Mycological Progress

, Volume 18, Issue 5, pp 641–652 | Cite as

Two new taxa of the Auriscalpium vulgare species complex with substrate preferences

  • Pan Meng Wang
  • Zhu L. YangEmail author
Original Article
Part of the following topical collections:
  1. Topical collection on Basidiomycote Mycology in honor of Franz Oberwinkler who passed away in March 2018


Basidiomes of the cone-inhabiting Auriscalpium species, usually regarded as A. vulgare Gray, are widely distributed across the Northern Hemisphere and easily recognized because of their unique macro-morphology. However, phylogenetic diversity patterns among different geographic populations and different substrates are completely unknown. In this study, samples of A. vulgare s.l. in different areas of China were studied phylogenetically, morphologically, and ecologically. For comparison, European collections of A. vulgare were included. Our phylogenetic analyses, inferred from partial nucleotide sequences including the internal transcribed spacers (ITS) 1 and 2 with the 5.8S nrDNA, the large subunit of nuclear ribosomal RNA (nrLSU), and the region between the conserved domain 6 and 7 of the gene for the second largest subunit of RNA polymerase II (RPB2), indicated that three phylogenetic species can be recognized, which correspond well with morphological and ecological evidence. Therefore, three species, having distinct substrate preferences, are documented here, namely the well-known A. vulgare, and the newly described species A. orientale and A. microsporum. Auriscalpium vulgare is widely distributed throughout the northern temperate Eurasia and North America, while the new species have been known mainly from subtropical East Asia and northeastern India to date.


Species diversity New species Substrate preferences 



We are grateful to Dr. Walter Till (Curator of University of Vienna Herbarium, WU) for sending us specimens of Auriscalpium vulgare on loan. We are very grateful to Drs. Hong Luo, Feng Bang, Gang Wu, Qing Cai, Jiao Qin, Qi Zhao, Yan Chun Li, Yan Jia Hao, Mr. Xiao Bin Liu, and Mr. Jian Wei Liu (Kunming Institute of Botany, KIB), Dr. Xue Tai Zhu (Northwest Normal University), and Dr. Li Ping Tang (Kunming Medical University) for providing us samples. We especially thank Drs. Gang Wu and Jiao Qin (KIB) for their constructive and illuminating comments, criticisms and suggestions.

Funding information

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31010000).

Supplementary material

11557_2019_1477_MOESM1_ESM.pdf (207 kb)
Fig. S1 Phylogenetic tree inferred from ML analysis based on the nrLSU alignment with G. strigosus (AF506449) and G. nigrescens (AF506450) as outgroups. Bootstrap values (BS > 75%), together with Bayesian Posterior Probabilities (PP > 0.95) are indicated above the branch (PDF 206 kb)
11557_2019_1477_MOESM2_ESM.pdf (170 kb)
Fig. S2 Phylogenetic tree inferred from ML analysis based on the RPB2 alignment with L. ursinus (KY495321) and L. cochleatus Karst. (KY495322) as outgroups. Bootstrap values (BS > 75%), together with Bayesian Posterior Probabilities (PP > 0.95) are indicated above the branch (PDF 169 kb)


