New data on morphology, physiology, and geographical distribution of Lignomyces vetlinianus, its identity with Lentinus pilososquamulosus, and sufficient phylogenetic distance from Le. martianoffianus


Several specimens of putative Lentinus pilososquamulosus (including the type specimen) and Le. martianoffianus from Siberia and the Russian Far East associated with different hosts and collected during the past two decades were studied. Morphological examination of the studied specimens showed a close similarity to specimens of Lignomyces vetlinianus, a species originally described from Central Europe, but later discovered in European Russia, the Caucasus (Abkhazia), and the Urals. Cultures of Li. vetlinianus were characterized by growth and morphology, and their adaptation to various temperatures was evaluated. Growth rate of the strains at 25 °C varied between 1.2 and 3.1 mm/day; the majority of them could survive freezing at −20 °C and grew at temperature ranging from 5 to 35 °C. Comparative culture characters, mating compatibility, and ITS sequencing revealed that the specimens earlier identified as Le. pilososquamulosus or Far East Russian Le. martianoffianus (misapplied name) were identical to Li. vetlinianus. It was shown that the distribution area of Li. vetlinianus extends from Central Europe to the South (Caucasus) and through Western Siberia to the Russian Far East. Since several attempts of Le. pilososquamulosus holotype sequencing were unsuccessful, an epitype of this taxon, represented by a successfully sequenced old topotype specimen, was proposed. Le. pilososquamulosus is considered as synonym of Li. vetlinianus, following the principle of priority. A molecular study of true Le. martianoffianus (type specimen) supported its conspecificity with Panus lecomtei.

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Data availability

A data availability statement is present within the text. The datasets generated as part of this project are presented in the Supplement Information (SI) (Online Resource 1); generated sequences were deposited to the GenBank (NCBI). The results on evolutionary divergence between Lignomyces sequences are presented in the SI (Online Resource 2). Alignment and BA tree were deposited in TreeBASE:


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The study was performed within the framework of the institutional (BIN RAS) research project “Biodiversity, ecology, structural and functional features of fungi and fungus-like protists” АААА-А19-119020890079-6, using equipment at The Core Facility Centre at the Komarov Botanical Institute RAS. The authors thank Vera Malysheva and Ilya Prikhod’ko for helpful advices on using online service programs for phylogenetic analysis, and anonymous reviewers for useful comments and suggestions on the manuscript improvement.


This work was partially funded by the Russian Foundation for Basic Research (project no. 15-04-06211).

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All authors contributed to the study conception and design. Material preparation and data collection were performed by Nadezhda Psurtseva, Ivan Zmitrovich, Eugenia Bulakh, and Jaya Seelan; analysis and discussions were performed by Nadezhda Psurtseva, Ivan Zmitrovich, Roland Petersen, and Karen Hughes. The first draft of the manuscript was written by Nadezhda Psurtseva and Ivan Zmitrovich and revised by Ronald Petersen and Karen Hughes. All authors commented on the previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Nadezhda V. Psurtseva.

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Psurtseva, N.V., Zmitrovich, I.V., Seelan, J.S.S. et al. New data on morphology, physiology, and geographical distribution of Lignomyces vetlinianus, its identity with Lentinus pilososquamulosus, and sufficient phylogenetic distance from Le. martianoffianus. Mycol Progress 20, 809–821 (2021).

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  • Morphology
  • Culture characters
  • Mating study
  • ITS sequencing
  • Phylogeny
  • Distribution