Pseudopestalotiopsis dawaina sp. nov. and Ps. kawthaungina sp. nov.: two new species from Myanmar

  • Shunsuke Nozawa
  • Katsuhiko Ando
  • Nyunt Phay
  • Kyoko Watanabe
Original Article
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Abstract

Pestalotiopsis sensu lato comprises the genera Pestalotiopsis sensu stricto, Neopestalotiopsis, and Pseudopestalotiopsis, which form a taxonomic group originally called Pestalotiopsis and share a common conidial morph. However, the number of Pseudopestalotiopsis species is significantly lower than that of the other genera. In this study, we isolated two new species of this taxonomic group while performing a survey of Pestalotiopsis sensu lato in Myanmar, a tropical area. We performed molecular phylogenetic analyses of these new species by using a dataset that included sequences of the ribosomal internal transcribed spacer (ITS) region and the β-tubulin and translation elongation factor 1-alpha (tef1) genes and classified two isolates as members of Pseudopestalotiopsis. Phylogenetic trees indicated that each isolate was an independent species. Morphologically, the conidial traits of each of the two strains did not coincide with those of any previously reported species of the Pestalotiopsis sensu lato or Pestalotia genera. Descriptions and illustrations of the new species obtained in this study, Pseudopestalotiopsis dawaina and Ps. kawthaungina, are provided.

Keywords

ITS β-tubulin tef1 Phylogenetic analysis Morphological analysis Pestalotiopsis-like 

Notes

Compliance with ethical standards

All of the experiments performed in this study comply with the current laws of Myanmar and Japan.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11557_2018_1398_MOESM1_ESM.pptm (83 kb)
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11557_2018_1398_MOESM2_ESM.pptx (41 kb)
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References

  1. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefPubMedGoogle Scholar
  2. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  3. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120.  https://doi.org/10.1007/bf01731581 CrossRefPubMedGoogle Scholar
  4. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874.  https://doi.org/10.1093/molbev/msw054 CrossRefPubMedGoogle Scholar
  5. Lateef AA, Sepiah M, Bolhassan MH (2015) Description of Pseudopestalotiopsis kubahensis sp. nov., a new species of microfungi from Kubah National Park, Sarawak, Malaysia. Curr Res Environ Appl Mycol 5:376–381.  https://doi.org/10.5943/cream/5/4/8 CrossRefGoogle Scholar
  6. Liu E, Hou L, Raza M, Cai L (2017) Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China. Sci Rep 7:866.  https://doi.org/10.1038/s41598-017-00972-5 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Maharachchikumbura SSN, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EHC, Bahkali AH, Hyde KD (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Divers 56:95–129.  https://doi.org/10.1007/s13225-012-0198-1 CrossRefGoogle Scholar
  8. Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW (2014) Pestalotiopsis revisited. Stud Mycol 79:121–186.  https://doi.org/10.1016/j.simyco.2014.09.005 CrossRefPubMedPubMedCentralGoogle Scholar
  9. Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New YorkGoogle Scholar
  10. Nozawa S, Yamaguchi K, Yen LTH, Hop DV, Phay N, Ando K, Watanabe K (2017) Identification of two new species and a sexual morph from the genus Pseudopestalotiopsis. Mycoscience 58:328–337.  https://doi.org/10.1016/j.myc.2017.02.008 CrossRefGoogle Scholar
  11. Okada R, Kiyota E, Moriyama H, Fukuhara T, Natsuaki T (2014) A simple and rapid method to purify viral dsRNA from plant and fungal tissue. J Gen Plant Pathol 81:103–107.  https://doi.org/10.1007/s10327-014-0575-6 CrossRefGoogle Scholar
  12. Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences. Mycologia 97:84–98.  https://doi.org/10.1080/15572536.2006.11832842 PubMedGoogle Scholar
  13. Song Y, Tangthirasunun N, Maharachchikumbura SSN, Jiang Y, Xu J, Hyde KD, Wang Y (2014) Novel Pestalotiopsis species from Thailand point to the rich undiscovered diversity of this chemically creative genus. Cryptogam Mycol 35:139–149.  https://doi.org/10.7872/crym.v35.iss2.2014.139 CrossRefGoogle Scholar
  14. Steyaert RL (1949) Contributions etude monographique de Pestalotia de Not. al et Monochaetia Sacc. (Truncatella gen. nov. et Pestalotiopsis gen. nov.). Bull Jard Bot Brux 19:285–354CrossRefGoogle Scholar
  15. Watanabe K, Doi Y, Kobayashi T (1998) Conidiomatal development of Pestalotiopsis guepinii and P. neglecta on leaves of Gardenia jasminoides. Mycoscience 39:71–75.  https://doi.org/10.1007/s10267-011-0157-9 CrossRefGoogle Scholar
  16. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic press, San Diego, pp 315–322Google Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Shunsuke Nozawa
    • 1
  • Katsuhiko Ando
    • 2
  • Nyunt Phay
    • 3
  • Kyoko Watanabe
    • 1
  1. 1.Graduate School of AgricultureTamagawa UniversityMachidaJapan
  2. 2.National Institute of Technology and EvaluationKisarazuJapan
  3. 3.Pathein UniversityPatheinMyanmar

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