New data on morphology, physiology, and geographical distribution of Lignomyces vetlinianus, its identity with Lentinus pilososquamulosus, and sufficient phylogenetic distance from Le. martianoffianus

Abstract

Several specimens of putative Lentinus pilososquamulosus (including the type specimen) and Le. martianoffianus from Siberia and the Russian Far East associated with different hosts and collected during the past two decades were studied. Morphological examination of the studied specimens showed a close similarity to specimens of Lignomyces vetlinianus, a species originally described from Central Europe, but later discovered in European Russia, the Caucasus (Abkhazia), and the Urals. Cultures of Li. vetlinianus were characterized by growth and morphology, and their adaptation to various temperatures was evaluated. Growth rate of the strains at 25 °C varied between 1.2 and 3.1 mm/day; the majority of them could survive freezing at −20 °C and grew at temperature ranging from 5 to 35 °C. Comparative culture characters, mating compatibility, and ITS sequencing revealed that the specimens earlier identified as Le. pilososquamulosus or Far East Russian Le. martianoffianus (misapplied name) were identical to Li. vetlinianus. It was shown that the distribution area of Li. vetlinianus extends from Central Europe to the South (Caucasus) and through Western Siberia to the Russian Far East. Since several attempts of Le. pilososquamulosus holotype sequencing were unsuccessful, an epitype of this taxon, represented by a successfully sequenced old topotype specimen, was proposed. Le. pilososquamulosus is considered as synonym of Li. vetlinianus, following the principle of priority. A molecular study of true Le. martianoffianus (type specimen) supported its conspecificity with Panus lecomtei.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Data availability

A data availability statement is present within the text. The datasets generated as part of this project are presented in the Supplement Information (SI) (Online Resource 1); generated sequences were deposited to the GenBank (NCBI). The results on evolutionary divergence between Lignomyces sequences are presented in the SI (Online Resource 2). Alignment and BA tree were deposited in TreeBASE: http://purl.org/phylo/treebase/phylows/study/TB2:S27931.

References

  1. Bulakh EM (2015) Fungi of the forests of Far East of Russia. Dalnauka, Vladivostok [In Russian]

    Google Scholar 

  2. Bulakh EM, Vassiljeva NV, Erofeeva EA (2010) The first data about fungi basidiomycetes of the “Bureinskiy” nature reserve. Mikologiya i fitopatologiya 44:89–98 [In Russian]

    Google Scholar 

  3. Domański S (1964) Pleurotus vetlinianus sp. nov. Acta Societatis Botanicorum Poloniae 33:243–246

  4. Gardes M, Bruns TD (1993) ITS primers with enhanced specification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

    CAS  Article  PubMed  Google Scholar 

  5. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    CAS  Article  Google Scholar 

  6. Kalyaanamoorthy S, Minh BQ, Thomas KF, Wong TKF, Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14:587–589. https://doi.org/10.1038/nmeth.4285

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20:1160–1166. https://doi.org/10.1093/bib/bbx108

    CAS  Article  PubMed  Google Scholar 

  8. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    CAS  Article  Google Scholar 

  9. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 35:1547–1549

    CAS  Article  Google Scholar 

  10. Malkovský KM (1932) Über die europäischen Arten der Gattung Panus. Annales Mycologici 30:10–80

    Google Scholar 

  11. Moser M (1979) Über einige neue oder seltene Agaricales-Arten aus dem Pieniny und aus Biesczciade, Polen. Sydowia Beiheft 8:268–275

    Google Scholar 

  12. Nguyen L-T, Schmidt HA, Arndt von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/molbev/msu300

    CAS  Article  Google Scholar 

  13. Pegler DN (1983) The genus Lentinus: a world monograph. Kew Bulletin additional series X. L.:1–281

  14. Petersen RH, Psurtseva NV, Zmitrovich IV, Chachuła P, Arslanov S, Hughes KW (2015) Lignomyces, a new genus of pleurotoid Agaricomycetes. Mycologia 107:1045–1054. https://doi.org/10.3852/14-355

    CAS  Article  PubMed  Google Scholar 

  15. Psurtseva NV (2010) Conservation of medicinal mushrooms in the LE (BIN) culture collection. Int J Med Mushrooms 12:193–199. https://doi.org/10.1615/IntJMedMushr.v12.i2.100