  1. Berglund H, Hottola J, Siitonen J (2011) Linking substrate and habitat requirements of wood-inhabiting fungi to their regional extinction vulnerability. Ecography 34:864–875CrossRefGoogle Scholar
  2. Celio GJ, Padamsee M, Dentinger BT, Josephsen KA, Jenkinson TS, Mclaughlin EG, Mclaughlin DJ (2007) Septal pore apparatus and nuclear division of Auriscalpium vulgare. Mycologia 99:644–654CrossRefGoogle Scholar
  3. Cheng WC, Fu LK (1978) Flora of China. Vol. 7. Gymnospermae. Flora Reipublicae Popularis Sinicae Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita TomusGoogle Scholar
  4. Dai YC (2011) A revised checklist of corticioid and hydnoid fungi in China for 2010. Mycoscience 52:69–79CrossRefGoogle Scholar
  5. Das P, Chettri A, Kayang H (2009) Habitat preference of Auriscalpium vulgare Gray inhabiting slash and burn affected Khasi pine cones of India. Our Nat 7:32–38CrossRefGoogle Scholar
  6. Dettman JR, Jacobson DJ, Turner E, Pringle A, Taylor JW (2003) Reproductive isolation and phylogenetic divergence in Neurospora: comparing methods of species recognition in a model eukaryote. Evolution 57:2721–2741CrossRefGoogle Scholar
  7. Dettman JR, Jacobson DJ, Taylor JW (2006) Multilocus sequence data reveal extensive phylogenetic species diversity within the Neurospora discreta complex. Mycologia 98:436–446CrossRefGoogle Scholar
  8. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull 19:11–15Google Scholar
  9. Farjon A (2010) A handbook of the world’s conifers. Brill Academic Publishers, LeidenCrossRefGoogle Scholar
  10. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118CrossRefGoogle Scholar
  11. Gernandt DS, López GG, García SO, Liston A (2005) Phylogeny and classification of Pinus. Taxon 54:29–42CrossRefGoogle Scholar
  12. Gulis V (2001) Are there any substrate preferences in aquatic hyphomycetes? Mycol Res 105:1088–1093CrossRefGoogle Scholar
  13. Guo T, Wang HC, Xue WQ, Zhao J, Yang ZL (2016) Phylogenetic analyses of Armillaria reveal at least 15 phylogenetic lineages in China, seven of which are associated with cultivated Gastrodia elata. PLoS One 11:e0154794. CrossRefGoogle Scholar
  14. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  15. Hall D, Stuntz DE (1971) Pileate Hydnaceae of the Puget Sound Area. I. White-spored genera: Auriscalpium, Hericium, Dentinum and Phellodon. Mycologia 63:1099–1128CrossRefGoogle Scholar
  16. Kim CS, Jo JW, Kwag YN, Sung GH, Lee SG, Kim SY, Shin CH, Han SK (2015) Mushroom flora of Ulleung-gun and a newly recorded Bovista species in the Republic of Korea. Mycobiology 43:239–257CrossRefGoogle Scholar
  17. Kornerup A, Wanscher JH (1981) Taschenlexikon der farben 3. Aufl. Muster-Schmidt Verlag, Göttingen, pp 1–242Google Scholar
  18. Larsson E, Larsson KH (2003) Phylogenetic relationships of russuloid basidiomycetes with emphasis on aphyllophoralean taxa. Mycologia 95:1035–1065CrossRefGoogle Scholar
  19. Li N, Fu LK (1997) Notes on gymnosperms I. Taxonomic treatments of some Chinese conifers. Novon 7:261–264CrossRefGoogle Scholar
  20. Maas Geesteranus RA (1963) Hyphal structures in Hydnums II. Proc Kon Ned Akad Wetensch Series C 66:426–430Google Scholar
  21. Maas Geesteranus RA (1966) Notes on Hydnums IV. Proc Kon Ned Akad Wetensch Series C 69:329–333Google Scholar
  22. Martin L, Randlane T, Martin J (2011) Lichens and their substrate preferences on the pakri peninsula (Northwest Estonia). Folia Cryptog Estonica 48:45–58Google Scholar
  23. Matheny PB (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Mol Phylogenet Evol 35:1–20CrossRefGoogle Scholar
  24. Millanes AM, Truong C, Westberg M, Diederich P, Wedin M (2014) Host switching promotes diversity in host-specialized mycoparasitic fungi: uncoupled evolution in the Biatoropsis-Usnea system. Evolution 68:1576–1593CrossRefGoogle Scholar
  25. Miller SL, Larsson E, Larsson KH, Verbeken A, Nuytinck J (2006) Perspectives in the new Russulales. Mycologia 98:960–970CrossRefGoogle Scholar
  26. Nylander J (2004) MrModeltest 2.2. Comput Softw distributed by the Evol Biol Centre, Uppsala University, Uppsala, SwedenGoogle Scholar
  27. Pegler DN, Roberts PJ, Spooner BM (1997) British Chanterelles and tooth fungi. Europe Union by Continental Printing, BelgiumGoogle Scholar
  28. Petersen RH, Cifuentes J (1994) Notes on mating systems of Auriscalpium vulgare and A. villipes. Mycol Res 98:1427–1440CrossRefGoogle Scholar
  29. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefGoogle Scholar
  30. Roody WC (2003) Mushrooms of west Virginia and the central Appalachians. University Press of Kentucky, LexingtonGoogle Scholar
  31. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386Google Scholar
  32. Ryvarden L (2001) The genus Auriscalpium. Harv Pap Bot 6:193–198Google Scholar
  33. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690CrossRefGoogle Scholar
  34. Swofford DL (2002) PAUP *. Phylogenetic analysis using parsimony (*and other methods), 4.0b4a. Sinauer Associates, SunderlandGoogle Scholar
  35. Thomas K, Chilvers GA, Norris RH (1992) Aquatic hyphomycetes from different substrates: substrate preference and seasonal occurrence. Mar Freshw Res 43:491–509CrossRefGoogle Scholar
  36. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246CrossRefGoogle Scholar
  37. Vondrák J, Liška J (2010) Changes in distribution and substrate preferences of selected threatened lichens in the Czech Republic. Biologia 65:595–602CrossRefGoogle Scholar
  38. Wang PM, Liu XB, Dai YC, Horak E, Steffen K, Yang ZL (2018) Phylogeny and species delimitation of Flammulina: taxonomic status of winter mushroom in East Asia and a new European species identified using an integrated approach. Mycol Prog 7:1–18Google Scholar
  39. Werth S, Millanes AM, Wedin M, Scheidegger C (2013) Lichenicolous fungi show population subdivision by host species but do not share population history with their hosts. Fungal Biol 117:71–84CrossRefGoogle Scholar
  40. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: InnisMA GDH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  41. Wu ZY, Raven PH (1999) Flora of China, volume. Science Press, St. Louis, Missouri Botanical Garden, Beijing, p 4Google Scholar
  42. Zhang Y, Zhou DQ, Zhao Q, Zhou TX, Hyde KD (2010) Diversity and ecological distribution of macrofungi in the Laojun Mountain region, southwestern China. Biodivers Conserv 19:3545–3563CrossRefGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Chinese Academy of SciencesKunming Institute of BotanyKunmingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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