Skip to main content

Morphology and multigene phylogeny reveal new genus and species of Torulaceae from freshwater habitats in northwestern Yunnan, China

Abstract

Current investigations on taxonomy of lignicolous freshwater fungi in northwestern Yunnan, China, have led to the discovery of new fungal species and records that belong to the family Torulaceae. In this paper, 21 fresh collections of Torulaceae from submerged wood in freshwater are characterized based on morphological characters and phylogenetic analyses of combined ITS, LSU, RPB2 and TEF1α sequence data. A new monotypic genus Rostriconidium represented by Rostriconidium aquaticum sp. nov., one new species of Torula (Torula aquatica), and a single new Dendryphion species (Dendryphion fluminicola) are introduced based on their distinct morphological features and evidence from molecular phylogeny. Multigene phylogeny also supports the family Torulaceae as a strongly supported monophyletic lineage with clear intergeneric boundaries basal to Roussoellaceae. Descriptions of Torula ficus and Torula masonii from the new collections are also provided, and they are first records for China.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  • Ahmed SA, Van De Sande WWJ, Stevens DA, Fahal A, van Diepeningen AD, Menken SBJ, de Hoog GS (2014) Revision of agents of blackgrain eumycetoma in the order Pleosporales. Persoonia 33:141–154

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Arambarri A, Cabello M, Mengascini A (1987) New hyphomycetes from Santiago River (Buenos Aires Province, Argentina). Mycotaxon 29:29–32

    Google Scholar 

  • Boehm EWA, Mugambi GK, Miller AN, Huhndorf SM, Marincowitz S, Spatafora JW, Schoch CL (2009) A molecular phylogenetic reappraisal of the Hysteriaceae, Mytilinidiaceae and Gloniaceae (Pleosporomycetidae, Dothideomycetes) with keys to world species. Stud Mycol 64:49–83

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Bucher VVC, Hyde KD, Pointing SB, Reddy CA (2004) Production of wood decay enzymes, mass loss and lignin solubilization in wood by diverse freshwater fungi. Microb Ecol 48:331–337

    CAS  Article  PubMed  Google Scholar 

  • Cai L, Zhang KQ, McKenzie EHC, Hyde KD (2002) Freshwater fungi from bamboo and wood submerged in the Liput River in the Philippines. Fungal Divers 13:1–12

    Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    CAS  Article  Google Scholar 

  • Chomnunti P, Hongsanan S, Aguirre-Hudson B, Tian Q, Peršoh D, Dhami MK, Alias AS, Xu JC, Liu XZ, Stadler M, Hyde KD (2014) The sooty moulds. Fungal Divers 66:1–36

    Article  Google Scholar 

  • Crane JL, Miller AN (2016) Studies in genera similar to Torula: Bahusaganda, Bahusandhika, Pseudotorula, and Simmonsiella gen. Nov. IMA Fungus 7:29–45

    Article  PubMed  PubMed Central  Google Scholar 

  • Crous PW, Shivas RG, Quaedvlieg W, van der Bank JT, Zhang Y, Summerell BA, Guarro J, Wingfield MJ, Wood AR, Alfenas AC, Braun U, Cano-Lira JF, Garcia D, Marin-Felix Y, Alvarado P, Andrade JP, Armengol J, Assefa A, den Breeyen A, Camele I, Cheewangkoon R, De Souza JT, Duong TA, Esteve-Raventos F, Fournier J, Frisullo S, Garcia-Jimenez J, Gardiennet A, Gene J, Hernandez-Restrepo M, Hirooka Y, Hospenthal DR, King A, Lechat C, Lombard L, Mang SM, Marbach PA, Marincowitz S, Marin-Felix Y, Montano-Mata NJ, Moreno G, Perez CA, Perez Sierra AM, Robertson JL, Roux J, Rubio E, Schumacher RK, Stchigel AM, Sutton DA, Tan YP, Thompson EH, van der Linde E, Walker AK, Walker DM, Wickes BL, Wong PT, Groenewald JZ (2014) Fungal planet description sheets: 214–280. Persoonia 32:184–306

