Mycological Progress

, Volume 17, Issue 7, pp 833–840 | Cite as

Host species effects on bacterial communities associated with the fruiting bodies of Tuber species from the Sichuan Province in Southwest China

  • Lei Ye
  • Qiang Li
  • Yu Fu
  • Pierre Sourzat
  • Hao Tan
  • Jie Zou
  • Bo Zhang
  • Xiaolin Li
Original Article


Tuber species produce highly sought-after truffles and host a wide diversity and high abundance of bacteria. It has been suggested that some of these bacteria contribute to the growth, maturity, and aromatic properties of truffles. Here, we characterized and compared the microbiomes of several species of truffles from the Sichuan Province in Southwest China using high-throughput sequencing of bacterial community 16S rRNA genes. Two T. pseudoexcavatum ascocarp samples had relatively similar bacterial communities, as indicated by PCoA analysis. In contrast, three T. indicum samples collected at different maturity stages did not contain similar communities, suggesting that the maturity stage of ascocarps affects community composition in addition to host phylogenetic background. Despite the variation seen among species and maturity stages, the Proteobacteria phylum dominated all communities, which is consistent with previous studies of Tuber-associated bacteria. Moreover, Bradyrhizobium was the dominant genus in most Tuber ascocarps, which is also consistent with previous studies, although the functional role of this genus within truffles is unclear. Notably, Serratia, which are essential producers of thiophene volatiles within T. borchii were dominant in all of our samples. This finding supports the hypothesis that the ability to produce thiophene volatiles is widespread among these bacteria.


Tuber species Bacteria High-throughput sequencing Bradyrhizobium Serratia 



This work was supported by the Applied Basic Research Program of Sichuan Province (2015JY0088), Science and Technology Support Project in Sichuan Province (2016NYZ0040), Science and Technology Projects for Richening People and Strengthening County in Sichuan Province (Huidong County special), as well as the Sichuan Mushroom Innovation Team. We would like to thank LetPub ( for providing linguistic assistance during the preparation of this manuscript.

Compliance with ethical standards

Conflict of interest statement

None declared.


