Many fungi have been identified as pathogens of marine algae. Among them, Chytridiomycota have been revealed as relatively highly abundant, but much of the diversity known within these groups is almost entirely based on environmental sequencing data. Here, we present a novel chytridiomycete genus and species, characterized by light microscopical observations, ultrastructure, and molecular phylogenetic analysis of the parasitic chytrid of brackish-water dinoflagellate Kryptoperidinium foliaceum from the Baltic Sea. Phylogenetic analysis of rDNA sequences and the ultrastructure of the strain reveals that it represents a new family in the order Rhizophydiales. Ericiomyces syringoforeus gen. et sp. nov. is a parasitoid with a life cycle composed by zoospores, which attach to the host, encyst, and produce a rhizoidal system (haustorium). Unlike typical Rhizophydiales chytrids, sporangium develops as a lateral outgrowth of the encysted zoospore. The ultrastructural study revealed at least two unique traits: the syringe-like organelle in the cyst, which supposed to paralyze the host, and funnel-shaped structure anchoring sporangium in the host wall. Sporangium matures and produces new zoospores within 3 days. Multiple infections are common and then the life cycle is 1–2 days shorter compared to the duration when a single infection occurred. Cross-infection experiments showed that E. syringoforeus could only infect dinoflagellates, being K. foliaceum highly susceptible to infection by the chytrid parasitoid. The effects of some fungal epidemics on populations of Kryptoperidinium are discussed.
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Light and electron microscopic studies and manuscript writing were supported by Russian Scientific Foundation grant 16-14-10302. SK and AV thank the Research Resource Centre for Molecular and Cell Technologies (RRC MCT) at St. Petersburg State University (SPbSU) for access to the EM facilities. EG, EA, and AR were supported by MINECO COPAS “Understanding top-down control in coastal bloom-forming protists” (CTM2017-86121-R). MK and KS were supported by JSPS KAKENHI grants 15KK0026 & 16H02943. AK and AP were supported by grant 251564 from Academy of Finland. The authors thank Dr. B.S.C. Leadbeater for English correction.
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Maximum likelihood phylogenetic tree of concatenated 18S + 5.8S + 28S rDNA sequences representing the diversity of Chytridiomycota. The sequence of Ericiomyces syringoforeus is in bold. Statistical support of the nodes is presented by the bootstrap value (%) and the Bayesian posterior probability. Only values >70% and > 0.95 respectively are shown. When only one of the values is below the threshold, it is indicated with a dashed line. (DOCX 26.0 kb)
Maximum likelihood phylogenetic tree of 28S rDNA sequences representing the diversity of the Rhizophydiales. The sequence of Ericiomyces syringoforeus is in bold. Statistical support of the nodes is presented by the bootstrap value (%) and the Bayesian posterior probability. Only values >70% and > 0.95 respectively are shown. When only one of the values is below the threshold, it is indicated with a dashed line. (DOCX 35.7 kb)
Maximum likelihood phylogenetic tree of 18S rDNA sequences representing the diversity of the Rhizophydiales. The sequence of Ericiomyces syringoforeus is in bold. Statistical support of the nodes is presented by the bootstrap value (%) and the Bayesian posterior probability. Only values >70% and > 0.95 respectively are shown. When only one of the values is below the threshold, it is indicated with a dashed line. (PDF 380 kb)
(DOCX 26 kb)
(DOCX 35.7 kb)
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Karpov, S.A., Reñé, A., Vishnyakov, A.E. et al. Parasitoid chytridiomycete Ericiomyces syringoforeus gen. et sp. nov. has unique cellular structures to infect the host. Mycol Progress 20, 95–109 (2021). https://doi.org/10.1007/s11557-020-01652-x
- Molecular phylogeny