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Mycological Progress

, Volume 18, Issue 3, pp 359–368 | Cite as

Graphiola fimbriata: the first species of Graphiolaceae (Exobasidiales, Basidiomycota) described only based on its yeast stage

  • Shaghayegh NasrEmail author
  • Matthias Lutz
  • Mohammad Ali Amoozegar
  • Véronique Eparvier
  • Didier Stien
  • Seyed Abolhassan Shahzadeh Fazeli
  • Andrey Yurkov
Original Article
  • 172 Downloads

Abstract

The systematic position of three yeast strains isolated from a plant cell culture, a piece of termite nest, or as a foliar endophyte of Coffea arabica, respectively, is evaluated using morphological, physiological, and phylogenetical characteristics. In culture, all three isolates produced white, pale orange to pink colored colonies of cylindrical cells with monopolar budding and pseudohyphae. Standard phenotypic, biochemical, physiological characterization, and phylogenetic analyses of the combined 26S rRNA gene (D1/D2 domains) and ITS region sequences showed the conspecificity of these isolates and suggest their placement within the Exobasidiales (Ustilaginomycotina) as a sister lineage of the sampled and sequenced Graphiola species. Here, we describe this species as Graphiola fimbriata sp. nov. MycoBank MB 825077 (holotype: PC1T; ex-type cultures: IBRC-M 30158T = CBS 13945T = DSM 104832T). This is the first species described in the genus Graphiola for which only the asexual, saprobic developmental phase is known. The description of the genus Graphiola is therefore emended to allow species known only from a saprobic state.

Keywords

1 new taxon Graphiola fimbriata sp. nov. Asexual state Morphological characterization Phylogenetic analyses Yeast 

Notes

Acknowledgements

The authors are grateful to C. Nirma for isolating SNB-CN72 and to R. Constantino for identification of the host termite. Authors are grateful to Prof. M. Catherine Aime (Purdue University, USA) and Dr. Marizeth Groenewald (Westerdijk Fungal Biodiversity Institute, The Netherlands) for granting access to the strain IBL 03150.

Funding information

This work has benefited from an “Investissement d’Avenir” grant managed by the Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-0025).

The authors gratefully acknowledge financial support from the Iranian Biological Resource Centre (IBRC), ACECR.

Compliance with ethical standards

Conflicts of interest

The authors declare that there are no conflicts of interest.

Supplementary material

11557_2018_1450_MOESM1_ESM.docx (23 kb)
ESM 1 (DOCX 23 kb)

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Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Microorganisms Bank, Iranian Biological Resource Center (IBRC)ACECRTehranIran
  2. 2.Plant Evolutionary Ecology, Institute of Evolution and EcologyUniversity of TübingenTübingenGermany
  3. 3.Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of ScienceUniversity of TehranTehranIran
  4. 4.French National Centre for Scientific Research, Institut de Chimie des Substances Naturelles, UPR 2301Université Paris-SaclayGif-sur-YvetteFrance
  5. 5.CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, USR3579, Observatoire OcéanologiqueSorbonne UniversitéBanyuls-sur-MerFrance
  6. 6.Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in BiologyUniversity of Science and CultureTehranIran
  7. 7.Leibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesBrunswickGermany

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