Dominikia bonfanteae and Glomus atlanticum, two new species in the Glomeraceae (phylum Glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

Abstract

Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Part of this work was supported by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, as well as by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) that provided research grants to BT Goto and financial support in the project “Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota): taxonomy, systematic and evolution” (proc.408011-2016-5). We also thank Universidade Federal do Rio Grande do Norte for covering the costs of B.T. Goto’s stay as collaborative research in West Pomeranian University of Technology in Szczecin in the period from December 2019 to January 2020. The research was funded, in part, by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. The authors also thank two anonymous reviewers and the section editor, Dr. Marco Tines, for their constructive comments.

Funding

To Piotr Niezgoda by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, to Bruno Tomio Goto by Conselho Nacional de Desenvolvimento Científico e Tecnológico, proc. 408011-2016-5), to Szymon Zubek by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda and Szymon Zubek. The first draft of the manuscript was written by Janusz Błaszkowski and all authors commented on previous versions of the manuscript. Conceptualisation: Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno; methodology: Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Piotr Niezgoda; formal analysis and investigation: Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda, and Szymon Zubek; writing—original draft preparation: Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno; writing—review and editing: Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda, Szymon Zubek; funding acquisition: Bruno Tomio Goto, Piotr Niezgoda, Szymon Zubek; resources: Janusz Błaszkowski, Franco Magurno, Piotr Niezgoda; Supervision: Janusz Błaszkowski. All authors read and approved the final manuscript.

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The authors, Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda and Szymon Zubek, have no relevant financial or non-financial interests to disclose.

The authors, Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda and Szymon Zubek, have no conflicts of interest to declare that are relevant to the content of this article.

All authors, Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda and Szymon Zubek, certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript.

The authors, Janusz Błaszkowski, Bruno Tomio Goto, Franco Magurno, Monika Malicka, Edward Meller, Paweł Milczarski, Helena Moreira, Gabriela Woźniak, Piotr Niezgoda and Szymon Zubek, have no financial or proprietary interests in any material discussed in this article.

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Błaszkowski, J., Niezgoda, P., Zubek, S. et al. Dominikia bonfanteae and Glomus atlanticum, two new species in the Glomeraceae (phylum Glomeromycota) with molecular phylogenies reconstructed from two unlinked loci. Mycol Progress 20, 131–148 (2021). https://doi.org/10.1007/s11557-020-01659-4

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Keywords

  • Arbuscular mycorrhizal fungi
  • Morphology
  • Molecular phylogeny
  • RPB1
  • 18S-ITS-28S nrDNA
  • 18S-ITS-28S + RPB1