Abstract
Laccaria fagacicola and L. pallidorosea, two new species collected from subtropical broad-leaved forests, were described in this study with both molecular and morphological evidence. Laccaria fagacicola is characterized by its brownish orange basidioma and globose to subglobose, echinulate basidiospores with spines 1–2 μm long and 1–1.5 μm wide at base. Laccaria pallidorosea has a small and pinkish basidioma, and globose to subglobose, echinate basidiospores with spines 1.5–2 μm long and 2 μm wide at base. Phylogenetic analyses based on internal transcribed spacer (ITS) region indicated that L. fagacicola is sister to L. rubroalba and L. aurantia, while L. pallidorosea is related to L. versiforma and L. salmonicolor. These two new species are described, illustrated, and compared with closely related and morphologically similar species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballero M, Contu M (1987) Tassonomia ed ecologia del genere Laccaria Berk. & Br. (Basidiomycetes, Agaricales, Tricholomataceae) in Sardegna. Candollea 42:601–611
Ballero M, Contu M (1989) Inquadramento tassonomico delle specie europee del genere Laccaria Berk. & Br. Candollea 44:119–127
Berkeley MJ, Broome CE (1883) Notices of British fungi (1989–2027). Ann Mag nat Hist 12:370–374
Besson M, Kühner R (1971) Ultrastructure de la paroi sporique des Laccaria Berk. et Br. (Agaricales). C R Hebd Seances Acad Sci 272:1078–1081
Bon M (1983) Tricholomataceae de France et d’Europe occidentale (6 ème partie: tribu Clitocybeae Fay.) Clé monographique. Doc Mycol 13:1–53
Cho HJ, Park MS, Lee H, Oh SY, Wilson AW, Mueller GM, Lim YW (2018) A systematic revision of the ectomycorrhizal genus Laccaria from Korea. Mycologia 110:948–961. https://doi.org/10.1080/00275514.2018.1507542
Cho HJ, Lee H, Park MS, Park KH, Park JH, Cho Y, Kim C, Lim YW (2020) Two new species of Laccaria (Agaricales, Basidiomycota) from Korea. Mycobiology 48:1–8. https://doi.org/10.1080/12298093.2020.1786961
Clémençon H (1984) Kompendium der Blatterpilze VI. Laccaria. Z Mykol 50:3–12
Corrales A, Wilson AW, Mueller GM, Ovrebo C (2020) Novel Laccaria species from Juglandaceae forest in Panama with notes on their ecology. Front Microbiol 11:1597. https://doi.org/10.3389/fmicb.2020.01597
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for Basidiomycetes: application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Heinemann P (1964) Champignons récoltés au Congo par Madame M. Goossens-Fontana: VI. Laccaria. Bulletin du Jardin botanique de l'État a Bruxelles 34:309–312
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. https://doi.org/10.1093/molbev/mst010
Kjøller R (2006) Disproportionate abundance between ectomycorrhizal root tips and their associated mycelia. FEMS Microbiol Ecol 58:214–224. https://doi.org/10.1111/j.1574-6941.2006.00166.x
Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben, 3rd edn. Muster-Schmidt Verlag, Zürich und Göttingen
Kropp BR, Mueller GM (1999) Laccaria. In: Cairney JWG, Chambers SM (eds) Ectomycorrhizal fungi: key genera in profile. Springer Press, Berlin, pp 65–88
Kühner R (1980) Les Hyménomycètes agaricoides. Bull Mens Soc Linn Lyon 49:1–1027
Lahaie DG (1981) The genus Laccaria in the boreal forest of eastern Canada. Dissertation, University of Toronto
Li F (2020) Two new species of Laccaria from South China, with a note on Hodophilus glaberipes. Mycol Prog 19:525–539. https://doi.org/10.1007/s11557-020-01573-9
Luo X, Ye L, Chen J, Karunarathna SC, Xu JC, Hyde KD, Mortimer PE (2016) Laccaria rubroalba sp. nov. (Hydnangiaceae, Agaricales) from Southwestern China. Phytotaxa 284:041–050. https://doi.org/10.11646/phytotaxa.284.1.4
Martin F, Selosse MA (2008) The Laccaria genome: a symbiont blueprint decoded. New Phytol 180:296–310. https://doi.org/10.1111/j.1469-8137.2008.02613.x
Martin F, Aerts A, Ahrén D et al (2008) The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis. Nature 452:88–92. https://doi.org/10.1038/nature06556
May TW (1991) A taxonomic study of the Australian species of Laccaria [doctoral dissertation]. Monash University
Mueller GM (1984) New North American species of Laccaria (Agaricales). Mycotaxon 20:101–116
Mueller GM (1991) Laccaria longipes, a new North America species of the Laccaria laccata complex. Mycotaxon 40:145–150
Mueller GM (1992) Systematics of Laccaria (Agaricales) in the continental United States and Canada, with discussions on extralimital taxa and descriptions of extant types. Fieldiana, Botany, New Series 30:1–158
Mueller GM, Sundberg WJ (1981) A floristic study of Lacearia (Agaricales) in southern Illinois. Nova Hedwigia 34:577–597
