Comparative genome and transcriptome analysis reveal the medicinal basis and environmental adaptation of artificially cultivated Taiwanofungus camphoratus
- 141 Downloads
Taiwanofungus camphoratus is a widely used medicinal macrofungus unique to Taiwan, China, and it produces a diverse set of bioactive compounds. In this study, we resequenced the genome and transcriptome of artificially cultivated Taiwanofungus camphoratus and obtained its 29.7-Mb genome. Our aim was to elucidate the possible reasons for its medicinal value and its environmental adaptation from the genomic and evolutionary perspective. Compounds of triterpenoid family are highly abundant in Taiwanofungus camphoratus, and we identified 25 candidate genes that participated in the secondary metabolism leading to these valuable products. We observed fewer genes relating to the CAZymes family in Taiwanofungus camphoratus than in Ganoderma lucidum and Postia placenta, and these genes are considered beneficial for survival. Transcriptome sequencing revealed a large number of differentially expressed genes at various growth stages of Taiwanofungus camphoratus. Our data will be useful for studying the environmental adaptation of Taiwanofungus camphoratus and for developing a strategy to increase the production of its useful secondary metabolites.
KeywordsTaiwanofungus camphoratus Genome Transcriptome Triterpenoid production Environment adaptation
This study was supported by the National Science Foundation of China (grant No. 31572384) and the National High Technology Research and Development Program of China (grant No. 2011AA100901).
- Chen Y-T, Hong P-F, Wen L, Lin C-T (2014) Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata. Bot Stud 55(1). https://doi.org/10.1186/s40529-014-0077-z
- Fischer S et al (2011) Using OrthoMCL to assign proteins to OrthoMCL-DB groups or to cluster proteomes into new Ortholog groups. Curr Protoc Bioinformatics:6.12 11–16.12. 19. https://doi.org/10.1002/0471250953.bi0612s35
- Hattori M, Sheu C-C (2006) Compounds from Antrodia camphorata having anti-inflammatory and anti-tumor activity. US PatentsGoogle Scholar
- Tempel S (2012) Using and understanding RepeatMasker. Mobile Genetic Elements. 859:29–51. https://doi.org/10.1007/978-1-61779-603-6_2
- Wu S-H et al (2004) Taiwanofungus, a polypore new genus. Fungal Sci 19:109–116Google Scholar
- Yu Z-H, Wu S-H, Wang D-M, Chen C-T (2010) Phylogenetic relationships of Antrodia species and related taxa based on analyses of nuclear large subunit ribosomal DNA sequences. Bot Stud 51:1586–1591Google Scholar