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Three new species of Lobothallia (Megasporaceae, Pertusariales, Ascomycota) from Pakistan and a new combination in the genus

Mycological Progress volume 21, Article number: 80 (2022) Cite this article

Abstract

Three new species Lobothallia elobulata, L. iqbalii, and L. pakistanica from Pakistan are described as new to science and illustrated. Lobothallia elobulata is characterized by the absence of lobes, the presence of a distinct black hypothallus, and small, almost sphaeric ascospores 9–12 × 5–8 μm. Lobothallia iqbalii is characterized by its crustose–placodioid thallus with large appressed apothecia, single on areole, a high hymenium, 100–150 μm tall, and absence of prothallus and secondary metabolites. Lobothallia pakistanica is characterized by its thin, closely adnate, lobate thallus, lecanorine–aspiciloid apothecia, 2–3 on areole, and the absence of secondary metabolites. A comparative morpho-anatomical study and ITS-based molecular analysis confirmed the position of the species within the genus Lobothallia.

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Data availability

Samples analyzed during this study have been deposited in the LAH Herbarium, University of the Punjab, Lahore. (https://vymaps.com/PK/LAH–Herbarium–Department–of–Botany–University–of–the–Punjab–Pakistan–334248940395482/).

Sequences generated during this study have been deposited in NCBI, Genbank data repository (https://www.ncbi.nlm.nih.gov/genbank/).

New taxa has been registered in Mycobank (https://www.mycobank.org/).

• (For list of accession numbers and voucher information, please see Appendix Table 2).

References

  • Ahmad S (1965) A preliminary contribution to the lichen-flora of West Pakistan. Biologia 11:21–47

  • Amrani S, Seaward MRD, Sipman HJM, Feuerer T (2018) Lichenological exploration of Algeria II: checklist of lichenized, lichenicolous and allied fungi. Herzog 31(2):817–892. https://doi.org/10.13158/heia.31.2.2018.817

    Article  Google Scholar 

  • Aptroot A, Iqbal SH (2012) Annotated checklist of the lichens of Pakistan, with reports of new records. Herzog 25(2):211–229

    Article  Google Scholar 

  • Flagey C (1896) Catalogue des lichens de l’Algérie. In: Battandier, J.A.; Trabut, L. Flore de l’Algérie. 2. pp. 1–140

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

    Article  CAS  PubMed  Google Scholar 

  • Hafellner J (1991) Die Gattung Aspicilia, ihre Ableitungen nebst Bemerkungen über cryptolecanorine Ascocarporganisation bei anderen Genera der Lecanorales (Ascomycetes lichenisati). Act Bot Malac 16:133–140

    Google Scholar 

  • Hall TA (1999) BioEdit: a user–friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp 41:95–98

    CAS  Google Scholar 

  • Hue A (1910) Lichenes morphologice et anatomice disposuit (continuatio). Nouv Ann Mus Hist Nat 2:1–120

    Google Scholar 

  • John V, Seaward MRD, Sipman HJM, Zedda L (2004) Lichens and lichenicolous fungi of Syria, including a first checklist. Herzog 17:157–177

    Google Scholar 

  • Khan M, Khalid AN, Lumbsch HT (2018) A new species of Lecidea (Lecanorales, Ascomycota) from Pakistan. Myco 38:25–34. https://doi.org/10.3897/mycokeys.38.26960

  • Kondratyuk SY, Gromakova AB, Khodosovtsev AY, Kim JA, Kondratyuk AS (2015) Agrestia zerovii (Megasporaceae, Lichenforming ascomycetes), a new species from South Eastern Europe proved by alternative phylogenetic analysis. Stud Bot Hung 46(2):69–94. https://doi.org/10.17110/StudBot.2015.46.2.69

  • Kondratyuk SY, Upreti DK, Mishra GK, Nayaka S, Ingle KK, Orlov OO., ... & Hur JS (2020) New and noteworthy lichen–forming and lichenicolous fungi 10. Acta Bot Hungar 62(1–2): 69–108. https://doi.org/10.1556/034.62.2020.1-2.6

  • Kou XR, Li SX, Ren Q (2013) Three new species and one new record of Lobothallia from China. Mycotax 123:241–249

    Article  Google Scholar 

  • Kumar P, Skouloudis AN, Bell M, Viana M, Carotta MC, Biskos G, Morawska L (2016) Real–time sensors for indoor air monitoring and challenges ahead in deploying them to urban buildings. Sci Total Environ 560:150–159. https://doi.org/10.1016/j.scitotenv.2016.04.032

    Article  CAS  PubMed  Google Scholar 

  • Magnusson AH (1939) Studies in species of Lecanora, mainly the Aspicilia gibbosa group. Kungl. Svenska Vetenskaps–Akademiens Handlingar, ser. III, 17, .5, 1–182

  • Marthinsen G, Rui S, Timdal E (2019) OLICH: A reference library of DNA barcodes for Nordic lichens. Bio Dat Jour 7

  • Müller J (1880) Enumeration lichenum Aegyptiacum. Rev Mycol 2:73–83

    Google Scholar 

  • Nordin A, Björn OL, Tibell L (2011) Two new Aspicilia species from Fennoscandia and Russia. Lichenol 43(1):27–37