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Crous PW, Carris LM, Giraldo A, Groenewald JZ, Hawksworth DL, Hernández-Restrepo M, Wood AR (2015) The genera of fungi—fixing the application of the type species of generic names—G2: Allantophomopsis, Latorua, Macrodiplodiopsis, Macrohilum, Milospium, Protostegia, Pyricularia, Robillarda, Rotula, Septoriella, Torula and Wojnowicia. IMA Fungus 6:163–198

    Article  PubMed  PubMed Central  Google Scholar 

  • Crous PW, Wingfield MJ, Burgess TI, Hardy GE, Crane C, Barrett S, Cano-Lira JF, Le Roux JJ, Thangavel R, Guarro J, Stchigel AM, Martín MP, Alfredo DS, Barber PA, Barreto RW, Baseia IG, Cano-Canals J, Cheewangkoon R, Ferreira RJ, Gené J, Lechat C, Moreno G, Roets F, Shivas RG, Sousa JO, Tan YP, Wiederhold NP, Abell SE, Accioly T, Albizu JL, Alves JL, Antoniolli ZI, Aplin N, Araújo J, Arzanlou M, Bezerra JD, Bouchara JP, Carlavilla JR, Castillo A, Castroagudín VL, Ceresini PC, Claridge GF, Coelho G, Coimbra VR, Costa LA, da Cunha KC, da Silva SS, Daniel R, de Beer ZW, Dueñas M, Edwards J, Enwistle P, Fiuza PO, Fournier J, García D, Gibertoni TB, Giraud S, Guevara-Suarez M, Gusmão LF, Haituk S, Heykoop M, Hirooka Y, Hofmann TA, Houbraken J, Hughes DP, Kautmanová I, Koppel O, Koukol O, Larsson E, Latha KP, Lee DH, Lisboa DO, Lisboa WS, López-Villalba Á, Maciel JL, Manimohan P, Manjón JL, Marincowitz S, Marney TS, Meijer M, Miller AN, Olariaga I, Paiva LM, Piepenbring M, Poveda-Molero JC, Raj KN, Raja HA, Rougeron A, Salcedo I, Samadi R, Santos TA, Scarlett K, Seifert KA, Shuttleworth LA, Silva GA, Silva M, Siqueira JP, Souza-Motta CM, Stephenson SL, Sutton DA, Tamakeaw N, Telleria MT, Valenzuela-Lopez N, Viljoen A, Visagie CM, Vizzini A, Wartchow F, Wingfield BD, Yurchenko E, Zamora JC, Groenewald JZ (2016) Fungal planet description sheets: 469–557. Persoonia 37:218–403

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Dai DQ, Phookamsak R, Wijayawardene NN, Li WJ, Bhat DJ, Xu JC, Taylor JE, Hyde KD, Chukeatirote E (2016) Bambusicolous fungi. Fungal Divers 82:1–105

    Article  Google Scholar 

  • Ellis MB (1971) Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew

    Google Scholar 

  • Glez-Peña D, Gómez-Blanco D, Reboiro-Jato M, Fdez-Riverola F, Posada D (2010) ALTER: program-oriented conversion of DNA and protein alignments. Nucleic Acids Res 38:14–18

    Article  Google Scholar 

  • Gnavi G, Garzoli L, Poli A, Prigione V, Burgaud G, Varese GC (2017) The culturable mycobiota of Flabellia petiolata: first survey of marine fungi associated to a Mediterranean green alga. PLoS One 12:e0175941

    Article  PubMed  PubMed Central  Google Scholar 

  • Goh TK, Hyde KD (1996) Biodiversity of freshwater fungi. J Ind Microbiol 17:328–345

    CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biologicalsequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hatakeyama S, Tanaka K, Harada Y (2008) Bambusicolous fungi in Japan (7): a new coelomycetous genus, Versicolorisporium. Mycoscience 49:211–214

    Article  Google Scholar 

  • Ho WH, Hyde KD, Hodgkiss IJ, Yanna (2001) Fungal communities on submerged wood from streams in Brunei, Hong Kong, and Malaysia. Mycol Res 105:1492–1501

