, healthy cassava stems were collected in the northeast region of Brazil from where specimens of this genus were obtained. The isolates were identified based on morphological features and phylogenetic analyses of internal transcribed spacer (ITS), part of the translation elongation factor 1 alpha (TEF1- α), and β-tubulin fragment (TUB2) genic regions. As a result, we report two new geographic occurrences of N. pyriformis, and N. vesicularifera, four new records of N. hainanensis, N. lacticolonia, N. pyriformis, and N. vesicularifera associated with cassava, and the description of three new species named N. endophytica, N. manihoticola and N. pernambucoensis.

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Data availability

Sequence data are deposited in GenBank (https://www.ncbi.nlm.nih.gov/genbank/), concatenated alignment is deposited in TreeBASE (http://www.treebase.org/). The ex-types and holotypes have been deposited in public collections (Micoteca URM Profa. Maria Auxiliadora de Queiroz Cavalcanti, and Herbário URM Pe. Camille Torrend, respectively) located at the Universidade Federal de Pernambuco (Recife, Brazil). New taxonomic descriptions and nomenclatures are deposited in MycoBank (www.mycobank.org).

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Acknowledgements

Amanda C.Q. Brito thanks the Fundação de Amparo à Ciência e Tecnologia de estado de Pernambuco (FACEPE) for a doctoral fellowship (2019−2023); A.R. Machado and C.M. Souza-Motta also acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the research fellowships. We are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Pró-Reitoria de Pesquisa e Inovação of the Universidade Federal de Pernambuco (PROPESQI-UFPE) for financial support, and Micoteca URM Profa. Maria Auxiliadora de Queiroz Cavalcanti of the Universidade Federal de Pernambuco for the assistance in the execution of this study.

Funding

This work was financial supported by research fellowship from CNPq-407539/2018–2; doctoral fellowship from FACEPE IBPG-0099–5.01/19; and financial supported by CAPES and PROPESQI-UFPE.

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Authors and Affiliations

  1. Departamento de Micologia, Universidade Federal de Pernambuco, Recife, 50740-600, Brazil

    Amanda Cupertino de Queiroz Brito, Juliana Ferreira de Mello, Ana Elisa de Almeida Souza, Sandy dos Santos Nascimento, Cristina Maria de Souza-Motta & Alexandre Reis Machado

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Contributions

All authors contributed to the conception and design of the manuscript. ARM guided and supervised this research. CMSM revised the data. ACQB, JFM, AEAS, SSN, and ARM collected the samples used in this study. JFM did the molecular lab work. ACQB performed the morphological and phylogenetic analyses, done the illustrations, and wrote the first draft. All authors contributed to its development and completion. All authors read and approved the final manuscript.

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Correspondence to Amanda Cupertino de Queiroz Brito.

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de Queiroz Brito, A.C., de Mello, J.F., de Almeida Souza, A.E. et al. Richness of Nigrospora spp. (Apiosporaceae) in Manihot esculenta in Brazil and the description of three new species. Mycol Progress 22, 37 (2023). https://doi.org/10.1007/s11557-023-01887-4

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