    Article  Google Scholar 

  16. Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology. syy032. https://doi.org/10.1093/sysbio/syy032

  17. Red book of Croatian fungi / Ed Tkalčec Z, Mešić A (2008) Tiskara Zelina, Zagreb

  18. Sazanova KV, Psurtseva NV, Shavarda AL (2018) Cultural and metabolomic studies of a new phthalides producer Lignomyces vetlinianus. Int J Med Mushrooms 20:1031–1045. https://doi.org/10.1615/IntJMedMushrooms.2018028687

    Article  PubMed  Google Scholar 

  19. Seelan JSS, Justo A, Nagy LG, Grand EA, Redhead SA, Hibbett DS (2015) Phylogenetic relationships and morphological evolution in Lentinus, Polyporellus and Neofavolus, emphasizing southeastern Asian taxa. Mycologia 107:460–474. https://doi.org/10.3852/14-084

    Article  PubMed  Google Scholar 

  20. Stavishenko IV, Zmitrovich IV (2017) The first find of Lignomyces vetlinianus (Tricholomataceae, Agaricomycetes) in the Urals. Mikologiya i fitopatologiya 51:60–63

    Google Scholar 

  21. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035

  22. Thorn RG, Barron GL (1986) Nematoctonus and the tribe Resupinateae in Ontario, Canada. Mycotaxon 25:321–454

    Google Scholar 

  23. Thümen F (1877) Beiträge zur Pilze-Flora Sibiriens. Byulleten Moskovskogo obshchestva ispytateley prirody 54:128–152

    Google Scholar 

  24. Trifinopoulos JL-T, Nguyen A, von Haeseler BQ, Minh (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res 44:W232–W235. https://doi.org/10.1093/nar/gkw256

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. Vassilieva LN (1973) Agarikowie shlyapochnye griby (poryadok Agaricales) Primorskogo kraya [Die Blätterpilze und Röhrlinge (Agaricales) von Primorye Region]. Nauka, Leningrad [In Russian]

    Google Scholar 

  26. Zmitrovich IV, Malysheva VF, Malysheva EF (2009) The hyphal types of polyporoid and pleurotoid fungi: a terminology revision. Ukrainian botanical journal 66:71–87 [in Russian]

    Google Scholar 

  27. Zmitrovich IV, Bondartseva MA, Perevedentseva LG, Myasnikov AG, Kovalenko AE (2018) The Meruliaceae of Russia. II. Panus. Turczaninowia 21:29–44

    Article  Google Scholar 

Download references

Acknowledgements

The study was performed within the framework of the institutional (BIN RAS) research project “Biodiversity, ecology, structural and functional features of fungi and fungus-like protists” АААА-А19-119020890079-6, using equipment at The Core Facility Centre at the Komarov Botanical Institute RAS. The authors thank Vera Malysheva and Ilya Prikhod’ko for helpful advices on using online service programs for phylogenetic analysis, and anonymous reviewers for useful comments and suggestions on the manuscript improvement.

Funding

This work was partially funded by the Russian Foundation for Basic Research (project no. 15-04-06211).

Author information

Affiliations

Authors

Contributions

All authors contributed to the study conception and design. Material preparation and data collection were performed by Nadezhda Psurtseva, Ivan Zmitrovich, Eugenia Bulakh, and Jaya Seelan; analysis and discussions were performed by Nadezhda Psurtseva, Ivan Zmitrovich, Roland Petersen, and Karen Hughes. The first draft of the manuscript was written by Nadezhda Psurtseva and Ivan Zmitrovich and revised by Ronald Petersen and Karen Hughes. All authors commented on the previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Nadezhda V. Psurtseva.

Ethics declarations

Consent for publication

Not applicable.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Section Editor: Zhu-Liang Yang

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Psurtseva, N.V., Zmitrovich, I.V., Seelan, J.S.S. et al. New data on morphology, physiology, and geographical distribution of Lignomyces vetlinianus, its identity with Lentinus pilososquamulosus, and sufficient phylogenetic distance from Le. martianoffianus. Mycol Progress 20, 809–821 (2021). https://doi.org/10.1007/s11557-021-01701-z

Download citation

Keywords

  • Morphology
  • Culture characters
  • Mating study
  • ITS sequencing
  • Phylogeny
  • Distribution