    Article  Google Scholar 

  • Ho WH, Yanna, Hyde KD, Hodgkiss IJ (2002) Seasonality and sequential of fungi on wood submerged in Tai Po Kau Forest Stream, Hong Kong. Fungal Divers 10:21–43

    Google Scholar 

  • Hyde KD, Fryar S, Tian Q, Bahkali AH, Xu JC (2016a) Lignicolous freshwater fungi along a north-south latitudinal gradient in the Asian/Australian region; can we predict the affects of global warming on biodiversity and function? Fungal Ecol 19:190–200

    Article  Google Scholar 

  • Hyde KD, Hongsanan S, Jeewon R, Bhat DJ, McKenzie EHC, Jones EBG, Phookamsak R, Ariyawansa HA, Boonmee S, Zhao Q, Abdel-Aziz FA, Abdel-Wahab MA, Banmai S, Chomnunti P, Cui BK, Daranagama DA, Das K, Dayarathne MC, de Silva NI, Dissanayake AJ, Doilom M, Ekanayaka AH, Gibertoni TB, Go’es-Neto A, Huang SK, Jayasiri SC, Jayawardena RS, Konta S, Lee HB, Li WJ, Lin CG, Liu JK, Lu YZ, Luo ZL, Manawasinghe IS, Manimohan P, Mapook A, Niskanen T, Norphanphoun C, Papizadeh M, Perera RH, Phukhamsakda C, Richter C, de Santiago ALCMA, Drechsler-Santos ER, Senanayake IC, Tanaka K, TMDS T, Thambugala KM, Tian Q, Tibpromma S, Thongbai B, Vizzini A, Wanasinghe DN, Wijayawardene NN, Wu HX, Yang J, Zeng XY, Zhang H, Zhang JF, Bulgakov TS, Camporesi E, Bahkali AH, Amoozegar MA, Araujo-Neta LS, Ammirati JF, Baghela A, Bhatt RP, Bojantchev D, Buyck B, da Silva GA, de Lima CLF, de Oliveira RJV, de Souza CAF, Dai YC, Dima B, Duong TT, Ercole E, Mafalda-Freire F, Ghosh F, Hashimoto A, Kamolhan S, Kang JC, Karunarathna SC, Kirk PM, Kytövuori I, Lantieri A, Liimatainen K, Liu ZY, Liu XZ, Lucking R, Medardi G, Mortimer PE, Nguyen TTT, Promputtha I, Raj KNA, Reck MA, Lumyong S, Shahzadeh-Fazeli SA, Stadler M, Soudi MR, Su HY, Takahashi T, Tangthirasunun N, Uniyal P, Wang Y, Wen TC, Xu JC, Zhang ZK, Zhao YC, Zhou JL, Zhu L (2016b) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 80:1–270

    Article  Google Scholar 

  • Hyde KD, Norphanphoun C, Bazzicalupo A, Karunarathna A, Ekanayaka AH, Dissanayake AJ, Soares AM, Phukhamsakda C, Lin CG, Wanasinghe DN, Tennakoon DS, Abdel-Aziz FA, Lee H, Siedlecki I, Senanayake IC, Manawasinghe IS, Kušan I, Yang J, Li JF, Chethana KWT, Thambugala KM, He MQ, Mapook A, Clericuzio M, Dayarathne MC, Liu NG, Tian Q, Phookamsak R, Jayawardena RS, Tibpromma S, Huang SK, Hongsanan S, Konta S, Jayasiri SC, Abreu VP, Li V, Li WJ, Zeng XY, Xiao YP, Luo ZL, Daranagama DA, de Silva NI, Shang QJ, Perera RH, Vizzini A, Bahkali AH, Romero AI, Mešić A, Buyck B, Miller D, Camporesi E, Dovana F, Jr HLP, Promputtha I, Su HY, Bhat DJ, Jones EBG, McKenzie EHC, Kang JC, Liu JK, Xu JC, Pawłowski J, Ryvarden L, Jadan M, Wrzosek M, Berbee ML, Doilom M, Abdel-Wahab MA, Kim NK, Matočec N, Pereira OL, Chomnunti P, Zhao Q, Castañeda-Ruiz RF, Jeewon R, Zhao RL, Lumyong S, Maharachchikumbura SSN, Karunarathna SC, Boonmee S, Suetrong S, Gibertoni TB, Wen TC, Lim YW, Tkalčec Z, Liu ZY, Mortimer PE (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Divers 87:1–235

