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Are Trechisporales ectomycorrhizal or non-mycorrhizal root endophytes?

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Abstract

Trechispora (Hydnodontaceae) is considered as a soil-inhabiting fungus. However, some species in the genus are frequently forming basidiomes on soil, a typical feature of ectomycorrhizal fungi. Ectomycorrhizal basidiomes are found in neotropical and subtropical region, but taxonomical information and DNA sequences of root fungi and basidiomes from native Atlantic Rainforest are poorly reported. Basidiomes and soil samples including roots, humus layer, and mineral soil were collected in the Atlantic Rainforest, in Florianópolis (South of Brazil). Sequences of the ITS region were obtained from all sample types and subjected to phylogenetic reconstruction. Two sequences amplified from apparently ectomycorrhizal roots belonged to Trechispora and suggested a root-associated ecology, at least biotrophic and possibly ectomycorrhizal. The analysis of isotope abundance in the same Brazilian site and in French Guiana showed that Trechispora thelephora has high 15N abundance and is often intermediate between ectomycorrhizal and saprotrophic species in 13C abundance. This is congruent with a plant biotrophic ecology, perhaps ectomycorrhizal. Future investigations in subtropical regions are needed to determine whether such a mode of nutrition is widespread among Trechispora.

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Acknowledgments

The first author thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the master’s scholarship. We thank Lina Ribeiro Venturieri and Cony Decock for help on collecting field trips, Professor Mayara Caddah for help in Guapira opposita field identification, and one anonymous reviewer for insightful comments on an earlier version of this paper. Isotopic analyses were made at SSMIM (MNHN Paris) with technical assistance of Denis Fiorillo (UMR 7209 CNRS).

Funding

The sampling in French Guiana was funded by Nouragues field station projects and by Labex grants CEBA (ANR 10-LABX-0025) and TULIP (ANR 10-LABX-0041).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Biologia de Fungos, Algas e Plantas, Departamento de Botânica, Universidade Federal de Santa Catarina, Campus Universitário Trindade, Florianópolis, Santa Catarina, CEP: 88040-900, Brazil

    Mary Luz Vanegas-León & Maria Alice Neves

  2. Departamento de solos, Universidade Federal de Santa Maria, CCR Campus Universitário, Santa Maria, Rio Grande do Sul, 971050-900, Brazil

    Marcelo A. Sulzbacher

  3. Dipartimento di Scienze Biomediche, Università di Cagliari, Monserrato, Italy

    Andrea C. Rinaldi

  4. Laboratoire Evolution et Diversité Biologique, Paul Sabatier University - Toulouse III , UMR 5174 UPS-CNRS-ENFA, 118 route de Narbonne, 31062 Toulouse cedex 9, Toulouse, France

    Mélanie Roy

  5. Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum national d’Histoire naturelle, CNRS, Sorbonne Université, EPHE, CP 39, 57 rue Cuvier, 75005, Paris, France

    Marc-André Selosse

  6. Department of Plant Taxonomy and Nature Conservation, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland

    Marc-André Selosse

Authors
  1. Mary Luz Vanegas-León
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  2. Marcelo A. Sulzbacher
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  3. Andrea C. Rinaldi
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  4. Mélanie Roy
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  5. Marc-André Selosse
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  6. Maria Alice Neves
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Correspondence to Mary Luz Vanegas-León.

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Vanegas-León, M.L., Sulzbacher, M.A., Rinaldi, A.C. et al. Are Trechisporales ectomycorrhizal or non-mycorrhizal root endophytes?. Mycol Progress 18, 1231–1240 (2019). https://doi.org/10.1007/s11557-019-01519-w

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