Mycological Progress

, Volume 17, Issue 7, pp 781–790 | Cite as

Three new species and one new combination of Gypsoplaca (lichenized Ascomycota) from the Hengduan Mountains in China

  • Haixia Shi
  • Xinyu Wang
  • Yuanfei Zhou
  • Dong Liu
  • Yanyun Zhang
  • Meixia Yang
  • Einar Timdal
  • Lisong Wang
Original Article


Three new species are described as follows: Gypsoplaca alpina H. X. Shi and Li S. Wang, G. bullata H. X. Shi and Li S. Wang, and G. rosulata H. X. Shi, Li S. Wang, and Timdal sp. nov. Gypsoplaca alpina is characterized by a small thallus, upper cortex with very thick gelatinized layer, ascocarp warty with a cerebriform ridging, and by growing on soil in alpine meadows. Gypsoplaca bullata characterized by having bullate ascocarp, uneven to bullate thallus. Gypsoplaca rosulata is characterized by rosulate thallus and 4–6-spored asci. The new combination Gypsoplaca blastidiata (Zhurb) H. X. Shi and Li S. Wang comb. & stat. nov. is proposed. A phylogenetic tree is established, based on combined sequences of ITS and nrLSU, and all species formed monophyletic clades. Two main chemical compounds, brialmontin and sesterterpenes, are reported for the first time from Gypsoplaca by analysis of NMR and MS data. Triterpenoids are absent in Chinese Gypsoplaca specimens.


Taxonomy Phylogeny Chemistry Gypsoplacaceae 



We thank Professor James Shevock from California Academy of Sciences and Professor Christoph Scheidegger from Swiss Federal Research Institute for providing numerous useful comments on the manuscript. We are grateful to Dr. Stefan Ekman and Dr. H. Thorsten Lumbsch at the Museum of Evolution, Uppsala (UPS), and the Field Museum of Natural History, Chicago (F), for the loan of specimens. We are also thankful to Dr. Jens Klackenberg at the Swedish Museum of Natural History (S), Stockholm, for providing the photos of the type specimens.

Funding information

This study was supported by a grant from the National Natural Science Foundation of China (No. 31670028, 31370069, 31400022, 31460005).

Supplementary material

11557_2018_1396_MOESM1_ESM.pdf (271 kb)
ESM 1 (PDF 270 kb)


