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Taxonomic and phylogenetic characterizations reveal two new species and two new records of Roussoella (Roussoellaceae, Pleosporales) from Yunnan, China

Abstract

Roussoella species mainly occur on monocotyledons, especially bamboo, and are characterized by raised, immersed ascostromata, cylindrical to clavate asci and ellipsoidal to fusiform, brown to dark brown, 1-septate, ornamented ascospores. They have cytospora-like asexual morphs with enteroblastic conidiogenous cells and hyaline to dark brown conidia, which are often minutely warty. In this article, two new species and two new records of Roussoella associated with dead bamboo, collected from Yunnan Province of China, are described and illustrated. These four taxa are similar in morphological characteristics, but can be distinguished by phylogenetic analyses of the concatenated ITS, LSU, TEF1-α, and RPB2 sequence data. Based on maximum likelihood and Bayesian inference analyses of the combined sequence dataset, we introduce Roussoella kunmingensis and R. yunnanensis spp. nov. Roussoella mukdahanensis is provided here as the first record on bamboo from China. The asexual morph of R. pseudohysterioides is illustrated and described on dead bamboo culms.

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Funding

We are grateful to The Mushroom Research Foundation, Chiang Mai, Thailand for supporting this research. We also acknowledge that Biology Experimental Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, who provide molecular laboratory facilities for us to extract DNA and amplify PCR fragments. Besides, we express thanks to the Center of Excellence in Fungal Research, Mae Fah Luang University and Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, for offering laboratory equipment to us in doing research on taxonomy. With the help of Prof. Shaun Pennycook, we successfully name the new species in genus Roussoella. Hongbo Jiang thank Mae Fah Luang University for offering The International Student Scholarship. Prof. Kevin D. Hyde thank the Foreign Experts Bureau of Yunnan Province, Foreign Talents Program (2018; grant no. YNZ2018002), Thailand Research grants entitled Biodiversity, phylogeny and role of fungal endophytes on above parts of Rhizophora apiculata and Nypa fruticans (grant no: RSA5980068), the future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants, Rhododendron species and Dracaena species (grant no: DBG6080013), Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion (grant no: RDG6130001) for supporting this study. Dr. Rungtiwa Phookamsak thank the Research Fund from China Postdoctoral Science Foundation (grant no. Y71B283261), the Yunnan Provincial Department of Human Resources and Social Security (grant no. Y836181261), and the Chiang Mai University and National Science Foundation of China (NSFC) project code 31850410489 for financial support.

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Jiang, HB., Hyde, K.D., Jayawardena, R.S. et al. Taxonomic and phylogenetic characterizations reveal two new species and two new records of Roussoella (Roussoellaceae, Pleosporales) from Yunnan, China. Mycol Progress 18, 577–591 (2019). https://doi.org/10.1007/s11557-019-01471-9

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Keywords

  • 2 new species
  • Bambusicolous fungi
  • Dothideomycetes
  • Morphology
  • Phylogeny