    Article  Google Scholar 

  • Index Fungorum 2018 – http://www.Indexfungorum.org/names/IndexFungorum Register.htm (February 2018)

  • Jaklitsch WM, Voglmayr H (2016) Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Stud Mycol 85:35–64

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Jayasiri SC, Hyde KD, Ariyawansa HA, Bhat DJ, Buyck B, Cai L, Dai YC, Abd-Elsalam KA, Ertz D, Hidayat I, Jeewon R, Jones EBG, Bahkali AH, Karunarathna SC, Liu JK, Luangsa-ard JJ, Lumbsch HT, Maharachchikumbura SSN, McKenzie EHC, Moncalvo JM, Ghobad-Nejhad M, Nilsson H, Pang KL, Pereira OL, Phillips AJL, Raspé O, Rollins AW, Romero AI, Etayo J, Selçuk F, Stephenson SL, Suetrong S, Taylor JE, Tsui CKM, Vizzini A, Abdel-Wahab MA, Wen TC, Boonmee S, Dai DQ, Daranagama DA, Dissanayake AJ, Ekanayaka AH, Fryar SC, Hongsanan S, Boonmee S, Jayawardena RS, Li WJ, Perera RH, Phookamsak R, Silva NI, Thambugala KM, Tian Q, Wijayawardene NN, Zhao RL, Zhao Q, Kang JC, Promputtha I (2015) The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Divers 74:3–18

    Article  Google Scholar 

  • Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa: recommendations to resolve taxonomic ambiguities. Mycosphere 7:1669–1677

    Article  Google Scholar 

  • Jeewon R, Liew ECY, Simpson JA, Hodgkiss IJ, Hyde KD (2003) Phylogenetic significance of morphological characters in the taxonomy of Pestalotiopsis species. Mol Phylogenet Evol 27:372–383

    CAS  Article  PubMed  Google Scholar 

  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Krauss GJ, Solé M, Krauss G, Schlosser D, Wesenberg D, Bärlocher F (2011) Fungi in freshwaters: ecology, physiology and biochemical potential. EMS. Microbiol Rev 35:620–651

    CAS  Google Scholar 

  • Li JF, Bhat DJ, Phookamsak R, Mapook A, Lumyong S, Hyde KD (2016) Sporidesmioides thailandica gen. et sp. nov. (Dothideomycetes) from northern Thailand. Mycol Prog 15:1169–1178

    Article  Google Scholar 

  • Li WL, Luo ZL, Liu JK, Bhat DJ, Bao DF, Su HY, Hyde KD (2017a) Lignicolous freshwater fungi from China I: Aquadictyospora lignicola gen. et sp. nov. and new record of Pseudodictyosporium wauense from northwestern Yunnan Province. Mycosphere 8:1587–1597

    Article  Google Scholar 

  • Li JF, Phookamsak R, Jeewon R, Bhat DJ, Mapook A, Camporesi E, Shang QJ, Chukeatirote E, Bahkali AH, Hyde KD (2017b) Molecular taxonomy and morphological characterization reveal new species and new host records of Torula species (Torulaceae, Pleosporales). Mycol Prog 16:447–461

    Article  Google Scholar 

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol Biol Evol 16:1799–1808

    CAS  Article  PubMed  Google Scholar 

  • Liu JK, Phookamsak R, Dai DQ, Tanaka K, Jones EBG, Xu JC, Chukeatirote E, Hyde KD (2014) Roussoellaceae, a new pleosporalean family to accommodate the genera Neoroussoella gen. nov., Roussoella and Roussoellopsis. Phytotaxa 181:1–33

