Several specimens of putative Lentinus pilososquamulosus (including the type specimen) and Le. martianoffianus from Siberia and the Russian Far East associated with different hosts and collected during the past two decades were studied. Morphological examination of the studied specimens showed a close similarity to specimens of Lignomyces vetlinianus, a species originally described from Central Europe, but later discovered in European Russia, the Caucasus (Abkhazia), and the Urals. Cultures of Li. vetlinianus were characterized by growth and morphology, and their adaptation to various temperatures was evaluated. Growth rate of the strains at 25 °C varied between 1.2 and 3.1 mm/day; the majority of them could survive freezing at −20 °C and grew at temperature ranging from 5 to 35 °C. Comparative culture characters, mating compatibility, and ITS sequencing revealed that the specimens earlier identified as Le. pilososquamulosus or Far East Russian Le. martianoffianus (misapplied name) were identical to Li. vetlinianus. It was shown that the distribution area of Li. vetlinianus extends from Central Europe to the South (Caucasus) and through Western Siberia to the Russian Far East. Since several attempts of Le. pilososquamulosus holotype sequencing were unsuccessful, an epitype of this taxon, represented by a successfully sequenced old topotype specimen, was proposed. Le. pilososquamulosus is considered as synonym of Li. vetlinianus, following the principle of priority. A molecular study of true Le. martianoffianus (type specimen) supported its conspecificity with Panus lecomtei.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
A data availability statement is present within the text. The datasets generated as part of this project are presented in the Supplement Information (SI) (Online Resource 1); generated sequences were deposited to the GenBank (NCBI). The results on evolutionary divergence between Lignomyces sequences are presented in the SI (Online Resource 2). Alignment and BA tree were deposited in TreeBASE: http://purl.org/phylo/treebase/phylows/study/TB2:S27931.
Bulakh EM (2015) Fungi of the forests of Far East of Russia. Dalnauka, Vladivostok [In Russian]
Bulakh EM, Vassiljeva NV, Erofeeva EA (2010) The first data about fungi basidiomycetes of the “Bureinskiy” nature reserve. Mikologiya i fitopatologiya 44:89–98 [In Russian]
Domański S (1964) Pleurotus vetlinianus sp. nov. Acta Societatis Botanicorum Poloniae 33:243–246
Gardes M, Bruns TD (1993) ITS primers with enhanced specification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755
Kalyaanamoorthy S, Minh BQ, Thomas KF, Wong TKF, Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14:587–589. https://doi.org/10.1038/nmeth.4285
Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20:1160–1166. https://doi.org/10.1093/bib/bbx108
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 35:1547–1549
Malkovský KM (1932) Über die europäischen Arten der Gattung Panus. Annales Mycologici 30:10–80
Moser M (1979) Über einige neue oder seltene Agaricales-Arten aus dem Pieniny und aus Biesczciade, Polen. Sydowia Beiheft 8:268–275
Nguyen L-T, Schmidt HA, Arndt von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol Biol Evol 32:268–274. https://doi.org/10.1093/molbev/msu300
Pegler DN (1983) The genus Lentinus: a world monograph. Kew Bulletin additional series X. L.:1–281
Petersen RH, Psurtseva NV, Zmitrovich IV, Chachuła P, Arslanov S, Hughes KW (2015) Lignomyces, a new genus of pleurotoid Agaricomycetes. Mycologia 107:1045–1054. https://doi.org/10.3852/14-355
Psurtseva NV (2010) Conservation of medicinal mushrooms in the LE (BIN) culture collection. Int J Med Mushrooms 12:193–199. https://doi.org/10.1615/IntJMedMushr.v12.i2.100
Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology. syy032. https://doi.org/10.1093/sysbio/syy032
Red book of Croatian fungi / Ed Tkalčec Z, Mešić A (2008) Tiskara Zelina, Zagreb
Sazanova KV, Psurtseva NV, Shavarda AL (2018) Cultural and metabolomic studies of a new phthalides producer Lignomyces vetlinianus. Int J Med Mushrooms 20:1031–1045. https://doi.org/10.1615/IntJMedMushrooms.2018028687
Seelan JSS, Justo A, Nagy LG, Grand EA, Redhead SA, Hibbett DS (2015) Phylogenetic relationships and morphological evolution in Lentinus, Polyporellus and Neofavolus, emphasizing southeastern Asian taxa. Mycologia 107:460–474. https://doi.org/10.3852/14-084
Stavishenko IV, Zmitrovich IV (2017) The first find of Lignomyces vetlinianus (Tricholomataceae, Agaricomycetes) in the Urals. Mikologiya i fitopatologiya 51:60–63
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030-11035
Thorn RG, Barron GL (1986) Nematoctonus and the tribe Resupinateae in Ontario, Canada. Mycotaxon 25:321–454
Thümen F (1877) Beiträge zur Pilze-Flora Sibiriens. Byulleten Moskovskogo obshchestva ispytateley prirody 54:128–152
Trifinopoulos JL-T, Nguyen A, von Haeseler BQ, Minh (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res 44:W232–W235. https://doi.org/10.1093/nar/gkw256
Vassilieva LN (1973) Agarikowie shlyapochnye griby (poryadok Agaricales) Primorskogo kraya [Die Blätterpilze und Röhrlinge (Agaricales) von Primorye Region]. Nauka, Leningrad [In Russian]
Zmitrovich IV, Malysheva VF, Malysheva EF (2009) The hyphal types of polyporoid and pleurotoid fungi: a terminology revision. Ukrainian botanical journal 66:71–87 [in Russian]
Zmitrovich IV, Bondartseva MA, Perevedentseva LG, Myasnikov AG, Kovalenko AE (2018) The Meruliaceae of Russia. II. Panus. Turczaninowia 21:29–44
The study was performed within the framework of the institutional (BIN RAS) research project “Biodiversity, ecology, structural and functional features of fungi and fungus-like protists” АААА-А19-119020890079-6, using equipment at The Core Facility Centre at the Komarov Botanical Institute RAS. The authors thank Vera Malysheva and Ilya Prikhod’ko for helpful advices on using online service programs for phylogenetic analysis, and anonymous reviewers for useful comments and suggestions on the manuscript improvement.
This work was partially funded by the Russian Foundation for Basic Research (project no. 15-04-06211).
Consent for publication
Conflict of interest
The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Section Editor: Zhu-Liang Yang
About this article
Cite this article
Psurtseva, N.V., Zmitrovich, I.V., Seelan, J.S.S. et al. New data on morphology, physiology, and geographical distribution of Lignomyces vetlinianus, its identity with Lentinus pilososquamulosus, and sufficient phylogenetic distance from Le. martianoffianus. Mycol Progress 20, 809–821 (2021). https://doi.org/10.1007/s11557-021-01701-z
- Culture characters
- Mating study
- ITS sequencing