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Three new species of Lobothallia (Megasporaceae, Pertusariales, Ascomycota) from Pakistan and a new combination in the genus


Three new species Lobothallia elobulata, L. iqbalii, and L. pakistanica from Pakistan are described as new to science and illustrated. Lobothallia elobulata is characterized by the absence of lobes, the presence of a distinct black hypothallus, and small, almost sphaeric ascospores 9–12 × 5–8 μm. Lobothallia iqbalii is characterized by its crustose–placodioid thallus with large appressed apothecia, single on areole, a high hymenium, 100–150 μm tall, and absence of prothallus and secondary metabolites. Lobothallia pakistanica is characterized by its thin, closely adnate, lobate thallus, lecanorine–aspiciloid apothecia, 2–3 on areole, and the absence of secondary metabolites. A comparative morpho-anatomical study and ITS-based molecular analysis confirmed the position of the species within the genus Lobothallia.

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Data availability

Samples analyzed during this study have been deposited in the LAH Herbarium, University of the Punjab, Lahore. (–Herbarium–Department–of–Botany–University–of–the–Punjab–Pakistan–334248940395482/).

Sequences generated during this study have been deposited in NCBI, Genbank data repository (

New taxa has been registered in Mycobank (

• (For list of accession numbers and voucher information, please see Appendix Table 2).


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AP is grateful to the herbarium of Vienna Museum of Natural History (W) and personally Anton Igersheim, and Philippe Clerc (Conservatoire et Jardin botaniques de la Ville de Genève, G) for their hospitality and the possibility to study specimens. The work of AP is partly supported by the grant of RSCF (# 23-44-00070).

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Material preparation, data collection, and the first draft of the manuscript were done by Rizwana Zulfiqar. Study conception and design was written by Najam–ul–sehar Afshan. Phylogenetic analysis was performed by Iram Fayyaz and Fatima Razzaq. Samples collection, field study, and morphology were done by Kamran Habib. Supervision and final corrections in the manuscript were done by Dr. Abdul Nasir Khalid and Dr. Alexander Paukov. All authors read and approved the final manuscript.

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Correspondence to Rizwana Zulfiqar.

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Table 1 A brief comparison of Pakistani Lobothallia spp. with related taxa
Table 2 Voucher specimens and NCBI GenBank accession numbers of the sequences used in the phylogenetic analysis

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Zulfiqar, R., Razzaq, F., Afshan, NuS. et al. Three new species of Lobothallia (Megasporaceae, Pertusariales, Ascomycota) from Pakistan and a new combination in the genus. Mycol Progress 21, 80 (2022).

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  • Gilgit–Baltistan
  • Azad Jammu and Kashmir
  • Kohistan
  • Lichenized fungi
  • Taxonomy
  • ITS
  • Phylogeny