Nylander JAA (2004) MrModeltest v2. Program dstributed by the Author. Evolutionary Biology Centre, Uppsala University, Uppsala
Osmundson TW, Cripps CL, Mueller GM (2005) Morphological and molecular systematics of Rocky Mountain alpine Laccaria. Mycologia 97:949–972. https://doi.org/10.1080/15572536.2006.11832746
Pázmány D (1994) Zur Systematik der Gattung Laccaria Bk. et Br. Z Mykol 60:5–12
Popa F, Rexer KH, Donges K, Yang ZL, Kost G (2014) Three new Laccaria species from Southwest China (Yunnan). Mycol Prog 13:1105–1117. https://doi.org/10.1007/s11557-014-0998-7
Popa F, Jimenéz SYC, Weisenborn J, Donges K, Rexer KH, Piepenbring M (2016) A new Laccaria species from cloud forest of Fortuna, Panama. Mycol Prog 15:19. https://doi.org/10.1007/s11557-015-1139-7
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Singer R (1967) Notes sur le genre Laccaria. Bull Trimest Soc Mycol Fr 83:104–123
Singer R (1986) The Agaricales in modern taxonomy. Koeltz Scientific Books, Koenigstein
Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Vellinga EC (1986) Some species of Laccaria from India. Sydowia 39:224–229
Vincenot L, Popa F, Laso F, Donges K, Rexer KH, Kost G, Yang ZL, Nara K, Selosse MA (2017) Out of Asia: biogeography of fungal populations reveals Asian origin of diversification of the Laccaria amethystina complex, and two new species of violet Laccaria. Fungal Biol 121:939–955. https://doi.org/10.1016/j.funbio.2017.08.001
Wang L, Yang ZL, Liu JH (2004) Two new species of Laccaria (Basidiomycetes) from China. Nova Hedwigia 79(3–4):511–517. https://doi.org/10.1127/0029-5035/2004/0079-0511
White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Wilson AW, Hosaka K, Perry BA, Mueller GM (2013) Laccaria (Agaricomycetes, Basidiomycota) from Tibet (Xizang Autonomous Region, China). Mycoscience 54:406–419. https://doi.org/10.1016/j.myc.2013.01.006
Wilson AW, Wickett NJ, Grabowski P, Fant J, Borevitz J, Mueller GM (2015) Examining the efficacy of a genotyping-by-sequencing technique for population genetic analysis of the mushroom Laccaria bicolor and evaluating whether a reference genome is necessary to assess homology. Mycologia 107:217–226. https://doi.org/10.3852/13-278
Wilson AW, Hosaka K, Mueller G (2017) Evolution of ectomycorrhizas as a driver of diversification and biogeographic patterns in the model mycorrhizal mushroom genus Laccaria. New Phytol 213:1862–1873. https://doi.org/10.1111/nph.14270
Wu F, Zhou LW, Yang ZL, Bau T, Li TH, Dai YC (2019) Resource diversity of Chinese macrofungi: edible, medicinal and poisonous species. Fungal Divers 98:1–76. https://doi.org/10.1007/s13225-019-00432-7
Acknowledgements
We are grateful to Dr. Qi Zhao (Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China) and Dr. Li-Ping Tang (Kunming Medical University, Kunming, China) for their valuable specimens. The authors thank Zhi-Jia Gu for providing SEM images. Thanks are also due to the anonymous reviewers for their professional suggestions and comments. This work is supported by the biodiversity investigation, observation, and assessment program (2019–2023) of Ministry of Ecology and Environment of China, and postdoctoral directional training foundation of Yunnan province.
Availability of data and material
The sequences generated in this study are available in the NCBI GenBank under the accession numbers explained in the text. The specimens studied in this research were deposited in the Herbarium of Cryptogams, Kunming Institute of Botany, Chinese Academy of Sciences (HKAS).
Funding
This work is supported by the biodiversity investigation, observation, and assessment program (2019–2023) of Ministry of Ecology and Environment of China, and Postdoctoral Directional Training Foundation of Yunnan Province.
Author information
Authors and Affiliations
Contributions
All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Yang-Yang Cui, Qing Cai, Jing Li, and Zhu L. Yang. The first draft of the manuscript was written by Yang-Yang Cui and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics approval
Not applicable
Consent to participate
Not applicable
Consent for publication
Not applicable
Conflicts of interest
The authors declare no competing interests.
Additional information
Section Editor: Marc Stadler
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Cui, YY., Cai, Q., Li, J. et al. Two new Laccaria species from China based on molecular and morphological evidence. Mycol Progress 20, 567–576 (2021). https://doi.org/10.1007/s11557-021-01698-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11557-021-01698-5