    Article  Google Scholar 

  • Liu XY, Udayanga D, Luo ZL, Chen LJ, Zhou DQ, Su HY, Hyde KD (2015a) Backbone tree for Chaetothyriales with four new species of Minimelanolocus from aquatic habitats. Fungal Biol 119:1046–1062

    Article  PubMed  Google Scholar 

  • Liu JK, Hyde KD, Jones EBG, Ariyawansa HA, Bhat DJ, Boonmee S, Maharachchikumbura SSN, McKenzie EHC, Phookamsak R, Phukhamsakda C, Shenoy BD, Abdel-Wahab MA, Buyck B, Chen J, Chethana KWT, Singtripop C, Dai DQ, Dai YC, Daranagama DA, Dissanayake AJ, Doilom M, D’souza MJ, Fan XL, Goonasekara ID, Hirayama K, Hongsanan S, Jayasiri SC, Jayawardena RS, Karunarathna SC, Li WJ, Mapook A, Norphanphoun C, Pang KL, Perera RH, Peršoh D, Pinruan U, Senanayake IC, Somrithipol S, Suetrong S, Tanaka K, Thambugala KM, Tian Q, Tibpromma S, Udayanga D, Wijayawardene NN, Wanasinghe DN, Wisitrassameewong K, Zeng XY, Abdel-Aziz FA, Adamčík S, Bahkali AH, Boonyuen N, Bulgakov T, Callac P, Chomnunti P, Greiner K, Hashimoto A, Hofstetter V, Kang JC, Lewis D, Li XH, Liu XZ, Liu ZY, Matsumura M, Mortimer PE, Rambold G, Randrianjohany E, Sato G, Sri-Indrasutdhi V, Tian CM, Verbeken A, VonBrackel W, Wang Y, Wen TC, Xu JC, Yan JY, Zhao RL, Camporesi E (2015b) Fungal diversity notes 1–110: taxonomic and phylogenetic contributions to fungal species. Fungal Divers 72:1–197

    CAS  Article  Google Scholar 

  • Liu JK, Hyde KD, Jeewon R, Phillips AJL, Maharachchikumbura SSN, Ryberg M, Liu ZY, Zhao Q (2017) Ranking higher taxa using divergence times: a case study in Dothideomycetes. Fungal Divers 84:75–99

    Article  Google Scholar 

  • Luo J, Yin JF, Cai L, Zhang KQ, Hyde KD (2004) Freshwater fungi in Lake Dianchi, a heavily polluted lake in Yunnan, China. Fungal Divers 16:93–112

    Google Scholar 

  • Luo ZL, Bao DF, Bhat DJ, Yang J, Chai HM, Li SH, Bahkali AH, Su HY, Hyde KD (2016) Sporoschisma from submerged wood in Yunnan, China. Mycol Prog 15:1145–1155

    Article  Google Scholar 

  • Luo ZL, Bhat DJ, Jeewon R, Boonmee S, Bao DF, Zhao YC, Chai HM, Su HY, Su XJ, Hyde KD (2017) Molecular phylogeny and morphological characterization of asexual fungi (Tubeufiaceae) from freshwater habitats in Yunnan, China. Cryptogamie, Mycol 38:27–53

    Article  Google Scholar 

  • Luo ZL, Hyde KD, Bhat DJ, Jeewon R, Maharachchikumbura SSN, Bao DF, Li WL, Su XJ, Yang XY, Su HY (2018) Morphological and molecular taxonomy of novel species Pleurotheciaceae from freshwater habitats in Yunnan. China Mycol Prog. https://doi.org/10.1007/s11557-018-1377-6

  • Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In Gateway Computing Environments Workshop 2010 (GCE), New Orleans, Louisiana, November 2010, pp. 1–8

  • Mugambi GK, Huhndorf SM (2009) Molecular phylogenetics of Pleosporales: Melanommataceae and Lophiostomataceae recircumscribed (Pleosporomycetidae, Dothideomycetes, Ascomycota). Stud Mycol 64:103–121

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Nylander JAA (2004) MrModeltest v2 Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala

    Google Scholar 

  • Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Rannala B, Yang Z (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J Mol Evol 43:304–311

    CAS  Article  PubMed  Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic. Biol 61:539–542

