Abstract
Pestalotiopsis sensu lato comprises the genera Pestalotiopsis sensu stricto, Neopestalotiopsis, and Pseudopestalotiopsis, which form a taxonomic group originally called Pestalotiopsis and share a common conidial morph. However, the number of Pseudopestalotiopsis species is significantly lower than that of the other genera. In this study, we isolated two new species of this taxonomic group while performing a survey of Pestalotiopsis sensu lato in Myanmar, a tropical area. We performed molecular phylogenetic analyses of these new species by using a dataset that included sequences of the ribosomal internal transcribed spacer (ITS) region and the β-tubulin and translation elongation factor 1-alpha (tef1) genes and classified two isolates as members of Pseudopestalotiopsis. Phylogenetic trees indicated that each isolate was an independent species. Morphologically, the conidial traits of each of the two strains did not coincide with those of any previously reported species of the Pestalotiopsis sensu lato or Pestalotia genera. Descriptions and illustrations of the new species obtained in this study, Pseudopestalotiopsis dawaina and Ps. kawthaungina, are provided.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120. https://doi.org/10.1007/bf01731581
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Lateef AA, Sepiah M, Bolhassan MH (2015) Description of Pseudopestalotiopsis kubahensis sp. nov., a new species of microfungi from Kubah National Park, Sarawak, Malaysia. Curr Res Environ Appl Mycol 5:376–381. https://doi.org/10.5943/cream/5/4/8
Liu E, Hou L, Raza M, Cai L (2017) Pestalotiopsis and allied genera from Camellia, with description of 11 new species from China. Sci Rep 7:866. https://doi.org/10.1038/s41598-017-00972-5
Maharachchikumbura SSN, Guo LD, Cai L, Chukeatirote E, Wu WP, Sun X, Crous PW, Bhat DJ, McKenzie EHC, Bahkali AH, Hyde KD (2012) A multi-locus backbone tree for Pestalotiopsis, with a polyphasic characterization of 14 new species. Fungal Divers 56:95–129. https://doi.org/10.1007/s13225-012-0198-1
Maharachchikumbura SSN, Hyde KD, Groenewald JZ, Xu J, Crous PW (2014) Pestalotiopsis revisited. Stud Mycol 79:121–186. https://doi.org/10.1016/j.simyco.2014.09.005
Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York
Nozawa S, Yamaguchi K, Yen LTH, Hop DV, Phay N, Ando K, Watanabe K (2017) Identification of two new species and a sexual morph from the genus Pseudopestalotiopsis. Mycoscience 58:328–337. https://doi.org/10.1016/j.myc.2017.02.008
Okada R, Kiyota E, Moriyama H, Fukuhara T, Natsuaki T (2014) A simple and rapid method to purify viral dsRNA from plant and fungal tissue. J Gen Plant Pathol 81:103–107. https://doi.org/10.1007/s10327-014-0575-6
Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences. Mycologia 97:84–98. https://doi.org/10.1080/15572536.2006.11832842
Song Y, Tangthirasunun N, Maharachchikumbura SSN, Jiang Y, Xu J, Hyde KD, Wang Y (2014) Novel Pestalotiopsis species from Thailand point to the rich undiscovered diversity of this chemically creative genus. Cryptogam Mycol 35:139–149. https://doi.org/10.7872/crym.v35.iss2.2014.139
Steyaert RL (1949) Contributions etude monographique de Pestalotia de Not. al et Monochaetia Sacc. (Truncatella gen. nov. et Pestalotiopsis gen. nov.). Bull Jard Bot Brux 19:285–354
Watanabe K, Doi Y, Kobayashi T (1998) Conidiomatal development of Pestalotiopsis guepinii and P. neglecta on leaves of Gardenia jasminoides. Mycoscience 39:71–75. https://doi.org/10.1007/s10267-011-0157-9
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic press, San Diego, pp 315–322
Funding
This study was supported by JSPS KAKENHI, Grant Number 25440218 (Grant-in-Aid for Scientific Research C).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
All of the experiments performed in this study comply with the current laws of Myanmar and Japan.
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Section Editor: Marc Stadler
Rights and permissions
About this article
Cite this article
Nozawa, S., Ando, K., Phay, N. et al. Pseudopestalotiopsis dawaina sp. nov. and Ps. kawthaungina sp. nov.: two new species from Myanmar. Mycol Progress 17, 865–870 (2018). https://doi.org/10.1007/s11557-018-1398-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11557-018-1398-1