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Comprehensive analysis of the volatilome of Scytinostroma portentosum

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Abstract

Fruiting bodies of the corticoid fungus Scytinostroma portentosum, known as mothball crust, have been sampled from a dead branch of sallow. Volatile organic compounds of the samples were extracted by means of liquid/liquid extraction and purified by solvent assisted flavour evaporation. In addition, solid-phase microextraction was applied on one fruiting body sample. The odour active compounds were identified by gas chromatography-mass spectrometry and olfactometry on two columns of different polarity and described by sensory detection. Furthermore, an aroma extract dilution analysis was performed to identify the main flavour compounds. The main odour compounds of S. portentosum with an FD factors ≥16 were 3-chloroindole responsible for the typical mothball odour, the mushroom odours oct-1-en-3-ol and oct-1-en-3-one, methyl p-anisate having an anise-like smell, the bloomy and sweet smelling terpenes linalool and nerolidol, as well as benzylacetone and methyl hexadecanoate. Herewith, a comprehensive aroma active volatilome of S. portentosum is presented.

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Acknowledgments

The study was financially supported by the excellence initiative of the Hessian Ministry of Science and Art, which encompasses a generous grant for the LOEWE focus “Integrative Fungal Research”. The authors also would like to thank the DFG and the State of Hesse for funding the GC-MS/MS-O system (INST 162/381-1 FUGG). The supportive work of Svenja Sommer, Frank Bernas and Stefan Riedel is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392, Giessen, Germany

    Vanessa Rühl, Anna Neuss, Holger Zorn & Martin Rühl

  2. Institute of Ecology, Evolution and Diversity, Cluster for Integrative Fungal Research (IPF), Goethe University Frankfurt, 60438, Frankfurt, Germany

    Hermine Lotz-Winter & Meike Piepenbring

Authors
  1. Vanessa Rühl
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  2. Hermine Lotz-Winter
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  3. Anna Neuss
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  4. Meike Piepenbring
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  5. Holger Zorn
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  6. Martin Rühl
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Correspondence to Martin Rühl.

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Section Editor: Marc Stadler

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Rühl, V., Lotz-Winter, H., Neuss, A. et al. Comprehensive analysis of the volatilome of Scytinostroma portentosum . Mycol Progress 17, 417–424 (2018). https://doi.org/10.1007/s11557-017-1367-0

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  • DOI: https://doi.org/10.1007/s11557-017-1367-0

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