    Google Scholar 

  • Schoch CL, Crous PW, Groenewald JZ, Boehm EWA, Burgess TI, de Gruyter J, de Hoog GS, Dixon LJ, Grube M, Gueidan C, Harada Y, Hatakeyama S, Hirayama K, Hosoya T, Huhndorf SM, Hyde KD, Jones EBG, Kohlmeyer J, Kruys A, Li YM, Lucking R, Lumbsch HT, Marvanová L, Mbatchou JS, McVay AH, Miller AN, Mugambi GK, Muggia L, Nelsen MP, Nelson P, Owensby CA, Phillips AJ, Phongpaichit S, Pointing SB, Pujade-Renaud V, Raja HA, Plata ER, Robbertse B, Ruibal C, Sakayaroj J, Sano T, Selbmann L, Shearer CA, Shirouzu T, Slippers B, Suetrong S, Tanaka K, Volkmann- Kohlmeyer B, Wingfield MJ, Wood AR, Woudenberg JH, Yonezawa H, Zhang Y, Spatafora JW (2009) A class-wide phylogenetic assessment of Dothideomycetes. Stud Mycol 64:1–15

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Seifert K, Morgan-Jones G, Gams W, Kendrick B (2011) The genera of hyphomycetes. CBS Biodiversity Series 9:1–997 CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands

  • Shenoy BD, Jeewon R, Wu WP, Bhat DJ, Hyde KD (2006) Ribosomal and RPB2 DNA sequence analyses suggest that Sporidesmium and morphologically similar genera are polyphyletic. Fungal Biol 110:916–928

    CAS  Google Scholar 

  • Shenoy BD, Jeewon R, Hyde KD (2007) Impact of DNA sequence data on the taxonomy of anamorphic fungi. Fungal Divers 26:1–54

    Google Scholar 

  • Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690

    CAS  Article  PubMed  Google Scholar 

  • Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic. Biol 75:758–771

    Google Scholar 

  • Sturm J (1829) Deutschlands Flora, Abt. III. Die Pilze Deutschlands 2:1–136

    Google Scholar 

  • Su HY, Udayanga D, Luo ZL, Manamgoda DS, Zhao YC, Yang J, Liu XY, McKenzie EHC, Hyde KD (2015) Hyphomycetes from aquatic habitats in Southern China: species of Curvularia (Pleosporaceae) and Phragmocephala (Melannomataceae). Phytotaxa 226:201–216

    Article  Google Scholar 

  • Su HY, Hyde KD, Maharachchikumbura SSN, Ariyawansa HA, Luo ZL, Promputtha I, Tian Q, Lin CG, Shang QJ, Zhao YC, Chai HM, Liu XY, Bahkali AH, Bhat DJ, McKenzie EHC, Zhou DQ (2016) The families Distoseptisporaceae fam.nov., Kirschsteiniotheliaceae, Sporidesmiaceae and Torulaceae, with new species from freshwater in Yunnan Province, China. Fungal Divers 80:375–409

    Article  Google Scholar 

  • Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, MA

  • Tanaka K, Mel’nik VA, Kamiyama M, Hirayama K, Shirouzu T (2010) Molecular phylogeny of two coelomycetous fungal genera with stellate conidia, Prosthemium and Asterosporium, on Fagales trees. Botany 88:1057–1071

    CAS  Article  Google Scholar 

  • Taylor JE, Hyde KD (2003) Microfungi on tropical and temperate palms. Fungal Divers Res. Ser 12:1–459