  1. Abdulla A, Hurnisa X, Reyim M, Adilijiang A (2015) A new record of lichen family from Xinjiang. Arid Zone Res 32(3):509–511Google Scholar
  2. Arup U, Ekman S, Grube M, Mattsson J-E, Wedin M (2007) The sister group relation of Parmeliaceae (Lecanorales, Ascomycota). Mycologia 99:42–49CrossRefPubMedGoogle Scholar
  3. Crespo A, Lumbsch HT, Mattsson JE, Blanco O, Divakar PK, Articus K, Wiklund E, Bawingan PA, Wedin M (2007) Testing morphology-based hypotheses of phylogenetic relationships in Parmeliaceae (Ascomycota) using three ribosomal markers and the nuclear RPB1 gene. Mol Phylogenet Evol 44:812–824CrossRefPubMedGoogle Scholar
  4. Crespo A, Kauff F, Divakar PK et al (2010) Phylogenetic generic classification of parmelioid lichens (Parmeliaceae, Ascomycota) based on molecular, morphological and chemical evidence. Taxon 59:1735–1753Google Scholar
  5. Divakar PK, Crespo A, Wedin M et al (2015) Evolution of complex symbiotic relationships in a morphologically derived family of lichen-forming fungi. New Phytol 208:1217–1226CrossRefPubMedGoogle Scholar
  6. Divakar PK, Crespo A, Kraichak E et al (2017) Using a temporal phylogenetic method to harmonize family- and genus-level classification in the largest clade of lichen-forming fungi. Fungal Divers 84:101–117CrossRefGoogle Scholar
  7. Ekman S, Blaalid R (2011) The devil in the details: interactions between the branch-length prior and likelihood model affect node support and branch lengths in the phylogeny of the Psoraceae. Syst Biol 60(4):541–561CrossRefPubMedGoogle Scholar
  8. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application for the identification of mycorrhizae and rusts. Mol Ecol 2:113–118CrossRefPubMedGoogle Scholar
  9. Golubkova NS (1994) Gypsoplaca macrophylla (Gypsoplacaceae, Lichenes) in middle Asian and Mongolian floras. Z Naturforsch B 79(4):63–64Google Scholar
  10. Golubkova NS (2008) Gypsoplacaceae Timdal. In: Andreev et al. (eds) Handbook of the lichens of Russia. 10, Nauka, St. Petersburg, pp. 79–81Google Scholar
  11. Hafellner J, Türk R (2001) Die lichenisierten Pilze Österreichs - eine Checkliste der bisher nachgewiesenen Arten mit verbreitungsangaben. Stapfia 76:1–167Google Scholar
  12. Miadlikowska J, Kauff F, Hofstetter V et al (2006) New insights into classification and evolution of the Lecanoromycetes (Pezizomycotina,Ascomycota) from phylogenetic analyses of three ribosomal RNA- and two protein-coding genes. Mycologia 98:1088–1103CrossRefPubMedGoogle Scholar
  13. Miadlikowska J, Kauff F, Högnabba F et al (2014) Multigene phylogenetic synthesis for 1307 fungi representing 1139 infrageneric taxa, 312 genera and 66 families of the class Lecanoromycetes (Ascomycota). Mol Phylogenet Evol 79:132–168CrossRefPubMedGoogle Scholar
  14. Nylander JAA (2004) MrModeltest 2.3. Program distributed by the author. Evolutionary Biology Centre, Uppsala UniversityGoogle Scholar
  15. Poelt J, Gärtner G (1992) Gypsoplaca macrophylla, eine Flechte winterkalter Halbwüsten in den Alpen. Herz 9:229–237Google Scholar
  16. Rehner SA, Samuels GJ (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res 98(6):625–634CrossRefGoogle Scholar
  17. Resl P, Schneider K, Westberg M, Printzen C, Palice Z, Thor G, Fryday A, Mayrhofer H, Spribille T (2015) Diagnostics for a troubled backbone: testing topological hypotheses of trapelioid lichenized fungi in a large-scale phylogeny of Ostropomycetidae (Lecanoromycetes). Fungal Divers 73:239–258CrossRefPubMedPubMedCentralGoogle Scholar
  18. Schmull M, Miądlikowska J, Pelzer M, Stocker-Woergoetter E, Hofstetter V, Fraker E, Hodkinson BP, Reeb V, Kukwa M, Lumbsch HT, Kauff F, Lutzoni F (2011) Phylogenetic affiliations of members of the heterogeneous lichen-forming fungi of the genus Lecidea sensu Zahlbruckner (Lecanoromycetes, Ascomycota). Mycologia 103:983–1003CrossRefPubMedGoogle Scholar
  19. Seo C, Han Yim J, Kum Lee H et al (2011) PTP1B inhibitory secondary metabolites from the Antarctic lichen Lecidella carpathica. Mycology 2(1):18–23CrossRefGoogle Scholar
  20. Singh G, Divakar PK, Dal Grande F, Otte J, Parnmen S, Wedin M, Crespo A, Lumbsch HT, Schmitt I (2013) The sister-group relationships of the largest family of lichenized fungi, Parmeliaceae (Lecanorales, Ascomycota). Fungal Biol 117:715–721CrossRefPubMedGoogle Scholar
  21. Singh G, Dal Grande F, Divakar PK, Otte J, Leavitt SD, Szczepanska K, Crespo A, Rico VJ, Aptroot A, da Silva Cáceres ME, Lumbsch HT, Schmitt I (2015) Coalescent-based species delimitation approach uncovers high cryptic diversity in the cosmopolitan lichen-forming fungal genus Protoparmelia (Lecanorales, Ascomycota). PLoS One 10(5):e0124625CrossRefPubMedPubMedCentralGoogle Scholar
  22. Singh G, Dal Grande F, Divakar PK, Otte J, Crespo A, Schmitt I (2017) Fungal–algal association patterns in lichen symbiosis linked to macroclimate. New Phytol 214:317–329CrossRefPubMedGoogle Scholar
  23. Thell A, Miao V (1999) Phylogenetic analysis of ITS and intron sequences from European and American samples of cetrarioid lichens. Ann Bot Fenn 35:275–286Google Scholar
  24. Timdal E (1990) Gypsoplacaceae and Gypsoplaca, a new family and genus of squamiform lichens. Bibl Lichenologist 38:419–427Google Scholar
  25. Timdal E (1992, 1991) A monograph of the genus Toninia (Lecideaceae, Ascomycetes). Oper Bot 110: 1–137Google Scholar
  26. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172(8):4238–4246CrossRefPubMedPubMedCentralGoogle Scholar
  27. White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–321Google Scholar
  28. Zhurbenko M (2009) Lichenicolous fungi and some lichens from the Holarctic. Opusc Philolichenum 6:87–120Google Scholar
  29. Zhurbenko M, Daniëls FJA (2003) New or rarely reported lichenicolous fungi and lichens from the Canadian Arctic. Mycotaxon 88:97–106Google Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Haixia Shi
    • 1
  • Xinyu Wang
    • 1
  • Yuanfei Zhou
    • 2
  • Dong Liu
    • 1
  • Yanyun Zhang
    • 1
  • Meixia Yang
    • 1
  • Einar Timdal
    • 3
  • Lisong Wang
    • 1
  1. 1.Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  2. 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  3. 3.Natural History MuseumUniversity of OsloOsloNorway

Personalised recommendations