  • Nordin A, Savić S, Tibell L (2010) Phylogeny and taxonomy of Aspicilia and Megasporaceae. Mycol 102:1339–1349

    Article  Google Scholar 

  • Orange A, James P, White FJ (2001) Microchemical methods for the identification of lichens. First Edit. British Lichen Society, London, 101 pp

  • Owe-Larsson B, Nordin A, Tibell L, Sohrabi M (2011) Circinaria arida sp. nova and the ‘Aspicilia desertorum’complex. Biblioth Lichenol 106:235–246

  • Paukov AG, Davydov EA, Nordin A, Roux C, Şenkardeşler A, Sohrabi M, Vondrák J, Frolov IV, Teptina AY, Shiryaeva AS (2019) Three new species, new combinations and a key to known species of Lobothallia (Megasporaceae). Lichenol 51(4):301–322. https://doi.org/10.1017/S0024282919000264

    Article  Google Scholar 

  • Roux C, Nordin A, Tibell L, Sohrabi M (2011) Some poorly known or new Aspicilia species from Pyrenees-Orientales (France). Bull Soc Linn Provence 14:177–227

  • Sambrook J, Russell DW (2006) Purification of nucleic acids by extraction with phenol: chloroform. Cold Spring Harb Protoc 2006(1):pdb–prot4455. https://doi.org/10.1101/pdb.prot4455

    Article  Google Scholar 

  • Schmitt I, Yamamoto Y, Lumbsch HT (2006) Phylogeny of Pertusariales (Ascomycotina): resurrection of Ochrolechiaceae and new circumscription of Megasporaceae. J Hatt Bot Lab 100:753–764

  • Sipman HJ, Aptroot A (2020) Ikaeria serusiauxii, a new Caloplaca-like lichen from Macaronesia and mainland Portugal, with a lichen checklist for Porto Santo. Pla and Fung Syst 65(1):120–130

  • Steiner J (1895) Ein Beitrag zur Flechtenflora der Sahara. Sitzungsber. Kaiserl. Akad. Wiss. Wien, Math.–Naturw. Cl., Abt. 1, 104: 384–393

  • Steiner J (1902) Zweiter Beitrag zur Flechtenflora Algiers. Verh. K. K. Zool.–Bot. Ges. Wien 52, 469–487

  • Valadbeigi T, Nordin A, Tibell L (2011) Megaspora rimisorediata (Pertusariales, Megasporaceae), a new sorediate species from Iran and its affinities with Aspicilia sensu lato. Lichenol 43(4):285–291

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic Press, Inc., New York, pp 315–322

    Google Scholar 

  • Zhang YY, Wang XY, Li LJ, Printzen C, Timdal E, Niu DL et al (2020) Squamarina (lichenised fungi) species described from China belong to at least three unrelated genera. Myco 66:135

Download references

Acknowledgements

AP is grateful to the herbarium of Vienna Museum of Natural History (W) and personally Anton Igersheim, and Philippe Clerc (Conservatoire et Jardin botaniques de la Ville de Genève, G) for their hospitality and the possibility to study specimens. The work of AP is partly supported by the grant of RSCF (# 23-44-00070).

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Authors and Affiliations

  1. Institute of Botany, Fungal Biology and Systematics Lab, University of the Punjab, Quaid–e–Azam Campus, Lahore, 54590, Pakistan

    Rizwana Zulfiqar, Fatima Razzaq, Najam-ul-Sehar Afshan, Iram Fayyaz, Kamran Habib & Abdul Nasir Khalid

  2. Institute of Natural Sciences and Mathematics, Ural Federal University, Ekaterinburg, Russian Federation, 620000

    Alexander G. Paukov

Authors
  1. Rizwana Zulfiqar
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  2. Fatima Razzaq
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  3. Najam-ul-Sehar Afshan
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  4. Iram Fayyaz
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  5. Kamran Habib
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  6. Abdul Nasir Khalid
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  7. Alexander G. Paukov
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Contributions

Material preparation, data collection, and the first draft of the manuscript were done by Rizwana Zulfiqar. Study conception and design was written by Najam–ul–sehar Afshan. Phylogenetic analysis was performed by Iram Fayyaz and Fatima Razzaq. Samples collection, field study, and morphology were done by Kamran Habib. Supervision and final corrections in the manuscript were done by Dr. Abdul Nasir Khalid and Dr. Alexander Paukov. All authors read and approved the final manuscript.

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Correspondence to Rizwana Zulfiqar.

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Appendix

Appendix

Table 1 A brief comparison of Pakistani Lobothallia spp. with related taxa
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Table 2 Voucher specimens and NCBI GenBank accession numbers of the sequences used in the phylogenetic analysis
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Zulfiqar, R., Razzaq, F., Afshan, NuS. et al. Three new species of Lobothallia (Megasporaceae, Pertusariales, Ascomycota) from Pakistan and a new combination in the genus. Mycol Progress 21, 80 (2022). https://doi.org/10.1007/s11557-022-01830-z

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  • DOI: https://doi.org/10.1007/s11557-022-01830-z

Keywords

  • Gilgit–Baltistan
  • Azad Jammu and Kashmir
  • Kohistan
  • Lichenized fungi
  • Taxonomy
  • ITS
  • Phylogeny