Mycological Progress

, Volume 18, Issue 3, pp 321–334 | Cite as

Tricholomopsis badinensis sp. nov. and T. sulphureoides—two rare fungi of European old-growth forests

  • Jan HolecEmail author
  • Vladimír Kunca
  • Miroslav Kolařík
Original Article
Part of the following topical collections:
  1. Topical collection on Basidiomycote Mycology in honor of Franz Oberwinkler who passed away in March 2018


Interesting Tricholomopsis collections from old-growth forests in Slovakia and Poland were studied morphologically and molecularly using the ITS-LSU rDNA regions. Phylogenetic analysis of sequence data for all hitherto sequenced Tricholomopsis species revealed a geographic pattern. Species distributed primarily in the Holarctic are separated from those occurring in the Southern Hemisphere. Tricholomopsis decora is a species complex within which T. badinensis, a newly discovered European species, is described based on collections from fallen trunks of silver fir (Abies alba) in the Badínsky prales virgin forest in Slovakia. The species forms a well-supported clade in the phylogenetic tree. Morphologically, it is distinguished by medium-sized basidiomata, a brightly yellow to olive-yellow pileus with a tomentose, finely scaly to fibrillose covering which has an orange-yellow, yellow-brown to olive-brown color, scales adpressed for a long time and mostly ellipsoid basidiospores measuring 7–9(9.5) × (4)4.5–6 μm. Rich collections of T. sulphureoides from the Białowieża virgin forest in Poland are described, and the distribution and ecology of the species is summarized. The great biological value of the two aforementioned localities and the Dobročský prales virgin forest in Slovakia, a formerly published locality of T. sulphureoides (as T. osiliensis), is documented by a rich list of fungi considered old-growth forest indicators. A preliminary key for the identification of European Tricholomopsis species lacking red or violet coloration is proposed.


Basidiomycota Agaricales Phylogeny Taxonomy Ecology 



Trunk diameter at breast height (1.3 m)




Number of lamellae reaching the stipe


Number of lamellulae between each pair of lamellae


National Nature Reserve


Personal herbarium of V. Kunca


Quotient of length and width in any one basidiospore


Mean of basidiospore Q values of each individual collection measured



We thank D. Dvořák for providing data on his records and him and M. Kříž for providing photographs of T. sulphureoides. The work of J. Holec was financially supported by the Ministry of Culture of the Czech Republic (DKRVO 2018/08, National Museum, 00023272), and the research of V. Kunca was financially supported by the Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic (VEGA 1/0664/17 and 1/0104/19).


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Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jan Holec
    • 1
    Email author
  • Vladimír Kunca
    • 2
  • Miroslav Kolařík
    • 3
  1. 1.Mycological DepartmentNational MuseumPrague 9Czech Republic
  2. 2.Faculty of Ecology and Environmental Sciences, Department of Applied EcologyTechnical University in ZvolenZvolenSlovakia
  3. 3.Institute of MicrobiologyCzech Academy of SciencesPrague 4Czech Republic

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