  1. Antony-Babu S, Deveau A, Van Nostrand JD, Zhou J, Le Tacon F, Robin C, Frey-Klett P, Uroz S (2014) Black truffle-associated bacterial communities during the development and maturation of Tuber melanosporum ascocarps and putative functional roles. Environ Microbiol 16(9):2831–2847. CrossRefPubMedGoogle Scholar
  2. Barbieri E, Bertini L, Rossi I, Ceccaroli P, Saltarelli R, Guidi C, Zambonelli A, Stocchi V (2005) New evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus Tuber borchii Vittad. FEMS Microbiol Lett 247(1):23–35CrossRefPubMedGoogle Scholar
  3. Barbieri E, Ceccaroli P, Saltarelli R, Guidi C, Potenza L, Basaglia M, Fontana F, Baldan E, Casella S, Ryahi O (2010) New evidence for nitrogen fixation within the Italian white truffle Tuber magnatum. Fungal Biol 114(11–12):936–942CrossRefPubMedGoogle Scholar
  4. Barbieri E, Guidi C, Bertaux J, Frey-Klett P, Garbaye J, Ceccaroli P, Saltarelli R, Zambonelli A, Stocchi V (2007) Occurrence and diversity of bacterial communities in Tuber magnatum during truffle maturation. Environ Microbiol 9(9):2234–2246. CrossRefPubMedGoogle Scholar
  5. Benucci GMN, Bonito GM (2016) The truffle microbiome: species and geography effects on bacteria associated with fruiting bodies of hypogeous Pezizales. Microb Ecol 72:4–8. CrossRefPubMedGoogle Scholar
  6. Bonito GM, Gryganskyi AP, Trappe JM, Vilgalys R (2010) A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal. Mol Ecol 19(22):4994–5008CrossRefPubMedGoogle Scholar
  7. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M (2012) Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6(8):1621–1624CrossRefPubMedPubMedCentralGoogle Scholar
  8. Citterio B, Cardoni P, Potenza L, Amicucci A, Stocchi V, Gola G, Nuti M (1995) Isolation of bacteria from sporocarps of Tuber magnatum Pico, Tuber borchii Vittad. and Tuber maculatum Vitt.: identification and biochemical characterization. In: Stocchi V, Bonfante P, Nuti M (eds) Biotechnology of ectomycorrhizae: molecular approaches. Plenum, New York, pp 241–248CrossRefGoogle Scholar
  9. Duponnois R, Garbaye J (1990) Some mechanisms involved in growth stimulation of ectomycorrhizal fungi by bacteria. Botany-Botanique 68(10):2148–2152Google Scholar
  10. Fu Y, Li X, Li Q, Wu H, Xiong C, Geng Q, Sun H, Sun Q (2016) Soil microbial communities of three major Chinese truffles in Southwest China. Can J Microbiol 62(11):970–979CrossRefGoogle Scholar
  11. Gazzanelli G, Malatesta M, Pianetti A, Baffone W, Stocchi V, Citterio B (1999) Bacteria associated to fruit bodies of the ecto-mycorrhizal fungus Tuber borchii Vittad. Symbiosis 3:211–222Google Scholar
  12. Gryndler M, Soukupova L, Hrselova H, Gryndlerova H, Borovicka J, Streiblova E, Jansa J (2013) A quest for indigenous truffle helper prokaryotes. Environ Microbiol Rep 5(3):346–352. CrossRefPubMedGoogle Scholar
  13. Le Tacon F, Zeller B, Plain C, Hossann C, Brechet C, Robin C (2013) Carbon transfer from the host to Tuber melanosporum mycorrhizas and ascocarps followed using a 13C pulse-labeling technique. PLoS One 8(5):e64626CrossRefPubMedPubMedCentralGoogle Scholar
  14. Mello A, Ding GC, Piceno YM, Napoli C, Tom LM, DeSantis TZ, Andersen GL, Smalla K, Bonfante P (2013) Truffle brules have an impact on the diversity of soil bacterial communities. PLoS One 8(4):e61945. CrossRefPubMedPubMedCentralGoogle Scholar
  15. Mello A, Napoli C, Murat C, Morin E, Marceddu G, Bonfante P (2011) ITS-1 versus ITS-2 pyrosequencing: a comparison of fungal populations in truffle grounds. Mycologia 103(6):1184–1193. CrossRefPubMedGoogle Scholar
  16. Napoli C, Mello A, Borra A, Vizzini A, Sourzat P, Bonfante P (2010) Tuber melanosporum, when dominant, affects fungal dynamics in truffle grounds. New Phytol 185(1):237–247. CrossRefPubMedGoogle Scholar
  17. Sbrana C, Agnolucci M, Bedini S, Lepera A, Toffanin A, Giovannetti M, Nuti MP (2002) Diversity of culturable bacterial populations associated to Tuber borchii ectomycorrhizas and their activity on T. borchii mycelial growth. FEMS Microbiol Lett 211(2):195–201CrossRefPubMedGoogle Scholar
  18. Splivallo R, Deveau A, Valdez N, Kirchhoff N, Frey-Klett P, Karlovsky P (2015) Bacteria associated with truffle-fruiting bodies contribute to truffle aroma. Environ Microbiol 17(8):2647–2660. CrossRefPubMedGoogle Scholar
  19. Splivallo R, Ebeler SE (2015) Sulfur volatiles of microbial origin are key contributors to human-sensed truffle aroma. Appl Microbiol Biotechnol 99(6):2583–2592. CrossRefPubMedGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Soil and Fertilizer InstituteSichuan Academy of Agricultural SciencesChengduChina
  2. 2.College of Life ScienceSichuan UniversityChengduChina
  3. 3.Truffle Research Centre of Cahors-Le MontatLe MontatFrance

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