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Multigene phylogeny reveals a new species and novel records and hosts in the genus Ramularia from Iran

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Abstract

Ramularia is a species-rich genus in the order Capnodiales (Dothideomycetes, Ascomycota) that includes numerous phytopathogenic taxa, several of which are economically important plant pathogens. In this study, six isolates of Ramularia were recovered from leaf spot symptoms on six herbaceous and woody plants from Guilan, East and West Azarbaijan provinces in the north and northwest of Iran. The isolates were studied by a polyphasic approach involving morphological and cultural data, and multi-gene phylogeny (ITS, TEF1-α, ACT, HIS, RPB2 and GAPDH). The isolates were grouped in three species clades of the R. eucalypti species complex. Of these, R. mali is recorded for the first time in Asia and R. glennii represents a new record for the mycobiota of Iran. Ramularia taleshina on Alnus subcordata is described as a new species. Ramularia taleshina is phylogenetically related to R. mali, but they can be differentiated by morphological and cultural characters as well as molecular data. Acalypha australis, Ficus carica and Platanus sp. are reported as new hosts of R. glennii, and Prunus cerasus and Vitis vinifera as new hosts of R. mali.

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Acknowledgements

We thank Prof. Pedro W. Crous, Dr. Johannes Z. Groenewald and Sandra I.R. Videira for their assistance. We further acknowledge the Research Deputy of the Iranian Research Institute of Plant Protection, the University of Tabriz, the Studienstiftung für mykologische Systematik und Ökologie, and the Westerdijk Fungal Biodiversity Institute for financial support.

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Authors and Affiliations

  1. Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 19395-1454, Tehran, Iran

    Mounes Bakhshi

  2. Plant Protection Department, Faculty of Agriculture, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran

    Mahdi Arzanlou

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  1. Mounes Bakhshi
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  2. Mahdi Arzanlou
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Correspondence to Mounes Bakhshi.

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Bakhshi, M., Arzanlou, M. Multigene phylogeny reveals a new species and novel records and hosts in the genus Ramularia from Iran. Mycol Progress 16, 703–712 (2017). https://doi.org/10.1007/s11557-017-1308-y

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  • DOI: https://doi.org/10.1007/s11557-017-1308-y

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