    Google Scholar 

  • Tibpromma S, Hyde KD, Jeewon R, Maharachchikumbura SSN, Liu JK, Bhat DJ, Jones EBG, McKenzie EHC, Camporesi E, Bulgakov TS, Doilom M, de Azevedo Santiago ALCM, Das K, Manimohan P, Gibertoni TB, Lim YW, Ekanayaka AH, Thongbai B, Lee HB, Yang JB, Kirk PM, Sysouphanthong P, Singh SK, Boonmee S, Dong W, Raj KNA, Latha KPD, Phookamsak R, Phukhamsakda C, Konta S, Jayasiri SC, Norphanphoun C, Tennakoon DS, Li JF, Dayarathne MC, Perera RH, Xiao Y, Wanasinghe DN, Senanayake IC, Goonasekara ID, de Silva NI, Mapook A, Jayawardena RS, Dissanayake AJ, Manawasinghe IS, Chethana KWT, Luo ZL, Hapuarachchi KK, Baghela A, Soares AM, Vizzini A, Meiras-Ottoni A, Mešić A, Dutta AK, de Souza CAF, Richter C, Lin CG, Chakrabarty D, Daranagama DA, Lima DX, Chakraborty D, Ercole E, Wu F, Simonini G, Vasquez G, da Silva GA, Plautz HL Jr, Ariyawansa HA, Lee H, Kušan I, Song J, Sun JZ, Karmakar J, Hu K, Semwal KC, Thambugala KM, Voigt K, Acharya K, Rajeshkumar KC, Ryvarden L, Jadan M, Hosen MI, Mikšík M, Samarakoon MC, Wijayawardene NN, Kim NK, Matočec N, Singh PN, Tian Q, Bhatt RP, de Oliveira RJV, Tulloss RE, Aamir S, Kaewchai S, Svetasheva STY, Nguyen TTT, Antonín V, Li WJ, Wang Y, Indoliya Y, Tkalčec Z, Elgorban AM, Bahkali AH, Tang AMC, Su HY, Zhang H, Promputtha I, Luangsa-ard J, Xu JC, Yan J, Chuan KJ, Stadler M, Mortimer PE, Chomnunti P, Zhao Q, Phillips AJL, Nontachaiyapoom S, Wen TC, Karunarathna SC (2017) Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 83:1–261

    Article  Google Scholar 

  • Tsui CKM, Hyde KD (2004) Biodiversity of fungi on submerged wood in a stream and its estuary in the Tai Ho Bay, Hong Kong. Fungal Divers 15:205–220

    Google Scholar 

  • Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis GM, Shinsky D, White T (eds) PCR protocols: a guide to methods and applications. Academic, New York, pp 315–322

    Google Scholar 

  • Wong KMK, Goh TK, Hodgkiss IJ, Hyde KD, Ranghoo VM, Tsui CKM, Ho WH, Wong SW, Yuen TK (1998) Role of fungi in freshwater ecosystems. Biodivers Conserv 7:1187–1206

    Article  Google Scholar 

  • Yuen TK, Hyde KD, Hodgkiss IJ (1998) Physiological growth parameters and enzyme production in tropical freshwater fungi. Mater Org 32:2–16

    Google Scholar 

  • Zhu D, Luo ZL, Bhat DJ, McKenzie EHC, Bahkali AH, Zhou DQ, Su HY, Hyde KD (2016) Helminthosporium velutinum and H. aquaticum sp. nov. from aquatic habitats in Yunnan Province, China. Phytotaxa 253:179–190

    Article  Google Scholar 

Download references

Acknowledgements

We would like to thank the National Natural Science Foundation of China (Project ID 31660008, 31460015, 31500014) and “Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China” for financial and laboratory support. Zong-Long Luo is grateful to Dr. Shaun Pennycook for advising on the taxon name and thank Ru-Xiao Wang, Qi-Shan Zhou, Zheng-Peng Li, and Hong-Wei Shen for their help on phylogeny and morphology work. Dr. R. Jeewon thanks Mae Fah Luang University for a visiting professorship and University of Mauritius for support.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hong-Yan Su.

Additional information

Section Editor: Kevin Hyde and Christiane Baschien

This article is part of the “Special Issue on Freshwater Ascomycota.”

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Su, XJ., Luo, ZL., Jeewon, R. et al. Morphology and multigene phylogeny reveal new genus and species of Torulaceae from freshwater habitats in northwestern Yunnan, China. Mycol Progress 17, 531–545 (2018). https://doi.org/10.1007/s11557-018-1388-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11557-018-1388-3

Keywords

  • Asexual fungi
  • One new genus
  • Phylogeny
  • Pleosporales
  • Three new species